Cargando…

Metatranscriptome of human lung microbial communities in a cohort of mechanically ventilated COVID-19 Omicron patients

The Omicron variant of the severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) infected a substantial proportion of Chinese population, and understanding the factors underlying the severity of the disease and fatality is valuable for future prevention and clinical treatment. We recruited 64...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Lin, Cao, Jia-Bao, Xia, Bin-Bin, Li, Yue-Juan, Zhang, Xuan, Mo, Guo-Xin, Wang, Rui-Juan, Guo, Si-Qi, Zhang, Yu-Qing, Xiao, Kun, Zhu, Guang-Fa, Liu, Peng-Fei, Song, Li-Cheng, Ma, Xi-Hui, Xiang, Ping-Chao, Wang, Jiang, Liu, Yu-Hong, Xie, Fei, Zhang, Xu-Dong, Li, Xiang-Xin, Sun, Wan-Lu, Cao, Yan, Wang, Kai-Fei, Zhang, Wen-Hui, Zhao, Wei-Chao, Yan, Peng, Chen, Ji-Chao, Yang, Yu-Wei, Yu, Zhong-Kuo, Tang, Jing-Si, Xiao, Li, Zhou, Jie-Min, Xie, Li-Xin, Wang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10638395/
https://www.ncbi.nlm.nih.gov/pubmed/37949875
http://dx.doi.org/10.1038/s41392-023-01684-1
_version_ 1785133587635372032
author Wang, Lin
Cao, Jia-Bao
Xia, Bin-Bin
Li, Yue-Juan
Zhang, Xuan
Mo, Guo-Xin
Wang, Rui-Juan
Guo, Si-Qi
Zhang, Yu-Qing
Xiao, Kun
Zhu, Guang-Fa
Liu, Peng-Fei
Song, Li-Cheng
Ma, Xi-Hui
Xiang, Ping-Chao
Wang, Jiang
Liu, Yu-Hong
Xie, Fei
Zhang, Xu-Dong
Li, Xiang-Xin
Sun, Wan-Lu
Cao, Yan
Wang, Kai-Fei
Zhang, Wen-Hui
Zhao, Wei-Chao
Yan, Peng
Chen, Ji-Chao
Yang, Yu-Wei
Yu, Zhong-Kuo
Tang, Jing-Si
Xiao, Li
Zhou, Jie-Min
Xie, Li-Xin
Wang, Jun
author_facet Wang, Lin
Cao, Jia-Bao
Xia, Bin-Bin
Li, Yue-Juan
Zhang, Xuan
Mo, Guo-Xin
Wang, Rui-Juan
Guo, Si-Qi
Zhang, Yu-Qing
Xiao, Kun
Zhu, Guang-Fa
Liu, Peng-Fei
Song, Li-Cheng
Ma, Xi-Hui
Xiang, Ping-Chao
Wang, Jiang
Liu, Yu-Hong
Xie, Fei
Zhang, Xu-Dong
Li, Xiang-Xin
Sun, Wan-Lu
Cao, Yan
Wang, Kai-Fei
Zhang, Wen-Hui
Zhao, Wei-Chao
Yan, Peng
Chen, Ji-Chao
Yang, Yu-Wei
Yu, Zhong-Kuo
Tang, Jing-Si
Xiao, Li
Zhou, Jie-Min
Xie, Li-Xin
Wang, Jun
author_sort Wang, Lin
collection PubMed
description The Omicron variant of the severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) infected a substantial proportion of Chinese population, and understanding the factors underlying the severity of the disease and fatality is valuable for future prevention and clinical treatment. We recruited 64 patients with invasive ventilation for COVID-19 and performed metatranscriptomic sequencing to profile host transcriptomic profiles, plus viral, bacterial, and fungal content, as well as virulence factors and examined their relationships to 28-day mortality were examined. In addition, the bronchoalveolar lavage fluid (BALF) samples from invasive ventilated hospital/community-acquired pneumonia patients (HAP/CAP) sampled in 2019 were included for comparison. Genomic analysis revealed that all Omicron strains belong to BA.5 and BF.7 sub-lineages, with no difference in 28-day mortality between them. Compared to HAP/CAP cohort, invasive ventilated COVID-19 patients have distinct host transcriptomic and microbial signatures in the lower respiratory tract; and in the COVID-19 non-survivors, we found significantly lower gene expressions in pathways related viral processes and positive regulation of protein localization to plasma membrane, higher abundance of opportunistic pathogens including bacterial Alloprevotella, Caulobacter, Escherichia-Shigella, Ralstonia and fungal Aspergillus sydowii and Penicillium rubens. Correlational analysis further revealed significant associations between host immune responses and microbial compositions, besides synergy within viral, bacterial, and fungal pathogens. Our study presents the relationships of lower respiratory tract microbiome and transcriptome in invasive ventilated COVID-19 patients, providing the basis for future clinical treatment and reduction of fatality.
