Cargando…

Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency

This study aimed to develop a long-term pollen storage protocol for Luffa species (L. acutangula, L. cylindrica, L. echinata, and L. graveolens) and assess its potential for crop improvement. The optimal medium for in vitro pollen germination varied by species, with Brewbaker and Kwack (BK) medium w...

Descripción completa

Detalles Bibliográficos
Autores principales: Nagar, Arvind, Gowthami, Ravi, Sureja, Amish Kumar, Munshi, Anilabha Das, Verma, Manjusha, Singh, Awani Kumar, Mallick, Niharika, Singh, Jogendra, Chander, Subhash, Shankar, Muthusamy, Pathania, Pooja, Rajkumar, Subramani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641756/
https://www.ncbi.nlm.nih.gov/pubmed/37965035
http://dx.doi.org/10.3389/fpls.2023.1268726
_version_ 1785146824143667200
author Nagar, Arvind
Gowthami, Ravi
Sureja, Amish Kumar
Munshi, Anilabha Das
Verma, Manjusha
Singh, Awani Kumar
Mallick, Niharika
Singh, Jogendra
Chander, Subhash
Shankar, Muthusamy
Pathania, Pooja
Rajkumar, Subramani
author_facet Nagar, Arvind
Gowthami, Ravi
Sureja, Amish Kumar
Munshi, Anilabha Das
Verma, Manjusha
Singh, Awani Kumar
Mallick, Niharika
Singh, Jogendra
Chander, Subhash
Shankar, Muthusamy
Pathania, Pooja
Rajkumar, Subramani
author_sort Nagar, Arvind
collection PubMed
description This study aimed to develop a long-term pollen storage protocol for Luffa species (L. acutangula, L. cylindrica, L. echinata, and L. graveolens) and assess its potential for crop improvement. The optimal medium for in vitro pollen germination varied by species, with Brewbaker and Kwack (BK) medium with 10% sucrose suitable for L. acutangula, L. cylindrica, and L. echinata, and BK medium with 3% sucrose ideal for L. graveolens. Overestimation in staining tests compared to in vitro pollen germination was observed. The best results for cryopreservation were achieved with desiccation periods of 20, 30, and 40 min, maintaining moisture content between 14.04% and 18.55%. Pollen viability was negatively correlated with storage temperature (25, 4, and −20°C) and duration. Cryopreserved pollen at −196°C exhibited the highest viability over a prolonged period (2 months) and was comparable to fresh pollen in terms of germination, ovule fertilization, and fruit and seed set. This study presents a simple and reproducible pollen cryopreservation protocol applicable across Luffa species, facilitating long-term storage and its use in crop improvement efforts.
format Online
Article
Text
id pubmed-10641756
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-106417562023-11-14 Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency Nagar, Arvind Gowthami, Ravi Sureja, Amish Kumar Munshi, Anilabha Das Verma, Manjusha Singh, Awani Kumar Mallick, Niharika Singh, Jogendra Chander, Subhash Shankar, Muthusamy Pathania, Pooja Rajkumar, Subramani Front Plant Sci Plant Science This study aimed to develop a long-term pollen storage protocol for Luffa species (L. acutangula, L. cylindrica, L. echinata, and L. graveolens) and assess its potential for crop improvement. The optimal medium for in vitro pollen germination varied by species, with Brewbaker and Kwack (BK) medium with 10% sucrose suitable for L. acutangula, L. cylindrica, and L. echinata, and BK medium with 3% sucrose ideal for L. graveolens. Overestimation in staining tests compared to in vitro pollen germination was observed. The best results for cryopreservation were achieved with desiccation periods of 20, 30, and 40 min, maintaining moisture content between 14.04% and 18.55%. Pollen viability was negatively correlated with storage temperature (25, 4, and −20°C) and duration. Cryopreserved pollen at −196°C exhibited the highest viability over a prolonged period (2 months) and was comparable to fresh pollen in terms of germination, ovule fertilization, and fruit and seed set. This study presents a simple and reproducible pollen cryopreservation protocol applicable across Luffa species, facilitating long-term storage and its use in crop improvement efforts. Frontiers Media S.A. 2023-10-27 /pmc/articles/PMC10641756/ /pubmed/37965035 http://dx.doi.org/10.3389/fpls.2023.1268726 Text en Copyright © 2023 Nagar, Gowthami, Sureja, Munshi, Verma, Singh, Mallick, Singh, Chander, Shankar, Pathania and Rajkumar https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Nagar, Arvind
Gowthami, Ravi
Sureja, Amish Kumar
Munshi, Anilabha Das
Verma, Manjusha
Singh, Awani Kumar
Mallick, Niharika
Singh, Jogendra
Chander, Subhash
Shankar, Muthusamy
Pathania, Pooja
Rajkumar, Subramani
Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency
title Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency
title_full Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency
title_fullStr Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency
title_full_unstemmed Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency
title_short Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency
title_sort simple cryopreservation protocol for luffa pollen: enhancing breeding efficiency
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641756/
https://www.ncbi.nlm.nih.gov/pubmed/37965035
http://dx.doi.org/10.3389/fpls.2023.1268726
work_keys_str_mv AT nagararvind simplecryopreservationprotocolforluffapollenenhancingbreedingefficiency
AT gowthamiravi simplecryopreservationprotocolforluffapollenenhancingbreedingefficiency
AT surejaamishkumar simplecryopreservationprotocolforluffapollenenhancingbreedingefficiency
AT munshianilabhadas simplecryopreservationprotocolforluffapollenenhancingbreedingefficiency
AT vermamanjusha simplecryopreservationprotocolforluffapollenenhancingbreedingefficiency
AT singhawanikumar simplecryopreservationprotocolforluffapollenenhancingbreedingefficiency
AT mallickniharika simplecryopreservationprotocolforluffapollenenhancingbreedingefficiency
AT singhjogendra simplecryopreservationprotocolforluffapollenenhancingbreedingefficiency
AT chandersubhash simplecryopreservationprotocolforluffapollenenhancingbreedingefficiency
AT shankarmuthusamy simplecryopreservationprotocolforluffapollenenhancingbreedingefficiency
AT pathaniapooja simplecryopreservationprotocolforluffapollenenhancingbreedingefficiency
AT rajkumarsubramani simplecryopreservationprotocolforluffapollenenhancingbreedingefficiency