Aging and the impact of global DNA methylation, telomere shortening, and total oxidative status on sarcopenia and frailty syndrome

Aging is a biological event that influences many organs and systems. Both sarcopenia and frailty syndrome refer to geriatric conditions with overlapping phenotypes. Many mechanisms are involved in the aging process such as DNA methylation telomeres which are susceptible to oxidative stress, and infl...

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Autores principales: Kmiołek, Tomasz, Filipowicz, Gabriela, Bogucka, Diana, Wajda, Anna, Ejma-Multański, Adam, Stypińska, Barbara, Modzelewska, Ewa, Kaliberda, Yana, Radkowski, Marcin, Targowski, Tomasz, Wrona, Julia, Paradowska-Gorycka, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10644469/
https://www.ncbi.nlm.nih.gov/pubmed/37964387
http://dx.doi.org/10.1186/s12979-023-00384-2
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author Kmiołek, Tomasz
Filipowicz, Gabriela
Bogucka, Diana
Wajda, Anna
Ejma-Multański, Adam
Stypińska, Barbara
Modzelewska, Ewa
Kaliberda, Yana
Radkowski, Marcin
Targowski, Tomasz
Wrona, Julia
Paradowska-Gorycka, Agnieszka
author_facet Kmiołek, Tomasz
Filipowicz, Gabriela
Bogucka, Diana
Wajda, Anna
Ejma-Multański, Adam
Stypińska, Barbara
Modzelewska, Ewa
Kaliberda, Yana
Radkowski, Marcin
Targowski, Tomasz
Wrona, Julia
Paradowska-Gorycka, Agnieszka
author_sort Kmiołek, Tomasz
collection PubMed
description Aging is a biological event that influences many organs and systems. Both sarcopenia and frailty syndrome refer to geriatric conditions with overlapping phenotypes. Many mechanisms are involved in the aging process such as DNA methylation telomeres which are susceptible to oxidative stress, and inflammations which result in telomere shortening, leading to chromosomal instability. The study aimed to determine the associations between these processes, frailty and sarcopenia syndrome. Global DNA methylation was analyzed using the ELISA method. Telomere length was analyzed using qPCR. Total oxidative status (TOS) was analyzed using a colorimetric method. The present study revealed that the main factor affecting methylation, telomeres length and level of total oxidant stress was age.
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spelling pubmed-106444692023-11-14 Aging and the impact of global DNA methylation, telomere shortening, and total oxidative status on sarcopenia and frailty syndrome Kmiołek, Tomasz Filipowicz, Gabriela Bogucka, Diana Wajda, Anna Ejma-Multański, Adam Stypińska, Barbara Modzelewska, Ewa Kaliberda, Yana Radkowski, Marcin Targowski, Tomasz Wrona, Julia Paradowska-Gorycka, Agnieszka Immun Ageing Research Aging is a biological event that influences many organs and systems. Both sarcopenia and frailty syndrome refer to geriatric conditions with overlapping phenotypes. Many mechanisms are involved in the aging process such as DNA methylation telomeres which are susceptible to oxidative stress, and inflammations which result in telomere shortening, leading to chromosomal instability. The study aimed to determine the associations between these processes, frailty and sarcopenia syndrome. Global DNA methylation was analyzed using the ELISA method. Telomere length was analyzed using qPCR. Total oxidative status (TOS) was analyzed using a colorimetric method. The present study revealed that the main factor affecting methylation, telomeres length and level of total oxidant stress was age. BioMed Central 2023-11-14 /pmc/articles/PMC10644469/ /pubmed/37964387 http://dx.doi.org/10.1186/s12979-023-00384-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kmiołek, Tomasz
Filipowicz, Gabriela
Bogucka, Diana
Wajda, Anna
Ejma-Multański, Adam
Stypińska, Barbara
Modzelewska, Ewa
Kaliberda, Yana
Radkowski, Marcin
Targowski, Tomasz
Wrona, Julia
Paradowska-Gorycka, Agnieszka
Aging and the impact of global DNA methylation, telomere shortening, and total oxidative status on sarcopenia and frailty syndrome
title Aging and the impact of global DNA methylation, telomere shortening, and total oxidative status on sarcopenia and frailty syndrome
title_full Aging and the impact of global DNA methylation, telomere shortening, and total oxidative status on sarcopenia and frailty syndrome
title_fullStr Aging and the impact of global DNA methylation, telomere shortening, and total oxidative status on sarcopenia and frailty syndrome
title_full_unstemmed Aging and the impact of global DNA methylation, telomere shortening, and total oxidative status on sarcopenia and frailty syndrome
title_short Aging and the impact of global DNA methylation, telomere shortening, and total oxidative status on sarcopenia and frailty syndrome
title_sort aging and the impact of global dna methylation, telomere shortening, and total oxidative status on sarcopenia and frailty syndrome
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10644469/
https://www.ncbi.nlm.nih.gov/pubmed/37964387
http://dx.doi.org/10.1186/s12979-023-00384-2
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