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Integration of high-resolution imaging through scattering medium into a disposable micro-endoscope via projection of 2D spots-array

The objective of this research includes integration of high-resolution imaging through scattering medium, such as blood, into a disposable micro-endoscope. A fiber laser integrated into the micro-endoscope as part of its illumination channel, allows to project a tunable array of spots of light onto...

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Autores principales: Elkabetz, Shimon, Herman, Oran, Meiri, Amihai, Shahmoon, Asaf, Zalevsky, Zeev
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643705/
https://www.ncbi.nlm.nih.gov/pubmed/37957202
http://dx.doi.org/10.1038/s41598-023-46657-0
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author Elkabetz, Shimon
Herman, Oran
Meiri, Amihai
Shahmoon, Asaf
Zalevsky, Zeev
author_facet Elkabetz, Shimon
Herman, Oran
Meiri, Amihai
Shahmoon, Asaf
Zalevsky, Zeev
author_sort Elkabetz, Shimon
collection PubMed
description The objective of this research includes integration of high-resolution imaging through scattering medium, such as blood, into a disposable micro-endoscope. A fiber laser integrated into the micro-endoscope as part of its illumination channel, allows to project a tunable array of spots of light onto an object, that is located behind the scattering medium. We have a laser fiber as part of the illumination channel of a disposable micro-endoscope. By using proper optics, we convert the temporal modulation of the laser into spatial distribution. Thus, the result is generation of spatial spots when using a pulsed laser. The detection channel is a holographic recording of the collected back scattered light, that allows extraction of the electrical field. By time integrating the field we obtain the realization of the spatial array of illumination spots formed on top of the inspected object and behind the scattering medium. By changing the temporal modulation of the illumination laser (changing its temporal photonic signals), we can tune the positions of the spots in the illumination array. If the distance between the projected spots is larger than the imaging resolution, then by applying localization microscopy algorithms together with scanning of the position of the spots in the array, will yield a high-resolution reconstruction of the inspected object. We theoretically and experimentally demonstrate the discussed operation principle and show the potential of the proposed concept as a modality in medical endoscopic procedures.
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spelling pubmed-106437052023-11-13 Integration of high-resolution imaging through scattering medium into a disposable micro-endoscope via projection of 2D spots-array Elkabetz, Shimon Herman, Oran Meiri, Amihai Shahmoon, Asaf Zalevsky, Zeev Sci Rep Article The objective of this research includes integration of high-resolution imaging through scattering medium, such as blood, into a disposable micro-endoscope. A fiber laser integrated into the micro-endoscope as part of its illumination channel, allows to project a tunable array of spots of light onto an object, that is located behind the scattering medium. We have a laser fiber as part of the illumination channel of a disposable micro-endoscope. By using proper optics, we convert the temporal modulation of the laser into spatial distribution. Thus, the result is generation of spatial spots when using a pulsed laser. The detection channel is a holographic recording of the collected back scattered light, that allows extraction of the electrical field. By time integrating the field we obtain the realization of the spatial array of illumination spots formed on top of the inspected object and behind the scattering medium. By changing the temporal modulation of the illumination laser (changing its temporal photonic signals), we can tune the positions of the spots in the illumination array. If the distance between the projected spots is larger than the imaging resolution, then by applying localization microscopy algorithms together with scanning of the position of the spots in the array, will yield a high-resolution reconstruction of the inspected object. We theoretically and experimentally demonstrate the discussed operation principle and show the potential of the proposed concept as a modality in medical endoscopic procedures. Nature Publishing Group UK 2023-11-13 /pmc/articles/PMC10643705/ /pubmed/37957202 http://dx.doi.org/10.1038/s41598-023-46657-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Elkabetz, Shimon
Herman, Oran
Meiri, Amihai
Shahmoon, Asaf
Zalevsky, Zeev
Integration of high-resolution imaging through scattering medium into a disposable micro-endoscope via projection of 2D spots-array
title Integration of high-resolution imaging through scattering medium into a disposable micro-endoscope via projection of 2D spots-array
title_full Integration of high-resolution imaging through scattering medium into a disposable micro-endoscope via projection of 2D spots-array
title_fullStr Integration of high-resolution imaging through scattering medium into a disposable micro-endoscope via projection of 2D spots-array
title_full_unstemmed Integration of high-resolution imaging through scattering medium into a disposable micro-endoscope via projection of 2D spots-array
title_short Integration of high-resolution imaging through scattering medium into a disposable micro-endoscope via projection of 2D spots-array
title_sort integration of high-resolution imaging through scattering medium into a disposable micro-endoscope via projection of 2d spots-array
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643705/
https://www.ncbi.nlm.nih.gov/pubmed/37957202
http://dx.doi.org/10.1038/s41598-023-46657-0
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