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Skeletal muscle cells opto-stimulation by intramembrane molecular transducers
Optical stimulation and control of muscle cell contraction opens up a number of interesting applications in hybrid robotic and medicine. Here we show that recently designed molecular phototransducer can be used to stimulate C2C12 skeletal muscle cells, properly grown to exhibit collective behaviour....
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10640616/ https://www.ncbi.nlm.nih.gov/pubmed/37952040 http://dx.doi.org/10.1038/s42003-023-05538-y |
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author | Venturino, Ilaria Vurro, Vito Bonfadini, Silvio Moschetta, Matteo Perotto, Sara Sesti, Valentina Criante, Luigino Bertarelli, Chiara Lanzani, Guglielmo |
author_facet | Venturino, Ilaria Vurro, Vito Bonfadini, Silvio Moschetta, Matteo Perotto, Sara Sesti, Valentina Criante, Luigino Bertarelli, Chiara Lanzani, Guglielmo |
author_sort | Venturino, Ilaria |
collection | PubMed |
description | Optical stimulation and control of muscle cell contraction opens up a number of interesting applications in hybrid robotic and medicine. Here we show that recently designed molecular phototransducer can be used to stimulate C2C12 skeletal muscle cells, properly grown to exhibit collective behaviour. C2C12 is a skeletal muscle cell line that does not require animal sacrifice Furthermore, it is an ideal cell model for evaluating the phototransducer pacing ability due to its negligible spontaneous activity. We study the stimulation process and analyse the distribution of responses in multinuclear cells, in particular looking at the consistency between stimulus and contraction. Contractions are detected by using an imaging software for object recognition. We find a deterministic response to light stimuli, yet with a certain distribution of erratic behaviour that is quantified and correlated to light intensity or stimulation frequency. Finally, we compare our optical stimulation with electrical stimulation showing advantages of the optical approach, like the reduced cell stress. |
format | Online Article Text |
id | pubmed-10640616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106406162023-11-11 Skeletal muscle cells opto-stimulation by intramembrane molecular transducers Venturino, Ilaria Vurro, Vito Bonfadini, Silvio Moschetta, Matteo Perotto, Sara Sesti, Valentina Criante, Luigino Bertarelli, Chiara Lanzani, Guglielmo Commun Biol Article Optical stimulation and control of muscle cell contraction opens up a number of interesting applications in hybrid robotic and medicine. Here we show that recently designed molecular phototransducer can be used to stimulate C2C12 skeletal muscle cells, properly grown to exhibit collective behaviour. C2C12 is a skeletal muscle cell line that does not require animal sacrifice Furthermore, it is an ideal cell model for evaluating the phototransducer pacing ability due to its negligible spontaneous activity. We study the stimulation process and analyse the distribution of responses in multinuclear cells, in particular looking at the consistency between stimulus and contraction. Contractions are detected by using an imaging software for object recognition. We find a deterministic response to light stimuli, yet with a certain distribution of erratic behaviour that is quantified and correlated to light intensity or stimulation frequency. Finally, we compare our optical stimulation with electrical stimulation showing advantages of the optical approach, like the reduced cell stress. Nature Publishing Group UK 2023-11-11 /pmc/articles/PMC10640616/ /pubmed/37952040 http://dx.doi.org/10.1038/s42003-023-05538-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Venturino, Ilaria Vurro, Vito Bonfadini, Silvio Moschetta, Matteo Perotto, Sara Sesti, Valentina Criante, Luigino Bertarelli, Chiara Lanzani, Guglielmo Skeletal muscle cells opto-stimulation by intramembrane molecular transducers |
title | Skeletal muscle cells opto-stimulation by intramembrane molecular transducers |
title_full | Skeletal muscle cells opto-stimulation by intramembrane molecular transducers |
title_fullStr | Skeletal muscle cells opto-stimulation by intramembrane molecular transducers |
title_full_unstemmed | Skeletal muscle cells opto-stimulation by intramembrane molecular transducers |
title_short | Skeletal muscle cells opto-stimulation by intramembrane molecular transducers |
title_sort | skeletal muscle cells opto-stimulation by intramembrane molecular transducers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10640616/ https://www.ncbi.nlm.nih.gov/pubmed/37952040 http://dx.doi.org/10.1038/s42003-023-05538-y |
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