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Is the Urinary and Gut Microbiome Associated With Bladder Cancer?
BACKGROUND: Microbiome dysbiosis plays a role in the pathogenesis of many urological diseases, including bladder cancer (BC). The aim of the study was to compare the urinary and gut microbiota of patients with BC with a healthy control (HC) group. METHODS: The study group included patients hospitali...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10644734/ https://www.ncbi.nlm.nih.gov/pubmed/38023290 http://dx.doi.org/10.1177/11795549231206796 |
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author | Chorbińska, Joanna Krajewski, Wojciech Nowak, Łukasz Bardowska, Klaudia Żebrowska-Różańska, Paulina Łaczmański, Łukasz Pacyga-Prus, Katarzyna Górska, Sabina Małkiewicz, Bartosz Szydełko, Tomasz |
author_facet | Chorbińska, Joanna Krajewski, Wojciech Nowak, Łukasz Bardowska, Klaudia Żebrowska-Różańska, Paulina Łaczmański, Łukasz Pacyga-Prus, Katarzyna Górska, Sabina Małkiewicz, Bartosz Szydełko, Tomasz |
author_sort | Chorbińska, Joanna |
collection | PubMed |
description | BACKGROUND: Microbiome dysbiosis plays a role in the pathogenesis of many urological diseases, including bladder cancer (BC). The aim of the study was to compare the urinary and gut microbiota of patients with BC with a healthy control (HC) group. METHODS: The study group included patients hospitalized in 2020 to 2021 with diagnosed BC and HC. Prior to the transurethral resection of bladder tumor, patients collected their urine and stool which was then subjected to 16S rRNA gene sequencing. RESULTS: Overall, 25 patients were enrolled in the study: 18 in the BC group and 7 in the HC group. Analysis of the urine and stool microbiome showed no statistically significant differences between patients with BC and HC in alpha diversity, beta diversity, and difference in taxa relative abundance. Detailed analysis of urine and stool microbiome depending on patient- and tumor-related characteristics also showed no statistically significant differences in alpha diversity and beta diversity. Differences in abundance (ANCOM) were noted in both types of samples in patients with BC. In the urine test, genus Lactobacillus was more common in patients with a positive history of Bacillus Calmette–Guérin (BCG) therapy, while genus Howardella and the strain Streptococcus anginosus were more common in women. In stool samples, abundance of phylum Desulfobacterota was most abundant in Grade G1 and least in G2. Class Alphaproteobacteria, order Rhodospirillales, order Flavobacteriales, and family Flavobacteriaceae were more common in women. CONCLUSIONS: The microbiome of urine and stool of patients with BC does not differ significantly from that of HC; however, its composition in patients with BC varies according to the patient’s sex. |
format | Online Article Text |
id | pubmed-10644734 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-106447342023-11-13 Is the Urinary and Gut Microbiome Associated With Bladder Cancer? Chorbińska, Joanna Krajewski, Wojciech Nowak, Łukasz Bardowska, Klaudia Żebrowska-Różańska, Paulina Łaczmański, Łukasz Pacyga-Prus, Katarzyna Górska, Sabina Małkiewicz, Bartosz Szydełko, Tomasz Clin Med Insights Oncol Original Research Article BACKGROUND: Microbiome dysbiosis plays a role in the pathogenesis of many urological diseases, including bladder cancer (BC). The aim of the study was to compare the urinary and gut microbiota of patients with BC with a healthy control (HC) group. METHODS: The study group included patients hospitalized in 2020 to 2021 with diagnosed BC and HC. Prior to the transurethral resection of bladder tumor, patients collected their urine and stool which was then subjected to 16S rRNA gene sequencing. RESULTS: Overall, 25 patients were enrolled in the study: 18 in the BC group and 7 in the HC group. Analysis of the urine and stool microbiome showed no statistically significant differences between patients with BC and HC in alpha diversity, beta diversity, and difference in taxa relative abundance. Detailed analysis of urine and stool microbiome depending on patient- and tumor-related characteristics also showed no statistically significant differences in alpha diversity and beta diversity. Differences in abundance (ANCOM) were noted in both types of samples in patients with BC. In the urine test, genus Lactobacillus was more common in patients with a positive history of Bacillus Calmette–Guérin (BCG) therapy, while genus Howardella and the strain Streptococcus anginosus were more common in women. In stool samples, abundance of phylum Desulfobacterota was most abundant in Grade G1 and least in G2. Class Alphaproteobacteria, order Rhodospirillales, order Flavobacteriales, and family Flavobacteriaceae were more common in women. CONCLUSIONS: The microbiome of urine and stool of patients with BC does not differ significantly from that of HC; however, its composition in patients with BC varies according to the patient’s sex. SAGE Publications 2023-11-13 /pmc/articles/PMC10644734/ /pubmed/38023290 http://dx.doi.org/10.1177/11795549231206796 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Research Article Chorbińska, Joanna Krajewski, Wojciech Nowak, Łukasz Bardowska, Klaudia Żebrowska-Różańska, Paulina Łaczmański, Łukasz Pacyga-Prus, Katarzyna Górska, Sabina Małkiewicz, Bartosz Szydełko, Tomasz Is the Urinary and Gut Microbiome Associated With Bladder Cancer? |
title | Is the Urinary and Gut Microbiome Associated With Bladder Cancer? |
title_full | Is the Urinary and Gut Microbiome Associated With Bladder Cancer? |
title_fullStr | Is the Urinary and Gut Microbiome Associated With Bladder Cancer? |
title_full_unstemmed | Is the Urinary and Gut Microbiome Associated With Bladder Cancer? |
title_short | Is the Urinary and Gut Microbiome Associated With Bladder Cancer? |
title_sort | is the urinary and gut microbiome associated with bladder cancer? |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10644734/ https://www.ncbi.nlm.nih.gov/pubmed/38023290 http://dx.doi.org/10.1177/11795549231206796 |
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