Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas

Diabetes mellitus is a disease of the hormone-secreting endocrine pancreas. However, increasing evidence suggests that the exocrine pancreas is also involved in the pathogenesis of diabetes. In this protocol, we describe how to harvest both isolated islets and exocrine tissue from one mouse pancreas...

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Autores principales: Steiger, Laura, Baumann, Zora, Keller, Lena, Böni-Schnetzler, Marianne, Donath, Marc Y., Meier, Daniel T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641308/
https://www.ncbi.nlm.nih.gov/pubmed/37889759
http://dx.doi.org/10.1016/j.xpro.2023.102664
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author Steiger, Laura
Baumann, Zora
Keller, Lena
Böni-Schnetzler, Marianne
Donath, Marc Y.
Meier, Daniel T.
author_facet Steiger, Laura
Baumann, Zora
Keller, Lena
Böni-Schnetzler, Marianne
Donath, Marc Y.
Meier, Daniel T.
author_sort Steiger, Laura
collection PubMed
description Diabetes mellitus is a disease of the hormone-secreting endocrine pancreas. However, increasing evidence suggests that the exocrine pancreas is also involved in the pathogenesis of diabetes. In this protocol, we describe how to harvest both isolated islets and exocrine tissue from one mouse pancreas, followed by a detailed explanation of how to isolate and analyze immune cells using full-spectrum flow cytometry.
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spelling pubmed-106413082023-11-14 Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas Steiger, Laura Baumann, Zora Keller, Lena Böni-Schnetzler, Marianne Donath, Marc Y. Meier, Daniel T. STAR Protoc Protocol Diabetes mellitus is a disease of the hormone-secreting endocrine pancreas. However, increasing evidence suggests that the exocrine pancreas is also involved in the pathogenesis of diabetes. In this protocol, we describe how to harvest both isolated islets and exocrine tissue from one mouse pancreas, followed by a detailed explanation of how to isolate and analyze immune cells using full-spectrum flow cytometry. Elsevier 2023-10-27 /pmc/articles/PMC10641308/ /pubmed/37889759 http://dx.doi.org/10.1016/j.xpro.2023.102664 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Steiger, Laura
Baumann, Zora
Keller, Lena
Böni-Schnetzler, Marianne
Donath, Marc Y.
Meier, Daniel T.
Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas
title Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas
title_full Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas
title_fullStr Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas
title_full_unstemmed Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas
title_short Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas
title_sort protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641308/
https://www.ncbi.nlm.nih.gov/pubmed/37889759
http://dx.doi.org/10.1016/j.xpro.2023.102664
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