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Composition, function, and timing: exploring the early-life gut microbiota in piglets for probiotic interventions

BACKGROUND: The establishment of a robust gut microbiota in piglets during their early developmental stage holds the potential for long-term advantageous effects. However, the optimal timeframe for introducing probiotics to achieve this outcome remains uncertain. RESULTS: In the context of this inve...

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Autores principales: Quan, Jianping, Xu, Cineng, Ruan, Donglin, Ye, Yong, Qiu, Yibin, Wu, Jie, Zhou, Shenping, Luan, Menghao, Zhao, Xiang, Chen, Yue, Lin, Danyang, Sun, Ying, Yang, Jifei, Zheng, Enqin, Cai, Gengyuan, Wu, Zhenfang, Yang, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641937/
https://www.ncbi.nlm.nih.gov/pubmed/37957747
http://dx.doi.org/10.1186/s40104-023-00943-z
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author Quan, Jianping
Xu, Cineng
Ruan, Donglin
Ye, Yong
Qiu, Yibin
Wu, Jie
Zhou, Shenping
Luan, Menghao
Zhao, Xiang
Chen, Yue
Lin, Danyang
Sun, Ying
Yang, Jifei
Zheng, Enqin
Cai, Gengyuan
Wu, Zhenfang
Yang, Jie
author_facet Quan, Jianping
Xu, Cineng
Ruan, Donglin
Ye, Yong
Qiu, Yibin
Wu, Jie
Zhou, Shenping
Luan, Menghao
Zhao, Xiang
Chen, Yue
Lin, Danyang
Sun, Ying
Yang, Jifei
Zheng, Enqin
Cai, Gengyuan
Wu, Zhenfang
Yang, Jie
author_sort Quan, Jianping
collection PubMed
description BACKGROUND: The establishment of a robust gut microbiota in piglets during their early developmental stage holds the potential for long-term advantageous effects. However, the optimal timeframe for introducing probiotics to achieve this outcome remains uncertain. RESULTS: In the context of this investigation, we conducted a longitudinal assessment of the fecal microbiota of 63 piglets at three distinct pre-weaning time points. Simultaneously, we gathered vaginal and fecal samples from 23 sows. Employing 16S rRNA gene and metagenomic sequencing methodologies, we conducted a comprehensive analysis of the fluctuation patterns in microbial composition, functional capacity, interaction networks, and colonization resistance within the gut microbiota of piglets. As the piglets progressed in age, discernible modifications in intestinal microbial diversity, composition, and function were observed. A source-tracking analysis unveiled the pivotal role of fecal and vaginal microbiota derived from sows in populating the gut microbiota of neonatal piglets. By D21, the microbial interaction network displayed a more concise and efficient configuration, accompanied by enhanced colonization resistance relative to the other two time points. Moreover, we identified three strains of Ruminococcus sp. at D10 as potential candidates for improving piglets' weight gain during the weaning phase. CONCLUSIONS: The findings of this study propose that D10 represents the most opportune juncture for the introduction of external probiotic interventions during the early stages of piglet development. This investigation augments our comprehension of the microbiota dynamics in early-life of piglets and offers valuable insights for guiding forthcoming probiotic interventions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40104-023-00943-z.
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spelling pubmed-106419372023-11-14 Composition, function, and timing: exploring the early-life gut microbiota in piglets for probiotic interventions Quan, Jianping Xu, Cineng Ruan, Donglin Ye, Yong Qiu, Yibin Wu, Jie Zhou, Shenping Luan, Menghao Zhao, Xiang Chen, Yue Lin, Danyang Sun, Ying Yang, Jifei Zheng, Enqin Cai, Gengyuan Wu, Zhenfang Yang, Jie J Anim Sci Biotechnol Research BACKGROUND: The establishment of a robust gut microbiota in piglets during their early developmental stage holds the potential for long-term advantageous effects. However, the optimal timeframe for introducing probiotics to achieve this outcome remains uncertain. RESULTS: In the context of this investigation, we conducted a longitudinal assessment of the fecal microbiota of 63 piglets at three distinct pre-weaning time points. Simultaneously, we gathered vaginal and fecal samples from 23 sows. Employing 16S rRNA gene and metagenomic sequencing methodologies, we conducted a comprehensive analysis of the fluctuation patterns in microbial composition, functional capacity, interaction networks, and colonization resistance within the gut microbiota of piglets. As the piglets progressed in age, discernible modifications in intestinal microbial diversity, composition, and function were observed. A source-tracking analysis unveiled the pivotal role of fecal and vaginal microbiota derived from sows in populating the gut microbiota of neonatal piglets. By D21, the microbial interaction network displayed a more concise and efficient configuration, accompanied by enhanced colonization resistance relative to the other two time points. Moreover, we identified three strains of Ruminococcus sp. at D10 as potential candidates for improving piglets' weight gain during the weaning phase. CONCLUSIONS: The findings of this study propose that D10 represents the most opportune juncture for the introduction of external probiotic interventions during the early stages of piglet development. This investigation augments our comprehension of the microbiota dynamics in early-life of piglets and offers valuable insights for guiding forthcoming probiotic interventions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40104-023-00943-z. BioMed Central 2023-11-13 /pmc/articles/PMC10641937/ /pubmed/37957747 http://dx.doi.org/10.1186/s40104-023-00943-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Quan, Jianping
Xu, Cineng
Ruan, Donglin
Ye, Yong
Qiu, Yibin
Wu, Jie
Zhou, Shenping
Luan, Menghao
Zhao, Xiang
Chen, Yue
Lin, Danyang
Sun, Ying
Yang, Jifei
Zheng, Enqin
Cai, Gengyuan
Wu, Zhenfang
Yang, Jie
Composition, function, and timing: exploring the early-life gut microbiota in piglets for probiotic interventions
title Composition, function, and timing: exploring the early-life gut microbiota in piglets for probiotic interventions
title_full Composition, function, and timing: exploring the early-life gut microbiota in piglets for probiotic interventions
title_fullStr Composition, function, and timing: exploring the early-life gut microbiota in piglets for probiotic interventions
title_full_unstemmed Composition, function, and timing: exploring the early-life gut microbiota in piglets for probiotic interventions
title_short Composition, function, and timing: exploring the early-life gut microbiota in piglets for probiotic interventions
title_sort composition, function, and timing: exploring the early-life gut microbiota in piglets for probiotic interventions
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10641937/
https://www.ncbi.nlm.nih.gov/pubmed/37957747
http://dx.doi.org/10.1186/s40104-023-00943-z
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