Protocol for detecting threonine deaminase activity in fission yeast cell lysates

Threonine deaminase catalyzes the first step of isoleucine biosynthesis from threonine. In this protocol, we describe the process of measuring the enzymatic activity of threonine deaminase in the fission yeast cell lysate, which is catalyzed by Tda1. First, we describe the process of preparing cell...

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Detalles Bibliográficos
Autores principales: Sasaki, Mayuki, Nishimura, Shinichi, Matsuyama, Akihisa, Yoshida, Minoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643616/
https://www.ncbi.nlm.nih.gov/pubmed/37910512
http://dx.doi.org/10.1016/j.xpro.2023.102675
Descripción
Sumario:Threonine deaminase catalyzes the first step of isoleucine biosynthesis from threonine. In this protocol, we describe the process of measuring the enzymatic activity of threonine deaminase in the fission yeast cell lysate, which is catalyzed by Tda1. First, we describe the process of preparing cell lysates from fission yeast cell cultures. Subsequently, we explain how to measure the threonine deaminase activity using threonine or serine as a substrate. For complete details on the use and execution of this protocol, please refer to Sasaki et al. (2022).(1)

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