Cargando…

Global expression patterns of R-genes in tomato and potato

INTRODUCTION: As key-players of plant immunity, the proteins encoded by resistance genes (R-genes) recognize pathogens and initiate pathogen-specific defense responses. The expression of some R-genes carry fitness costs and therefore inducible immune responses are likely advantageous. To what degree...

Descripción completa

Detalles Bibliográficos
Autores principales: von Dahlen, Janina K., Schulz, Kerstin, Nicolai, Jessica, Rose, Laura E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641715/
https://www.ncbi.nlm.nih.gov/pubmed/37965025
http://dx.doi.org/10.3389/fpls.2023.1216795
_version_ 1785146815832653824
author von Dahlen, Janina K.
Schulz, Kerstin
Nicolai, Jessica
Rose, Laura E.
author_facet von Dahlen, Janina K.
Schulz, Kerstin
Nicolai, Jessica
Rose, Laura E.
author_sort von Dahlen, Janina K.
collection PubMed
description INTRODUCTION: As key-players of plant immunity, the proteins encoded by resistance genes (R-genes) recognize pathogens and initiate pathogen-specific defense responses. The expression of some R-genes carry fitness costs and therefore inducible immune responses are likely advantageous. To what degree inducible resistance driven by R-genes is triggered by pathogen infection is currently an open question. METHODS: In this study we analyzed the expression of 940 R-genes of tomato and potato across 315 transcriptome libraries to investigate how interspecific interactions with microbes influence R-gene expression in plants. RESULTS: We found that most R-genes are expressed at a low level. A small subset of R-genes had moderate to high levels of expression and were expressed across many independent libraries, irrespective of infection status. These R-genes include members of the class of genes called NRCs (NLR required for cell death). Approximately 10% of all R-genes were differentially expressed during infection and this included both up- and down-regulation. One factor associated with the large differences in R-gene expression was host tissue, reflecting a considerable degree of tissue-specific transcriptional regulation of this class of genes. DISCUSSION: These results call into question the widespread view that R-gene expression is induced upon pathogen attack. Instead, a small core set of R-genes is constitutively expressed, imparting upon the plant a ready-to-detect and defend status.
format Online
Article
Text
id pubmed-10641715
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-106417152023-11-14 Global expression patterns of R-genes in tomato and potato von Dahlen, Janina K. Schulz, Kerstin Nicolai, Jessica Rose, Laura E. Front Plant Sci Plant Science INTRODUCTION: As key-players of plant immunity, the proteins encoded by resistance genes (R-genes) recognize pathogens and initiate pathogen-specific defense responses. The expression of some R-genes carry fitness costs and therefore inducible immune responses are likely advantageous. To what degree inducible resistance driven by R-genes is triggered by pathogen infection is currently an open question. METHODS: In this study we analyzed the expression of 940 R-genes of tomato and potato across 315 transcriptome libraries to investigate how interspecific interactions with microbes influence R-gene expression in plants. RESULTS: We found that most R-genes are expressed at a low level. A small subset of R-genes had moderate to high levels of expression and were expressed across many independent libraries, irrespective of infection status. These R-genes include members of the class of genes called NRCs (NLR required for cell death). Approximately 10% of all R-genes were differentially expressed during infection and this included both up- and down-regulation. One factor associated with the large differences in R-gene expression was host tissue, reflecting a considerable degree of tissue-specific transcriptional regulation of this class of genes. DISCUSSION: These results call into question the widespread view that R-gene expression is induced upon pathogen attack. Instead, a small core set of R-genes is constitutively expressed, imparting upon the plant a ready-to-detect and defend status. Frontiers Media S.A. 2023-10-27 /pmc/articles/PMC10641715/ /pubmed/37965025 http://dx.doi.org/10.3389/fpls.2023.1216795 Text en Copyright © 2023 von Dahlen, Schulz, Nicolai and Rose https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
von Dahlen, Janina K.
Schulz, Kerstin
Nicolai, Jessica
Rose, Laura E.
Global expression patterns of R-genes in tomato and potato
title Global expression patterns of R-genes in tomato and potato
title_full Global expression patterns of R-genes in tomato and potato
title_fullStr Global expression patterns of R-genes in tomato and potato
title_full_unstemmed Global expression patterns of R-genes in tomato and potato
title_short Global expression patterns of R-genes in tomato and potato
title_sort global expression patterns of r-genes in tomato and potato
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641715/
https://www.ncbi.nlm.nih.gov/pubmed/37965025
http://dx.doi.org/10.3389/fpls.2023.1216795
work_keys_str_mv AT vondahlenjaninak globalexpressionpatternsofrgenesintomatoandpotato
AT schulzkerstin globalexpressionpatternsofrgenesintomatoandpotato
AT nicolaijessica globalexpressionpatternsofrgenesintomatoandpotato
AT roselaurae globalexpressionpatternsofrgenesintomatoandpotato