Protocol for detecting threonine deaminase activity in fission yeast cell lysates

Threonine deaminase catalyzes the first step of isoleucine biosynthesis from threonine. In this protocol, we describe the process of measuring the enzymatic activity of threonine deaminase in the fission yeast cell lysate, which is catalyzed by Tda1. First, we describe the process of preparing cell...

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Detalles Bibliográficos
Autores principales: Sasaki, Mayuki, Nishimura, Shinichi, Matsuyama, Akihisa, Yoshida, Minoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643616/
https://www.ncbi.nlm.nih.gov/pubmed/37910512
http://dx.doi.org/10.1016/j.xpro.2023.102675
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author Sasaki, Mayuki
Nishimura, Shinichi
Matsuyama, Akihisa
Yoshida, Minoru
author_facet Sasaki, Mayuki
Nishimura, Shinichi
Matsuyama, Akihisa
Yoshida, Minoru
author_sort Sasaki, Mayuki
collection PubMed
description Threonine deaminase catalyzes the first step of isoleucine biosynthesis from threonine. In this protocol, we describe the process of measuring the enzymatic activity of threonine deaminase in the fission yeast cell lysate, which is catalyzed by Tda1. First, we describe the process of preparing cell lysates from fission yeast cell cultures. Subsequently, we explain how to measure the threonine deaminase activity using threonine or serine as a substrate. For complete details on the use and execution of this protocol, please refer to Sasaki et al. (2022).(1)
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spelling pubmed-106436162023-10-31 Protocol for detecting threonine deaminase activity in fission yeast cell lysates Sasaki, Mayuki Nishimura, Shinichi Matsuyama, Akihisa Yoshida, Minoru STAR Protoc Protocol Threonine deaminase catalyzes the first step of isoleucine biosynthesis from threonine. In this protocol, we describe the process of measuring the enzymatic activity of threonine deaminase in the fission yeast cell lysate, which is catalyzed by Tda1. First, we describe the process of preparing cell lysates from fission yeast cell cultures. Subsequently, we explain how to measure the threonine deaminase activity using threonine or serine as a substrate. For complete details on the use and execution of this protocol, please refer to Sasaki et al. (2022).(1) Elsevier 2023-10-31 /pmc/articles/PMC10643616/ /pubmed/37910512 http://dx.doi.org/10.1016/j.xpro.2023.102675 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Sasaki, Mayuki
Nishimura, Shinichi
Matsuyama, Akihisa
Yoshida, Minoru
Protocol for detecting threonine deaminase activity in fission yeast cell lysates
title Protocol for detecting threonine deaminase activity in fission yeast cell lysates
title_full Protocol for detecting threonine deaminase activity in fission yeast cell lysates
title_fullStr Protocol for detecting threonine deaminase activity in fission yeast cell lysates
title_full_unstemmed Protocol for detecting threonine deaminase activity in fission yeast cell lysates
title_short Protocol for detecting threonine deaminase activity in fission yeast cell lysates
title_sort protocol for detecting threonine deaminase activity in fission yeast cell lysates
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643616/
https://www.ncbi.nlm.nih.gov/pubmed/37910512
http://dx.doi.org/10.1016/j.xpro.2023.102675
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