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Insights into the influence of physicochemical parameters on the microbial community and volatile compounds during the ultra-long fermentation of compound-flavor Baijiu

INTRODUCTION: While the variation in physicochemical parameters, microbial communities, metabolism, composition, and the proportion of volatile components in fermented grains (FG) affect final Baijiu quality, their complex interactions during the ultra-long fermentation of compound-flavor Baijiu (CF...

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Autores principales: Cheng, Wei, Chen, Xuefeng, Lan, Wei, Liu, Gengdian, Xue, Xijia, Li, Ruilong, Pan, Tianquan, Li, Na, Zhou, Duan, Chen, Xingjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641013/
https://www.ncbi.nlm.nih.gov/pubmed/37965554
http://dx.doi.org/10.3389/fmicb.2023.1272559
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author Cheng, Wei
Chen, Xuefeng
Lan, Wei
Liu, Gengdian
Xue, Xijia
Li, Ruilong
Pan, Tianquan
Li, Na
Zhou, Duan
Chen, Xingjie
author_facet Cheng, Wei
Chen, Xuefeng
Lan, Wei
Liu, Gengdian
Xue, Xijia
Li, Ruilong
Pan, Tianquan
Li, Na
Zhou, Duan
Chen, Xingjie
author_sort Cheng, Wei
collection PubMed
description INTRODUCTION: While the variation in physicochemical parameters, microbial communities, metabolism, composition, and the proportion of volatile components in fermented grains (FG) affect final Baijiu quality, their complex interactions during the ultra-long fermentation of compound-flavor Baijiu (CFB) are still poorly understood. METHODS: In this study, amplicon sequencing was used to analyze the microbial community, and headspace solid-phase microextraction-gas chromatography–mass spectrometry (HS-SPME-GC–MS) was used to analyze the volatile components in FG during ultra-long fermentation of CFB. The relationships between the dominant microbial communities, physicochemical parameters, and volatile components were analyzed using redundancy analysis and network analysis. RESULTS: During ultra-long fermentation, bacterial diversity was initially higher than during the mid and late stages. Fungal diversity in the mid stages was higher than that initially and later in the process. A total of 88 volatile components, including six alcohols, 43 esters, eight aldehydes and ketones, 13 acids, and 18 other compounds were detected in FG. Starch and reducing sugars in FG strongly affected the composition and function of bacterial and fungal communities. However, acidity had little effect on the composition and function of the bacterial flora. Lactobacillus, Bacillus, Weissella, and Pichia were the core microbial genera involved in metabolizing the volatile components of FG. DISCUSSION: We provide insights into the relationships and influences among the dominant microbial communities, physicochemical parameters, and volatile components during ultra-long fermentation of CFB. These insights help clarify the fermentation mechanisms of solid-state fermentation Baijiu (SFB) and control and improve the aroma quality of CFB.
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spelling pubmed-106410132023-11-14 Insights into the influence of physicochemical parameters on the microbial community and volatile compounds during the ultra-long fermentation of compound-flavor Baijiu Cheng, Wei Chen, Xuefeng Lan, Wei Liu, Gengdian Xue, Xijia Li, Ruilong Pan, Tianquan Li, Na Zhou, Duan Chen, Xingjie Front Microbiol Microbiology INTRODUCTION: While the variation in physicochemical parameters, microbial communities, metabolism, composition, and the proportion of volatile components in fermented grains (FG) affect final Baijiu quality, their complex interactions during the ultra-long fermentation of compound-flavor Baijiu (CFB) are still poorly understood. METHODS: In this study, amplicon sequencing was used to analyze the microbial community, and headspace solid-phase microextraction-gas chromatography–mass spectrometry (HS-SPME-GC–MS) was used to analyze the volatile components in FG during ultra-long fermentation of CFB. The relationships between the dominant microbial communities, physicochemical parameters, and volatile components were analyzed using redundancy analysis and network analysis. RESULTS: During ultra-long fermentation, bacterial diversity was initially higher than during the mid and late stages. Fungal diversity in the mid stages was higher than that initially and later in the process. A total of 88 volatile components, including six alcohols, 43 esters, eight aldehydes and ketones, 13 acids, and 18 other compounds were detected in FG. Starch and reducing sugars in FG strongly affected the composition and function of bacterial and fungal communities. However, acidity had little effect on the composition and function of the bacterial flora. Lactobacillus, Bacillus, Weissella, and Pichia were the core microbial genera involved in metabolizing the volatile components of FG. DISCUSSION: We provide insights into the relationships and influences among the dominant microbial communities, physicochemical parameters, and volatile components during ultra-long fermentation of CFB. These insights help clarify the fermentation mechanisms of solid-state fermentation Baijiu (SFB) and control and improve the aroma quality of CFB. Frontiers Media S.A. 2023-10-26 /pmc/articles/PMC10641013/ /pubmed/37965554 http://dx.doi.org/10.3389/fmicb.2023.1272559 Text en Copyright © 2023 Cheng, Chen, Lan, Liu, Xue, Li, Pan, Li, Zhou and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Cheng, Wei
Chen, Xuefeng
Lan, Wei
Liu, Gengdian
Xue, Xijia
Li, Ruilong
Pan, Tianquan
Li, Na
Zhou, Duan
Chen, Xingjie
Insights into the influence of physicochemical parameters on the microbial community and volatile compounds during the ultra-long fermentation of compound-flavor Baijiu
title Insights into the influence of physicochemical parameters on the microbial community and volatile compounds during the ultra-long fermentation of compound-flavor Baijiu
title_full Insights into the influence of physicochemical parameters on the microbial community and volatile compounds during the ultra-long fermentation of compound-flavor Baijiu
title_fullStr Insights into the influence of physicochemical parameters on the microbial community and volatile compounds during the ultra-long fermentation of compound-flavor Baijiu
title_full_unstemmed Insights into the influence of physicochemical parameters on the microbial community and volatile compounds during the ultra-long fermentation of compound-flavor Baijiu
title_short Insights into the influence of physicochemical parameters on the microbial community and volatile compounds during the ultra-long fermentation of compound-flavor Baijiu
title_sort insights into the influence of physicochemical parameters on the microbial community and volatile compounds during the ultra-long fermentation of compound-flavor baijiu
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641013/
https://www.ncbi.nlm.nih.gov/pubmed/37965554
http://dx.doi.org/10.3389/fmicb.2023.1272559
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