Evaluation of next generation sequencing approaches for SARS-CoV-2

Within public health control strategies for SARS-CoV-2, whole genome sequencing (WGS) is essential for tracking viral spread and monitoring the emergence of variants which may impair the effectiveness of vaccines, diagnostic methods, and therapeutics. In this manuscript different strategies for SARS...

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Detalles Bibliográficos
Autores principales: Curini, Valentina, Ancora, Massimo, Jurisic, Lucija, Di Lollo, Valeria, Secondini, Barbara, Mincarelli, Luana Fiorella, Caporale, Marialuigia, Puglia, Ilaria, Di Gialleonardo, Luigina, Mangone, Iolanda, Di Domenico, Marco, Di Pasquale, Adriano, Lorusso, Alessio, Marcacci, Maurilia, Cammà, Cesare
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643093/
https://www.ncbi.nlm.nih.gov/pubmed/38027571
http://dx.doi.org/10.1016/j.heliyon.2023.e21101
Descripción
Sumario:Within public health control strategies for SARS-CoV-2, whole genome sequencing (WGS) is essential for tracking viral spread and monitoring the emergence of variants which may impair the effectiveness of vaccines, diagnostic methods, and therapeutics. In this manuscript different strategies for SARS-CoV-2 WGS including metagenomic shotgun (SG), library enrichment by myBaits® Expert Virus-SARS-CoV-2 (Arbor Biosciences), nCoV-2019 sequencing protocol, ampliseq approach by Swift Amplicon® SARS-CoV-2 Panel kit (Swift Biosciences), and Illumina COVIDSeq Test (Illumina Inc.), were evaluated in order to identify the best approach in terms of results, labour, and costs. The analysis revealed that Illumina COVIDSeq Test (Illumina Inc.) is the best choice for a cost-effective, time-consuming production of consensus sequences.

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