Cargando…
Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever
In September 2022, Panchkula Civil Hospital reported an outbreak of acute febrile illness (AFI) in Pinjore, located in the Himalayan foothills, Haryana, North India. There was an upsurge of fever cases. Blood samples were taken from suspected patients (n = 58) with AFI and subjected to serology of d...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637397/ https://www.ncbi.nlm.nih.gov/pubmed/37954851 http://dx.doi.org/10.3389/fphar.2023.1159377 |
_version_ | 1785133392531030016 |
---|---|
author | Satapathy, Prakasini Goel, Kapil Sharma, Vikrant Sarkar, Subhabrata Kang, Mannat Dhingra, Shefali Bora, Ishani Kaur, Kanwalpreet Arora, Neeraj Aggarwal, Arun Ratho, Radha Kanta |
author_facet | Satapathy, Prakasini Goel, Kapil Sharma, Vikrant Sarkar, Subhabrata Kang, Mannat Dhingra, Shefali Bora, Ishani Kaur, Kanwalpreet Arora, Neeraj Aggarwal, Arun Ratho, Radha Kanta |
author_sort | Satapathy, Prakasini |
collection | PubMed |
description | In September 2022, Panchkula Civil Hospital reported an outbreak of acute febrile illness (AFI) in Pinjore, located in the Himalayan foothills, Haryana, North India. There was an upsurge of fever cases. Blood samples were taken from suspected patients (n = 58) with AFI and subjected to serology of dengue, chikungunya, Japanese encephalitis, leptospira and scrub typhus. The samples were also screened for West Nile & Zika virus RNA using real-time PCR. Viral strains were characterized by sequencing. Of the 58 cases of AFI, Dengue could be identified in 45 (77.58%) followed by JE and Chikungunya in 2 cases each (3.44%), respectively. Among Dengue positive cases, 44 had monoinfection (97.77%) and 1 patient had dengue and JE. None were positive for Zika, West Nile, Scrub typhus, and Leptospira with the testing protocol. Four patients developed dengue with warning signs, such as abdominal pain in one patient and recurrent vomiting in the remaining three. The dengue serotype could be determined in 17 samples and revealed serotype 2. Molecular evolution analysis based on the complete envelope gene revealed that all DENV-2 strains (n = 13) circulated in the outbreak area belonged to the DENV-2 cosmopoliton genotype. In the early stages of infection, relying only on clinical manifestations is ineffective, so both molecular and serological assays along with clinical diagnosis are noteworthy for determining the aetiology of AFI. |
format | Online Article Text |
id | pubmed-10637397 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106373972023-11-11 Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever Satapathy, Prakasini Goel, Kapil Sharma, Vikrant Sarkar, Subhabrata Kang, Mannat Dhingra, Shefali Bora, Ishani Kaur, Kanwalpreet Arora, Neeraj Aggarwal, Arun Ratho, Radha Kanta Front Pharmacol Pharmacology In September 2022, Panchkula Civil Hospital reported an outbreak of acute febrile illness (AFI) in Pinjore, located in the Himalayan foothills, Haryana, North India. There was an upsurge of fever cases. Blood samples were taken from suspected patients (n = 58) with AFI and subjected to serology of dengue, chikungunya, Japanese encephalitis, leptospira and scrub typhus. The samples were also screened for West Nile & Zika virus RNA using real-time PCR. Viral strains were characterized by sequencing. Of the 58 cases of AFI, Dengue could be identified in 45 (77.58%) followed by JE and Chikungunya in 2 cases each (3.44%), respectively. Among Dengue positive cases, 44 had monoinfection (97.77%) and 1 patient had dengue and JE. None were positive for Zika, West Nile, Scrub typhus, and Leptospira with the testing protocol. Four patients developed dengue with warning signs, such as abdominal pain in one patient and recurrent vomiting in the remaining three. The dengue serotype could be determined in 17 samples and revealed serotype 2. Molecular evolution analysis based on the complete envelope gene revealed that all DENV-2 strains (n = 13) circulated in the outbreak area belonged to the DENV-2 cosmopoliton genotype. In the early stages of infection, relying only on clinical manifestations is ineffective, so both molecular and serological assays along with clinical diagnosis are noteworthy for determining the aetiology of AFI. Frontiers Media S.A. 2023-10-26 /pmc/articles/PMC10637397/ /pubmed/37954851 http://dx.doi.org/10.3389/fphar.2023.1159377 Text en Copyright © 2023 Satapathy, Goel, Sharma, Sarkar, Kang, Dhingra, Bora, Kaur, Arora, Aggarwal and Ratho. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Satapathy, Prakasini Goel, Kapil Sharma, Vikrant Sarkar, Subhabrata Kang, Mannat Dhingra, Shefali Bora, Ishani Kaur, Kanwalpreet Arora, Neeraj Aggarwal, Arun Ratho, Radha Kanta Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever |
title | Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever |
title_full | Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever |
title_fullStr | Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever |
title_full_unstemmed | Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever |
title_short | Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever |
title_sort | outbreak investigation of acute febrile illness from the himalayan foothills: solving the puzzle of fever |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637397/ https://www.ncbi.nlm.nih.gov/pubmed/37954851 http://dx.doi.org/10.3389/fphar.2023.1159377 |
work_keys_str_mv | AT satapathyprakasini outbreakinvestigationofacutefebrileillnessfromthehimalayanfoothillssolvingthepuzzleoffever AT goelkapil outbreakinvestigationofacutefebrileillnessfromthehimalayanfoothillssolvingthepuzzleoffever AT sharmavikrant outbreakinvestigationofacutefebrileillnessfromthehimalayanfoothillssolvingthepuzzleoffever AT sarkarsubhabrata outbreakinvestigationofacutefebrileillnessfromthehimalayanfoothillssolvingthepuzzleoffever AT kangmannat outbreakinvestigationofacutefebrileillnessfromthehimalayanfoothillssolvingthepuzzleoffever AT dhingrashefali outbreakinvestigationofacutefebrileillnessfromthehimalayanfoothillssolvingthepuzzleoffever AT boraishani outbreakinvestigationofacutefebrileillnessfromthehimalayanfoothillssolvingthepuzzleoffever AT kaurkanwalpreet outbreakinvestigationofacutefebrileillnessfromthehimalayanfoothillssolvingthepuzzleoffever AT aroraneeraj outbreakinvestigationofacutefebrileillnessfromthehimalayanfoothillssolvingthepuzzleoffever AT aggarwalarun outbreakinvestigationofacutefebrileillnessfromthehimalayanfoothillssolvingthepuzzleoffever AT rathoradhakanta outbreakinvestigationofacutefebrileillnessfromthehimalayanfoothillssolvingthepuzzleoffever |