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Inhibition of urethral stricture by a catheter loaded with nanoparticle/ pirfenidone complexes

Background: Urethral strictures are common injurious conditions of the urinary system. Reducing and preventing urethral strictures has become a hot and challenging topic for urological surgeons and related researchers. In this study, we developed a catheter loaded with nanoparticle/pirfenidone (NP/P...

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Autores principales: Meng, Wei, Jiang, Zhaosheng, Wang, Jiahao, Chen, Xiaohua, Chen, Bo, Cai, Bo, Zhou, Youlang, Ma, Limin, Guan, Yangbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10639154/
https://www.ncbi.nlm.nih.gov/pubmed/37954024
http://dx.doi.org/10.3389/fbioe.2023.1254621
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author Meng, Wei
Jiang, Zhaosheng
Wang, Jiahao
Chen, Xiaohua
Chen, Bo
Cai, Bo
Zhou, Youlang
Ma, Limin
Guan, Yangbo
author_facet Meng, Wei
Jiang, Zhaosheng
Wang, Jiahao
Chen, Xiaohua
Chen, Bo
Cai, Bo
Zhou, Youlang
Ma, Limin
Guan, Yangbo
author_sort Meng, Wei
collection PubMed
description Background: Urethral strictures are common injurious conditions of the urinary system. Reducing and preventing urethral strictures has become a hot and challenging topic for urological surgeons and related researchers. In this study, we developed a catheter loaded with nanoparticle/pirfenidone (NP/PFD) complexes and evaluated its effectiveness at inhibiting urethral stricture in rabbits, providing more references for the clinical prevention and reduction of urethral stenosis. Methods: Twelve adult male New Zealand rabbits were selected and divided into the following four groups in a ratio of 1:1:1:1 using the random number table method: Group A, sham; Group B, urethral stricture (US); Group C, US + unmodified catheter; and Group D, US + NP/PFD catheter. On the 30th day after modelling, retrograde urethrography was performed to evaluate urethral stricture formation, and histopathological examination was performed on the tissues of the corresponding surgical site. Meanwhile, changes in the expression level of Transforming growth factor β1 (TGF-β1) in the tissues were detected by immunohistochemistry. Results: The NP/PFD complexes adhered uniformly to the catheter surface. They remained on the surface of the catheter after insertion into the urethra. In addition, the NP/PFD complexes spread into the urethral epithelium 2 weeks after surgery. Ultimately, urethral strictures were significantly reduced with the placement of the NP/PFD complex catheter. Conclusion: Our catheter loaded with NP/PFD complexes effectively delivered PFD to the urethral epithelium through continuous local delivery, thereby reducing fibrosis and stricture after urethral injury, which may be associated with the inhibition of TGF-β1 expression.
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spelling pubmed-106391542023-11-11 Inhibition of urethral stricture by a catheter loaded with nanoparticle/ pirfenidone complexes Meng, Wei Jiang, Zhaosheng Wang, Jiahao Chen, Xiaohua Chen, Bo Cai, Bo Zhou, Youlang Ma, Limin Guan, Yangbo Front Bioeng Biotechnol Bioengineering and Biotechnology Background: Urethral strictures are common injurious conditions of the urinary system. Reducing and preventing urethral strictures has become a hot and challenging topic for urological surgeons and related researchers. In this study, we developed a catheter loaded with nanoparticle/pirfenidone (NP/PFD) complexes and evaluated its effectiveness at inhibiting urethral stricture in rabbits, providing more references for the clinical prevention and reduction of urethral stenosis. Methods: Twelve adult male New Zealand rabbits were selected and divided into the following four groups in a ratio of 1:1:1:1 using the random number table method: Group A, sham; Group B, urethral stricture (US); Group C, US + unmodified catheter; and Group D, US + NP/PFD catheter. On the 30th day after modelling, retrograde urethrography was performed to evaluate urethral stricture formation, and histopathological examination was performed on the tissues of the corresponding surgical site. Meanwhile, changes in the expression level of Transforming growth factor β1 (TGF-β1) in the tissues were detected by immunohistochemistry. Results: The NP/PFD complexes adhered uniformly to the catheter surface. They remained on the surface of the catheter after insertion into the urethra. In addition, the NP/PFD complexes spread into the urethral epithelium 2 weeks after surgery. Ultimately, urethral strictures were significantly reduced with the placement of the NP/PFD complex catheter. Conclusion: Our catheter loaded with NP/PFD complexes effectively delivered PFD to the urethral epithelium through continuous local delivery, thereby reducing fibrosis and stricture after urethral injury, which may be associated with the inhibition of TGF-β1 expression. Frontiers Media S.A. 2023-10-26 /pmc/articles/PMC10639154/ /pubmed/37954024 http://dx.doi.org/10.3389/fbioe.2023.1254621 Text en Copyright © 2023 Meng, Jiang, Wang, Chen, Chen, Cai, Zhou, Ma and Guan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Meng, Wei
Jiang, Zhaosheng
Wang, Jiahao
Chen, Xiaohua
Chen, Bo
Cai, Bo
Zhou, Youlang
Ma, Limin
Guan, Yangbo
Inhibition of urethral stricture by a catheter loaded with nanoparticle/ pirfenidone complexes
title Inhibition of urethral stricture by a catheter loaded with nanoparticle/ pirfenidone complexes
title_full Inhibition of urethral stricture by a catheter loaded with nanoparticle/ pirfenidone complexes
title_fullStr Inhibition of urethral stricture by a catheter loaded with nanoparticle/ pirfenidone complexes
title_full_unstemmed Inhibition of urethral stricture by a catheter loaded with nanoparticle/ pirfenidone complexes
title_short Inhibition of urethral stricture by a catheter loaded with nanoparticle/ pirfenidone complexes
title_sort inhibition of urethral stricture by a catheter loaded with nanoparticle/ pirfenidone complexes
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10639154/
https://www.ncbi.nlm.nih.gov/pubmed/37954024
http://dx.doi.org/10.3389/fbioe.2023.1254621
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