Cargando…

Isolation and identification of Tete virus group (Peribunyaviridae: Orthobunyavirus) from Culicoides biting midges collected in Lichuan County, China

In July 2018, a virus (JXLC1806-2) was isolated from Culicoides biting midges collected in Lichuan County, Jiangxi Province, China. The virus isolate showed significant cytopathic effects within 48 hours after inoculation with mammalian cells (BHK-21). JXLC1806-2 virus could form plaques in BHK-21 c...

Descripción completa

Detalles Bibliográficos
Autores principales: Yin, Qikai, Cheng, Rui, Xu, Xiuyan, Xu, Ziqian, Wang, Jing, Fu, Shihong, Xu, Hongbin, Zhang, Shaozai, He, Ying, Li, Fan, Xu, Songtao, Lu, Xiaoqing, Wang, Huanyu, Wang, Bin, Liang, Guodong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10644344/
https://www.ncbi.nlm.nih.gov/pubmed/38029255
http://dx.doi.org/10.3389/fcimb.2023.1193184
_version_ 1785134527487672320
author Yin, Qikai
Cheng, Rui
Xu, Xiuyan
Xu, Ziqian
Wang, Jing
Fu, Shihong
Xu, Hongbin
Zhang, Shaozai
He, Ying
Li, Fan
Xu, Songtao
Lu, Xiaoqing
Wang, Huanyu
Wang, Bin
Liang, Guodong
author_facet Yin, Qikai
Cheng, Rui
Xu, Xiuyan
Xu, Ziqian
Wang, Jing
Fu, Shihong
Xu, Hongbin
Zhang, Shaozai
He, Ying
Li, Fan
Xu, Songtao
Lu, Xiaoqing
Wang, Huanyu
Wang, Bin
Liang, Guodong
author_sort Yin, Qikai
collection PubMed
description In July 2018, a virus (JXLC1806-2) was isolated from Culicoides biting midges collected in Lichuan County, Jiangxi Province, China. The virus isolate showed significant cytopathic effects within 48 hours after inoculation with mammalian cells (BHK-21). JXLC1806-2 virus could form plaques in BHK-21 cells, and the virus titer was 1×10(5.6) pfu/mL. After inoculation with the virus, suckling mice developed disease and died. The nucleotide and amino sequence analysis showed that the JXLC1806-2 virus genome was composed of S, M and L segments. Phylogenetic analysis showed that the S, M and L genes of JXLC1806-2 virus belonged to the Tete serogroup, Orthobunyavirus, but formed an independent evolutionary branch from the other members of the Tete serogroup. The results showed that the JXLC1806-2 virus, which was named as Lichuan virus, is a new member of Tete serogroup, and this is the first time that a Tete serogroup virus has been isolated in China.
format Online
Article
Text
id pubmed-10644344
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-106443442023-01-01 Isolation and identification of Tete virus group (Peribunyaviridae: Orthobunyavirus) from Culicoides biting midges collected in Lichuan County, China Yin, Qikai Cheng, Rui Xu, Xiuyan Xu, Ziqian Wang, Jing Fu, Shihong Xu, Hongbin Zhang, Shaozai He, Ying Li, Fan Xu, Songtao Lu, Xiaoqing Wang, Huanyu Wang, Bin Liang, Guodong Front Cell Infect Microbiol Cellular and Infection Microbiology In July 2018, a virus (JXLC1806-2) was isolated from Culicoides biting midges collected in Lichuan County, Jiangxi Province, China. The virus isolate showed significant cytopathic effects within 48 hours after inoculation with mammalian cells (BHK-21). JXLC1806-2 virus could form plaques in BHK-21 cells, and the virus titer was 1×10(5.6) pfu/mL. After inoculation with the virus, suckling mice developed disease and died. The nucleotide and amino sequence analysis showed that the JXLC1806-2 virus genome was composed of S, M and L segments. Phylogenetic analysis showed that the S, M and L genes of JXLC1806-2 virus belonged to the Tete serogroup, Orthobunyavirus, but formed an independent evolutionary branch from the other members of the Tete serogroup. The results showed that the JXLC1806-2 virus, which was named as Lichuan virus, is a new member of Tete serogroup, and this is the first time that a Tete serogroup virus has been isolated in China. Frontiers Media S.A. 2023-10-31 /pmc/articles/PMC10644344/ /pubmed/38029255 http://dx.doi.org/10.3389/fcimb.2023.1193184 Text en Copyright © 2023 Yin, Cheng, Xu, Xu, Wang, Fu, Xu, Zhang, He, Li, Xu, Lu, Wang, Wang and Liang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Yin, Qikai
Cheng, Rui
Xu, Xiuyan
Xu, Ziqian
Wang, Jing
Fu, Shihong
Xu, Hongbin
Zhang, Shaozai
He, Ying
Li, Fan
Xu, Songtao
Lu, Xiaoqing
Wang, Huanyu
Wang, Bin
Liang, Guodong
Isolation and identification of Tete virus group (Peribunyaviridae: Orthobunyavirus) from Culicoides biting midges collected in Lichuan County, China
title Isolation and identification of Tete virus group (Peribunyaviridae: Orthobunyavirus) from Culicoides biting midges collected in Lichuan County, China
title_full Isolation and identification of Tete virus group (Peribunyaviridae: Orthobunyavirus) from Culicoides biting midges collected in Lichuan County, China
title_fullStr Isolation and identification of Tete virus group (Peribunyaviridae: Orthobunyavirus) from Culicoides biting midges collected in Lichuan County, China
title_full_unstemmed Isolation and identification of Tete virus group (Peribunyaviridae: Orthobunyavirus) from Culicoides biting midges collected in Lichuan County, China
title_short Isolation and identification of Tete virus group (Peribunyaviridae: Orthobunyavirus) from Culicoides biting midges collected in Lichuan County, China
title_sort isolation and identification of tete virus group (peribunyaviridae: orthobunyavirus) from culicoides biting midges collected in lichuan county, china
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10644344/
https://www.ncbi.nlm.nih.gov/pubmed/38029255
http://dx.doi.org/10.3389/fcimb.2023.1193184
work_keys_str_mv AT yinqikai isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT chengrui isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT xuxiuyan isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT xuziqian isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT wangjing isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT fushihong isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT xuhongbin isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT zhangshaozai isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT heying isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT lifan isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT xusongtao isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT luxiaoqing isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT wanghuanyu isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT wangbin isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina
AT liangguodong isolationandidentificationoftetevirusgroupperibunyaviridaeorthobunyavirusfromculicoidesbitingmidgescollectedinlichuancountychina