Cargando…
Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment
Induced mutations are important for genetic research and breeding. Mutations induced by physical or chemical mutagenesis are usually heterozygous during the early generations. However, mutations must be fixed prior to phenotyping or field trials, which requires additional rounds of self-pollination....
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Nature Singapore
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10638298/ https://www.ncbi.nlm.nih.gov/pubmed/37970468 http://dx.doi.org/10.1007/s42994-023-00108-6 |
_version_ | 1785146573570703360 |
---|---|
author | Huang, Linli Gao, Guangqi Jiang, Congcong Guo, Guimei He, Qiang Zong, Yingjie Liu, Chenghong Yang, Ping |
author_facet | Huang, Linli Gao, Guangqi Jiang, Congcong Guo, Guimei He, Qiang Zong, Yingjie Liu, Chenghong Yang, Ping |
author_sort | Huang, Linli |
collection | PubMed |
description | Induced mutations are important for genetic research and breeding. Mutations induced by physical or chemical mutagenesis are usually heterozygous during the early generations. However, mutations must be fixed prior to phenotyping or field trials, which requires additional rounds of self-pollination. Microspore culture is an effective method to produce double-haploid (DH) plants that are fixed homozygotes. In this study, we conducted ethyl methanesulfonate (EMS)-induced mutagenesis of microspore cultures of barley (Hordeum vulgare) cultivar ‘Hua30’ and landrace ‘HTX’. The EMS concentrations were negatively correlated with the efficiency of callus induction and the frequency of mutant plant regeneration. The two genotypes showed different regeneration efficiencies. The phenotypic variation of the regenerated M(1) plants and the presence of genome-wide nucleotide mutations, revealed by whole-genome sequencing, highlight the utility of EMS-induced mutagenesis of isolated microspore cultures for developing DH mutants. Genome-wide analysis of the mutation frequency in the regenerated plants revealed that a considerable proportion of mutations resulted from microspore culture (somaclonal variation) rather than EMS-induced mutagenesis. In addition to producing a population of 1972 homozygous mutant lines that are available for future field trials, this study lays the foundation for optimizing the regeneration efficiency of DH plants and the richness of mutations (mainly by fine-tuning the mutagen dosage). |
format | Online Article Text |
id | pubmed-10638298 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Nature Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-106382982023-11-15 Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment Huang, Linli Gao, Guangqi Jiang, Congcong Guo, Guimei He, Qiang Zong, Yingjie Liu, Chenghong Yang, Ping aBIOTECH Research Article Induced mutations are important for genetic research and breeding. Mutations induced by physical or chemical mutagenesis are usually heterozygous during the early generations. However, mutations must be fixed prior to phenotyping or field trials, which requires additional rounds of self-pollination. Microspore culture is an effective method to produce double-haploid (DH) plants that are fixed homozygotes. In this study, we conducted ethyl methanesulfonate (EMS)-induced mutagenesis of microspore cultures of barley (Hordeum vulgare) cultivar ‘Hua30’ and landrace ‘HTX’. The EMS concentrations were negatively correlated with the efficiency of callus induction and the frequency of mutant plant regeneration. The two genotypes showed different regeneration efficiencies. The phenotypic variation of the regenerated M(1) plants and the presence of genome-wide nucleotide mutations, revealed by whole-genome sequencing, highlight the utility of EMS-induced mutagenesis of isolated microspore cultures for developing DH mutants. Genome-wide analysis of the mutation frequency in the regenerated plants revealed that a considerable proportion of mutations resulted from microspore culture (somaclonal variation) rather than EMS-induced mutagenesis. In addition to producing a population of 1972 homozygous mutant lines that are available for future field trials, this study lays the foundation for optimizing the regeneration efficiency of DH plants and the richness of mutations (mainly by fine-tuning the mutagen dosage). Springer Nature Singapore 2023-06-28 /pmc/articles/PMC10638298/ /pubmed/37970468 http://dx.doi.org/10.1007/s42994-023-00108-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Huang, Linli Gao, Guangqi Jiang, Congcong Guo, Guimei He, Qiang Zong, Yingjie Liu, Chenghong Yang, Ping Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment |
title | Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment |
title_full | Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment |
title_fullStr | Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment |
title_full_unstemmed | Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment |
title_short | Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment |
title_sort | generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10638298/ https://www.ncbi.nlm.nih.gov/pubmed/37970468 http://dx.doi.org/10.1007/s42994-023-00108-6 |
work_keys_str_mv | AT huanglinli generatinghomozygousmutantpopulationsofbarleymicrosporesbyethylmethanesulfonatetreatment AT gaoguangqi generatinghomozygousmutantpopulationsofbarleymicrosporesbyethylmethanesulfonatetreatment AT jiangcongcong generatinghomozygousmutantpopulationsofbarleymicrosporesbyethylmethanesulfonatetreatment AT guoguimei generatinghomozygousmutantpopulationsofbarleymicrosporesbyethylmethanesulfonatetreatment AT heqiang generatinghomozygousmutantpopulationsofbarleymicrosporesbyethylmethanesulfonatetreatment AT zongyingjie generatinghomozygousmutantpopulationsofbarleymicrosporesbyethylmethanesulfonatetreatment AT liuchenghong generatinghomozygousmutantpopulationsofbarleymicrosporesbyethylmethanesulfonatetreatment AT yangping generatinghomozygousmutantpopulationsofbarleymicrosporesbyethylmethanesulfonatetreatment |