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CIZ1 aggravates gastric cancer progression via mediating FBXL19-AS1 and miR-339-3p
Gastric cancer (GC) remains a prevalent malignancy with high morbidity and mortality. CDKN1A interacting zinc finger protein 1 (CIZ1) has been demonstrated to have oncogenic functions in the development of cancers. We detected CIZ1 expression via quantitative real-time PCR (RT-qPCR). The protein lev...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637910/ https://www.ncbi.nlm.nih.gov/pubmed/37954363 http://dx.doi.org/10.1016/j.heliyon.2023.e21061 |
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author | Wan, Houmin Wang, Lianzhen Huo, Bin Qiao, Zhongpeng Zhang, Yingli |
author_facet | Wan, Houmin Wang, Lianzhen Huo, Bin Qiao, Zhongpeng Zhang, Yingli |
author_sort | Wan, Houmin |
collection | PubMed |
description | Gastric cancer (GC) remains a prevalent malignancy with high morbidity and mortality. CDKN1A interacting zinc finger protein 1 (CIZ1) has been demonstrated to have oncogenic functions in the development of cancers. We detected CIZ1 expression via quantitative real-time PCR (RT-qPCR). The protein level of CIZ1 was measured through Western blot. We noticed that CIZ1 expression was markedly enhanced in GC cells. Furthermore, functional experiments including colony formation assay, EdU staining assay, transwell assay, TUNEL staining assay and flow cytometry analysis uncovered that CIZ1 silencing attenuated cell malignant phenotypes in GC. Through bioinformatics tools and mechanism assays, we explored the up-stream mechanism of CIZ1 and determined that CIZ1 was modulated by FBXL19 antisense RNA 1 (FBXL19-AS1) and microRNA-339-3p (miR-339-3p). Additionally, miR-339-3p exerted a negative role on GC development in vitro, and FBXL19-AS1 depletion also had the inhibitory impacts on the progression of GC in vitro. Eventually, the finding that CIZ1 overexpression reversed the effects of FBXL19-AS1 silencing on GC development was validated by rescue assays. In a word, CIZ1 functioned as a tumor promoter in GC, indicating that CIZ1 might be a promising target for GC treatment. |
format | Online Article Text |
id | pubmed-10637910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-106379102023-11-11 CIZ1 aggravates gastric cancer progression via mediating FBXL19-AS1 and miR-339-3p Wan, Houmin Wang, Lianzhen Huo, Bin Qiao, Zhongpeng Zhang, Yingli Heliyon Research Article Gastric cancer (GC) remains a prevalent malignancy with high morbidity and mortality. CDKN1A interacting zinc finger protein 1 (CIZ1) has been demonstrated to have oncogenic functions in the development of cancers. We detected CIZ1 expression via quantitative real-time PCR (RT-qPCR). The protein level of CIZ1 was measured through Western blot. We noticed that CIZ1 expression was markedly enhanced in GC cells. Furthermore, functional experiments including colony formation assay, EdU staining assay, transwell assay, TUNEL staining assay and flow cytometry analysis uncovered that CIZ1 silencing attenuated cell malignant phenotypes in GC. Through bioinformatics tools and mechanism assays, we explored the up-stream mechanism of CIZ1 and determined that CIZ1 was modulated by FBXL19 antisense RNA 1 (FBXL19-AS1) and microRNA-339-3p (miR-339-3p). Additionally, miR-339-3p exerted a negative role on GC development in vitro, and FBXL19-AS1 depletion also had the inhibitory impacts on the progression of GC in vitro. Eventually, the finding that CIZ1 overexpression reversed the effects of FBXL19-AS1 silencing on GC development was validated by rescue assays. In a word, CIZ1 functioned as a tumor promoter in GC, indicating that CIZ1 might be a promising target for GC treatment. Elsevier 2023-10-14 /pmc/articles/PMC10637910/ /pubmed/37954363 http://dx.doi.org/10.1016/j.heliyon.2023.e21061 Text en © 2023 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Wan, Houmin Wang, Lianzhen Huo, Bin Qiao, Zhongpeng Zhang, Yingli CIZ1 aggravates gastric cancer progression via mediating FBXL19-AS1 and miR-339-3p |
title | CIZ1 aggravates gastric cancer progression via mediating FBXL19-AS1 and miR-339-3p |
title_full | CIZ1 aggravates gastric cancer progression via mediating FBXL19-AS1 and miR-339-3p |
title_fullStr | CIZ1 aggravates gastric cancer progression via mediating FBXL19-AS1 and miR-339-3p |
title_full_unstemmed | CIZ1 aggravates gastric cancer progression via mediating FBXL19-AS1 and miR-339-3p |
title_short | CIZ1 aggravates gastric cancer progression via mediating FBXL19-AS1 and miR-339-3p |
title_sort | ciz1 aggravates gastric cancer progression via mediating fbxl19-as1 and mir-339-3p |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637910/ https://www.ncbi.nlm.nih.gov/pubmed/37954363 http://dx.doi.org/10.1016/j.heliyon.2023.e21061 |
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