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In vivo-like nearest neighbor parameters improve prediction of fractional RNA base-pairing in cells
We conducted a thermodynamic analysis of RNA stability in Eco80 artificial cytoplasm, which mimics in vivo conditions, and compared it to transcriptome-wide probing of mRNA. Eco80 contains 80% of Escherichia coli metabolites, with biological concentrations of metal ions, including 2 mM free Mg(2+) a...
Autores principales: | Sieg, Jacob P, Jolley, Elizabeth A, Huot, Melanie J, Babitzke, Paul, Bevilacqua, Philip C |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10639048/ https://www.ncbi.nlm.nih.gov/pubmed/37855684 http://dx.doi.org/10.1093/nar/gkad807 |
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