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Differential gene expression during floral transition in pineapple

Pineapple ( Ananas comosus var. comosus) and ornamental bromeliads are commercially induced to flower by treatment with ethylene or its analogs. The apex is transformed from a vegetative to a floral meristem and shows morphological changes in 8 to 10 days, with flowers developing 8 to 10 weeks later...

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Autores principales: Paull, Robert E., Ksouri, Najla, Kantar, Michael, Zerpa‐Catanho, Dessireé, Chen, Nancy Jung, Uruu, Gail, Yue, Jingjing, Guo, Shiyong, Zheng, Yun, Wai, Ching Man Jennifer, Ming, Ray
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10644199/
https://www.ncbi.nlm.nih.gov/pubmed/38028646
http://dx.doi.org/10.1002/pld3.541
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author Paull, Robert E.
Ksouri, Najla
Kantar, Michael
Zerpa‐Catanho, Dessireé
Chen, Nancy Jung
Uruu, Gail
Yue, Jingjing
Guo, Shiyong
Zheng, Yun
Wai, Ching Man Jennifer
Ming, Ray
author_facet Paull, Robert E.
Ksouri, Najla
Kantar, Michael
Zerpa‐Catanho, Dessireé
Chen, Nancy Jung
Uruu, Gail
Yue, Jingjing
Guo, Shiyong
Zheng, Yun
Wai, Ching Man Jennifer
Ming, Ray
author_sort Paull, Robert E.
collection PubMed
description Pineapple ( Ananas comosus var. comosus) and ornamental bromeliads are commercially induced to flower by treatment with ethylene or its analogs. The apex is transformed from a vegetative to a floral meristem and shows morphological changes in 8 to 10 days, with flowers developing 8 to 10 weeks later. During eight sampling stages ranging from 6 h to 8 days after treatment, 7961 genes were found to exhibit differential expression (DE) after the application of ethylene. In the first 3 days after treatment, there was little change in ethylene synthesis or in the early stages of the ethylene response. Subsequently, three ethylene response transcription factors (ERTF) were up‐regulated and the potential gene targets were predicted to be the positive flowering regulator CONSTANS‐like 3 (CO), a WUSCHEL gene, two APETALA1/FRUITFULL (AP1/FUL) genes, an epidermal patterning gene, and a jasmonic acid synthesis gene. We confirm that pineapple has lost the flowering repressor FLOWERING LOCUS C. At the initial stages, the SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) was not significantly involved in this transition. Another WUSCHEL gene and a PHD homeobox transcription factor, though not apparent direct targets of ERTF, were up‐regulated within a day of treatment, their predicted targets being the up‐regulated CO, auxin response factors, SQUAMOSA, and histone H3 genes with suppression of abscisic acid response genes. The FLOWERING LOCUS T (FT), TERMINAL FLOWER (TFL), AGAMOUS‐like APETELAR (AP2), and SEPETALA (SEP) increased rapidly within 2 to 3 days after ethylene treatment. Two FT genes were up‐regulated at the apex and not at the leaf bases after treatment, suggesting that transport did not occur. These results indicated that the ethylene response in pineapple and possibly most bromeliads act directly to promote the vegetative to flower transition via APETALA1/FRUITFULL (AP1/FUL) and its interaction with SPL, FT, TFL, SEP, and AP2. A model based on AP2/ERTF DE and predicted DE target genes was developed to give focus to future research. The identified candidate genes are potential targets for genetic manipulation to determine their molecular role in flower transition.
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spelling pubmed-106441992023-11-14 Differential gene expression during floral transition in pineapple Paull, Robert E. Ksouri, Najla Kantar, Michael Zerpa‐Catanho, Dessireé Chen, Nancy Jung Uruu, Gail Yue, Jingjing Guo, Shiyong Zheng, Yun Wai, Ching Man Jennifer Ming, Ray Plant Direct Research Articles Pineapple ( Ananas comosus var. comosus) and ornamental bromeliads are commercially induced to flower by treatment with ethylene or its analogs. The apex is transformed from a vegetative to a floral meristem and shows morphological changes in 8 to 10 days, with flowers developing 8 to 10 weeks later. During eight sampling stages ranging from 6 h to 8 days after treatment, 7961 genes were found to exhibit differential expression (DE) after the application of ethylene. In the first 3 days after treatment, there was little change in ethylene synthesis or in the early stages of the ethylene response. Subsequently, three ethylene response transcription factors (ERTF) were up‐regulated and the potential gene targets were predicted to be the positive flowering regulator CONSTANS‐like 3 (CO), a WUSCHEL gene, two APETALA1/FRUITFULL (AP1/FUL) genes, an epidermal patterning gene, and a jasmonic acid synthesis gene. We confirm that pineapple has lost the flowering repressor FLOWERING LOCUS C. At the initial stages, the SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) was not significantly involved in this transition. Another WUSCHEL gene and a PHD homeobox transcription factor, though not apparent direct targets of ERTF, were up‐regulated within a day of treatment, their predicted targets being the up‐regulated CO, auxin response factors, SQUAMOSA, and histone H3 genes with suppression of abscisic acid response genes. The FLOWERING LOCUS T (FT), TERMINAL FLOWER (TFL), AGAMOUS‐like APETELAR (AP2), and SEPETALA (SEP) increased rapidly within 2 to 3 days after ethylene treatment. Two FT genes were up‐regulated at the apex and not at the leaf bases after treatment, suggesting that transport did not occur. These results indicated that the ethylene response in pineapple and possibly most bromeliads act directly to promote the vegetative to flower transition via APETALA1/FRUITFULL (AP1/FUL) and its interaction with SPL, FT, TFL, SEP, and AP2. A model based on AP2/ERTF DE and predicted DE target genes was developed to give focus to future research. The identified candidate genes are potential targets for genetic manipulation to determine their molecular role in flower transition. John Wiley and Sons Inc. 2023-11-14 /pmc/articles/PMC10644199/ /pubmed/38028646 http://dx.doi.org/10.1002/pld3.541 Text en © 2023 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Paull, Robert E.
Ksouri, Najla
Kantar, Michael
Zerpa‐Catanho, Dessireé
Chen, Nancy Jung
Uruu, Gail
Yue, Jingjing
Guo, Shiyong
Zheng, Yun
Wai, Ching Man Jennifer
Ming, Ray
Differential gene expression during floral transition in pineapple
title Differential gene expression during floral transition in pineapple
title_full Differential gene expression during floral transition in pineapple
title_fullStr Differential gene expression during floral transition in pineapple
title_full_unstemmed Differential gene expression during floral transition in pineapple
title_short Differential gene expression during floral transition in pineapple
title_sort differential gene expression during floral transition in pineapple
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10644199/
https://www.ncbi.nlm.nih.gov/pubmed/38028646
http://dx.doi.org/10.1002/pld3.541
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