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In vivo-like nearest neighbor parameters improve prediction of fractional RNA base-pairing in cells

We conducted a thermodynamic analysis of RNA stability in Eco80 artificial cytoplasm, which mimics in vivo conditions, and compared it to transcriptome-wide probing of mRNA. Eco80 contains 80% of Escherichia coli metabolites, with biological concentrations of metal ions, including 2 mM free Mg(2+) a...

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Detalles Bibliográficos
Autores principales:	Sieg, Jacob P, Jolley, Elizabeth A, Huot, Melanie J, Babitzke, Paul, Bevilacqua, Philip C
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2023
Materias:	RNA and RNA-protein complexes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10639048/ https://www.ncbi.nlm.nih.gov/pubmed/37855684 http://dx.doi.org/10.1093/nar/gkad807

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