format Online
Article
Text
id pubmed-10638395
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-106383952023-11-11 Metatranscriptome of human lung microbial communities in a cohort of mechanically ventilated COVID-19 Omicron patients Wang, Lin Cao, Jia-Bao Xia, Bin-Bin Li, Yue-Juan Zhang, Xuan Mo, Guo-Xin Wang, Rui-Juan Guo, Si-Qi Zhang, Yu-Qing Xiao, Kun Zhu, Guang-Fa Liu, Peng-Fei Song, Li-Cheng Ma, Xi-Hui Xiang, Ping-Chao Wang, Jiang Liu, Yu-Hong Xie, Fei Zhang, Xu-Dong Li, Xiang-Xin Sun, Wan-Lu Cao, Yan Wang, Kai-Fei Zhang, Wen-Hui Zhao, Wei-Chao Yan, Peng Chen, Ji-Chao Yang, Yu-Wei Yu, Zhong-Kuo Tang, Jing-Si Xiao, Li Zhou, Jie-Min Xie, Li-Xin Wang, Jun Signal Transduct Target Ther Article The Omicron variant of the severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) infected a substantial proportion of Chinese population, and understanding the factors underlying the severity of the disease and fatality is valuable for future prevention and clinical treatment. We recruited 64 patients with invasive ventilation for COVID-19 and performed metatranscriptomic sequencing to profile host transcriptomic profiles, plus viral, bacterial, and fungal content, as well as virulence factors and examined their relationships to 28-day mortality were examined. In addition, the bronchoalveolar lavage fluid (BALF) samples from invasive ventilated hospital/community-acquired pneumonia patients (HAP/CAP) sampled in 2019 were included for comparison. Genomic analysis revealed that all Omicron strains belong to BA.5 and BF.7 sub-lineages, with no difference in 28-day mortality between them. Compared to HAP/CAP cohort, invasive ventilated COVID-19 patients have distinct host transcriptomic and microbial signatures in the lower respiratory tract; and in the COVID-19 non-survivors, we found significantly lower gene expressions in pathways related viral processes and positive regulation of protein localization to plasma membrane, higher abundance of opportunistic pathogens including bacterial Alloprevotella, Caulobacter, Escherichia-Shigella, Ralstonia and fungal Aspergillus sydowii and Penicillium rubens. Correlational analysis further revealed significant associations between host immune responses and microbial compositions, besides synergy within viral, bacterial, and fungal pathogens. Our study presents the relationships of lower respiratory tract microbiome and transcriptome in invasive ventilated COVID-19 patients, providing the basis for future clinical treatment and reduction of fatality. Nature Publishing Group UK 2023-11-10 /pmc/articles/PMC10638395/ /pubmed/37949875 http://dx.doi.org/10.1038/s41392-023-01684-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wang, Lin
Cao, Jia-Bao
Xia, Bin-Bin
Li, Yue-Juan
Zhang, Xuan
Mo, Guo-Xin
Wang, Rui-Juan
Guo, Si-Qi
Zhang, Yu-Qing
Xiao, Kun
Zhu, Guang-Fa
Liu, Peng-Fei
Song, Li-Cheng
Ma, Xi-Hui
Xiang, Ping-Chao
Wang, Jiang
Liu, Yu-Hong
Xie, Fei
Zhang, Xu-Dong
Li, Xiang-Xin
Sun, Wan-Lu
Cao, Yan
Wang, Kai-Fei
Zhang, Wen-Hui
Zhao, Wei-Chao
Yan, Peng
Chen, Ji-Chao
Yang, Yu-Wei
Yu, Zhong-Kuo
Tang, Jing-Si
Xiao, Li
Zhou, Jie-Min
Xie, Li-Xin
Wang, Jun
Metatranscriptome of human lung microbial communities in a cohort of mechanically ventilated COVID-19 Omicron patients
title Metatranscriptome of human lung microbial communities in a cohort of mechanically ventilated COVID-19 Omicron patients
title_full Metatranscriptome of human lung microbial communities in a cohort of mechanically ventilated COVID-19 Omicron patients
title_fullStr Metatranscriptome of human lung microbial communities in a cohort of mechanically ventilated COVID-19 Omicron patients
title_full_unstemmed Metatranscriptome of human lung microbial communities in a cohort of mechanically ventilated COVID-19 Omicron patients
title_short Metatranscriptome of human lung microbial communities in a cohort of mechanically ventilated COVID-19 Omicron patients
title_sort metatranscriptome of human lung microbial communities in a cohort of mechanically ventilated covid-19 omicron patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10638395/
https://www.ncbi.nlm.nih.gov/pubmed/37949875
http://dx.doi.org/10.1038/s41392-023-01684-1
work_keys_str_mv AT wanglin metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT caojiabao metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT xiabinbin metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT liyuejuan metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT zhangxuan metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT moguoxin metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT wangruijuan metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT guosiqi metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT zhangyuqing metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT xiaokun metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT zhuguangfa metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT liupengfei metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT songlicheng metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT maxihui metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT xiangpingchao metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT wangjiang metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT liuyuhong metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT xiefei metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT zhangxudong metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT lixiangxin metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT sunwanlu metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT caoyan metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT wangkaifei metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT zhangwenhui metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT zhaoweichao metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT yanpeng metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT chenjichao metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT yangyuwei metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT yuzhongkuo metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT tangjingsi metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT xiaoli metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT zhoujiemin metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT xielixin metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients
AT wangjun metatranscriptomeofhumanlungmicrobialcommunitiesinacohortofmechanicallyventilatedcovid19omicronpatients