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Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorectal cancer patients

Aberrant methylation of DNA has been shown to play an important role in a variety of human cancers, developmental disorders and aging. Hence, aberrant methylation patterns in genes can be a molecular marker for such conditions. Therefore, a reliable but uncomplicated method to detect DNA methylation...

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Detalles Bibliográficos
Autores principales: Kimura, Naoki, Nagasaka, Takeshi, Murakami, Jun, Sasamoto, Hiromi, Murakami, Masahiro, Tanaka, Noriaki, Matsubara, Nagahide
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1064143/
https://www.ncbi.nlm.nih.gov/pubmed/15760842
http://dx.doi.org/10.1093/nar/gni046
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author Kimura, Naoki
Nagasaka, Takeshi
Murakami, Jun
Sasamoto, Hiromi
Murakami, Masahiro
Tanaka, Noriaki
Matsubara, Nagahide
author_facet Kimura, Naoki
Nagasaka, Takeshi
Murakami, Jun
Sasamoto, Hiromi
Murakami, Masahiro
Tanaka, Noriaki
Matsubara, Nagahide
author_sort Kimura, Naoki
collection PubMed
description Aberrant methylation of DNA has been shown to play an important role in a variety of human cancers, developmental disorders and aging. Hence, aberrant methylation patterns in genes can be a molecular marker for such conditions. Therefore, a reliable but uncomplicated method to detect DNA methylation is preferred, not merely for research purposes but for daily clinical practice. To achieve these aims, we have established a precise system to identify DNA methylation patterns based on an oligonucleotide microarray technology. Our microarray method has an advantage over conventional methods and is unique because it allows the precise measurement of the methylation patterns within a target region. Our simple signal detection system depends on using an avidin–biotinylated peroxidase complex and does not require an expensive laser scanner or hazardous radioisotope. In this study, we applied our technique to detect promoter methylation status of O(6)-methylguanine-DNA methyltransferase (MGMT) gene. Our easy-handling technology provided reproducible and precise measurement of methylated CpGs in MGMT promoter and, thus, our method may bring about a potential evolution in the handling of a variety of high-throughput DNA methylation analyses for clinical purposes.
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spelling pubmed-10641432005-03-10 Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorectal cancer patients Kimura, Naoki Nagasaka, Takeshi Murakami, Jun Sasamoto, Hiromi Murakami, Masahiro Tanaka, Noriaki Matsubara, Nagahide Nucleic Acids Res Methods Online Aberrant methylation of DNA has been shown to play an important role in a variety of human cancers, developmental disorders and aging. Hence, aberrant methylation patterns in genes can be a molecular marker for such conditions. Therefore, a reliable but uncomplicated method to detect DNA methylation is preferred, not merely for research purposes but for daily clinical practice. To achieve these aims, we have established a precise system to identify DNA methylation patterns based on an oligonucleotide microarray technology. Our microarray method has an advantage over conventional methods and is unique because it allows the precise measurement of the methylation patterns within a target region. Our simple signal detection system depends on using an avidin–biotinylated peroxidase complex and does not require an expensive laser scanner or hazardous radioisotope. In this study, we applied our technique to detect promoter methylation status of O(6)-methylguanine-DNA methyltransferase (MGMT) gene. Our easy-handling technology provided reproducible and precise measurement of methylated CpGs in MGMT promoter and, thus, our method may bring about a potential evolution in the handling of a variety of high-throughput DNA methylation analyses for clinical purposes. Oxford University Press 2005 2005-03-10 /pmc/articles/PMC1064143/ /pubmed/15760842 http://dx.doi.org/10.1093/nar/gni046 Text en © The Author 2005. Published by Oxford University Press. All rights reserved
spellingShingle Methods Online
Kimura, Naoki
Nagasaka, Takeshi
Murakami, Jun
Sasamoto, Hiromi
Murakami, Masahiro
Tanaka, Noriaki
Matsubara, Nagahide
Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorectal cancer patients
title Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorectal cancer patients
title_full Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorectal cancer patients
title_fullStr Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorectal cancer patients
title_full_unstemmed Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorectal cancer patients
title_short Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorectal cancer patients
title_sort methylation profiles of genes utilizing newly developed cpg island methylation microarray on colorectal cancer patients
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1064143/
https://www.ncbi.nlm.nih.gov/pubmed/15760842
http://dx.doi.org/10.1093/nar/gni046
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