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Single-cell sequencing reveals the reproductive variations between primiparous and multiparous Hu ewes
BACKGROUND: In the modern sheep production systems, the reproductive performance of ewes determines the economic profitability of farming. Revealing the genetic mechanisms underlying differences in the litter size is important for the selection and breeding of highly prolific ewes. Hu sheep, a high-...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10644470/ https://www.ncbi.nlm.nih.gov/pubmed/37964337 http://dx.doi.org/10.1186/s40104-023-00941-1 |
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author | Ge, Ting Wen, Yifan Li, Bo Huang, Xiaoyu Jiang, Shaohua Zhang, Enping |
author_facet | Ge, Ting Wen, Yifan Li, Bo Huang, Xiaoyu Jiang, Shaohua Zhang, Enping |
author_sort | Ge, Ting |
collection | PubMed |
description | BACKGROUND: In the modern sheep production systems, the reproductive performance of ewes determines the economic profitability of farming. Revealing the genetic mechanisms underlying differences in the litter size is important for the selection and breeding of highly prolific ewes. Hu sheep, a high-quality Chinese sheep breed, is known for its high fecundity and is often used as a model to study prolificacy traits. In the current study, animals were divided into two groups according to their delivery rates in three consecutive lambing seasons (namely, the high and low reproductive groups with ≥ 3 lambs and one lamb per season, n = 3, respectively). The ewes were slaughtered within 12 h of estrus, and unilateral ovarian tissues were collected and analyzed by 10× Genomics single-cell RNA sequencing. RESULTS: A total of 5 types of somatic cells were identified and corresponding expression profiles were mapped in the ovaries of each group. Noticeably, the differences in the ovary somatic cell expression profiles between the high and low reproductive groups were mainly clustered in the granulosa cells. Furthermore, four granulosa cell subtypes were identified. GeneSwitches analysis revealed that the abundance of JPH1 expression and the reduction of LOC101112291 expression could lead to different evolutionary directions of the granulosa cells. Additionally, the expression levels of FTH1 and FTL in mural granulosa cells of the highly reproductive group were significantly higher. These genes inhibit necroptosis and ferroptosis of mural granulosa cells, which helps prevent follicular atresia. CONCLUSIONS: This study provides insights into the molecular mechanisms underlying the high fecundity of Hu sheep. The differences in gene expression profiles, particularly in the granulosa cells, suggest that these cells play a critical role in female prolificacy. The findings also highlight the importance of genes such as JPH1, LOC101112291, FTH1, and FTL in regulating granulosa cell function and follicular development. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40104-023-00941-1. |
format | Online Article Text |
id | pubmed-10644470 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-106444702023-11-14 Single-cell sequencing reveals the reproductive variations between primiparous and multiparous Hu ewes Ge, Ting Wen, Yifan Li, Bo Huang, Xiaoyu Jiang, Shaohua Zhang, Enping J Anim Sci Biotechnol Research BACKGROUND: In the modern sheep production systems, the reproductive performance of ewes determines the economic profitability of farming. Revealing the genetic mechanisms underlying differences in the litter size is important for the selection and breeding of highly prolific ewes. Hu sheep, a high-quality Chinese sheep breed, is known for its high fecundity and is often used as a model to study prolificacy traits. In the current study, animals were divided into two groups according to their delivery rates in three consecutive lambing seasons (namely, the high and low reproductive groups with ≥ 3 lambs and one lamb per season, n = 3, respectively). The ewes were slaughtered within 12 h of estrus, and unilateral ovarian tissues were collected and analyzed by 10× Genomics single-cell RNA sequencing. RESULTS: A total of 5 types of somatic cells were identified and corresponding expression profiles were mapped in the ovaries of each group. Noticeably, the differences in the ovary somatic cell expression profiles between the high and low reproductive groups were mainly clustered in the granulosa cells. Furthermore, four granulosa cell subtypes were identified. GeneSwitches analysis revealed that the abundance of JPH1 expression and the reduction of LOC101112291 expression could lead to different evolutionary directions of the granulosa cells. Additionally, the expression levels of FTH1 and FTL in mural granulosa cells of the highly reproductive group were significantly higher. These genes inhibit necroptosis and ferroptosis of mural granulosa cells, which helps prevent follicular atresia. CONCLUSIONS: This study provides insights into the molecular mechanisms underlying the high fecundity of Hu sheep. The differences in gene expression profiles, particularly in the granulosa cells, suggest that these cells play a critical role in female prolificacy. The findings also highlight the importance of genes such as JPH1, LOC101112291, FTH1, and FTL in regulating granulosa cell function and follicular development. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40104-023-00941-1. BioMed Central 2023-11-14 /pmc/articles/PMC10644470/ /pubmed/37964337 http://dx.doi.org/10.1186/s40104-023-00941-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Ge, Ting Wen, Yifan Li, Bo Huang, Xiaoyu Jiang, Shaohua Zhang, Enping Single-cell sequencing reveals the reproductive variations between primiparous and multiparous Hu ewes |
title | Single-cell sequencing reveals the reproductive variations between primiparous and multiparous Hu ewes |
title_full | Single-cell sequencing reveals the reproductive variations between primiparous and multiparous Hu ewes |
title_fullStr | Single-cell sequencing reveals the reproductive variations between primiparous and multiparous Hu ewes |
title_full_unstemmed | Single-cell sequencing reveals the reproductive variations between primiparous and multiparous Hu ewes |
title_short | Single-cell sequencing reveals the reproductive variations between primiparous and multiparous Hu ewes |
title_sort | single-cell sequencing reveals the reproductive variations between primiparous and multiparous hu ewes |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10644470/ https://www.ncbi.nlm.nih.gov/pubmed/37964337 http://dx.doi.org/10.1186/s40104-023-00941-1 |
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