Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy

Glycan-glycan interactions between viral particles and host cells may lengthen the dwell time of the virus on the cell surface to facilitate cellular receptor engagement. Here, we present a protocol for visualizing glycan-mediated binding between virus or virus-like-particles (VLPs) and human periph...

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Autores principales: Spillings, Belinda L., Green, Kathryn, Lovas, Erica, Webb, Richard, Mak, Johnson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641310/
https://www.ncbi.nlm.nih.gov/pubmed/37889755
http://dx.doi.org/10.1016/j.xpro.2023.102673
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author Spillings, Belinda L.
Green, Kathryn
Lovas, Erica
Webb, Richard
Mak, Johnson
author_facet Spillings, Belinda L.
Green, Kathryn
Lovas, Erica
Webb, Richard
Mak, Johnson
author_sort Spillings, Belinda L.
collection PubMed
description Glycan-glycan interactions between viral particles and host cells may lengthen the dwell time of the virus on the cell surface to facilitate cellular receptor engagement. Here, we present a protocol for visualizing glycan-mediated binding between virus or virus-like-particles (VLPs) and human peripheral blood mononuclear cells using transmission electron microscopy (TEM). We describe steps for virus and VLP production, isolation of human peripheral blood mononuclear cells, and sample preparation. We then detail procedures for thin-section TEM. For complete details on the use and execution of this protocol, please refer to Spillings et al.(1)
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spelling pubmed-106413102023-11-14 Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy Spillings, Belinda L. Green, Kathryn Lovas, Erica Webb, Richard Mak, Johnson STAR Protoc Protocol Glycan-glycan interactions between viral particles and host cells may lengthen the dwell time of the virus on the cell surface to facilitate cellular receptor engagement. Here, we present a protocol for visualizing glycan-mediated binding between virus or virus-like-particles (VLPs) and human peripheral blood mononuclear cells using transmission electron microscopy (TEM). We describe steps for virus and VLP production, isolation of human peripheral blood mononuclear cells, and sample preparation. We then detail procedures for thin-section TEM. For complete details on the use and execution of this protocol, please refer to Spillings et al.(1) Elsevier 2023-10-27 /pmc/articles/PMC10641310/ /pubmed/37889755 http://dx.doi.org/10.1016/j.xpro.2023.102673 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Spillings, Belinda L.
Green, Kathryn
Lovas, Erica
Webb, Richard
Mak, Johnson
Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy
title Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy
title_full Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy
title_fullStr Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy
title_full_unstemmed Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy
title_short Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy
title_sort protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641310/
https://www.ncbi.nlm.nih.gov/pubmed/37889755
http://dx.doi.org/10.1016/j.xpro.2023.102673
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