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Intracytoplasmic sperm injection induces transgenerational abnormalities in mice
In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are 2 major assisted reproductive techniques (ARTs) used widely to treat infertility. Recently, spermatogonial transplantation emerged as a new ART to restore fertility to young patients with cancer after cancer therapy. To exa...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Clinical Investigation
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645388/ https://www.ncbi.nlm.nih.gov/pubmed/37966118 http://dx.doi.org/10.1172/JCI170140 |
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author | Kanatsu-Shinohara, Mito Shiromoto, Yusuke Ogonuki, Narumi Inoue, Kimiko Hattori, Satoko Miura, Kento Watanabe, Naomi Hasegawa, Ayumi Mochida, Keiji Yamamoto, Takuya Miyakawa, Tsuyoshi Ogura, Atsuo Shinohara, Takashi |
author_facet | Kanatsu-Shinohara, Mito Shiromoto, Yusuke Ogonuki, Narumi Inoue, Kimiko Hattori, Satoko Miura, Kento Watanabe, Naomi Hasegawa, Ayumi Mochida, Keiji Yamamoto, Takuya Miyakawa, Tsuyoshi Ogura, Atsuo Shinohara, Takashi |
author_sort | Kanatsu-Shinohara, Mito |
collection | PubMed |
description | In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are 2 major assisted reproductive techniques (ARTs) used widely to treat infertility. Recently, spermatogonial transplantation emerged as a new ART to restore fertility to young patients with cancer after cancer therapy. To examine the influence of germ cell manipulation on behavior of offspring, we produced F(1) offspring by a combination of two ARTs, spermatogonial transplantation and ICSI. When these animals were compared with F(1) offspring produced by ICSI using fresh wild-type sperm, not only spermatogonial transplantation–ICSI mice but also ICSI-only control mice exhibited behavioral abnormalities, which persisted in the F(2) generation. Furthermore, although these F(1) offspring appeared normal, F(2) offspring produced by IVF using F(1) sperm and wild-type oocytes showed various types of congenital abnormalities, including anophthalmia, hydrocephalus, and missing limbs. Therefore, ARTs can induce morphological and functional defects in mice, some of which become evident only after germline transmission. |
format | Online Article Text |
id | pubmed-10645388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Society for Clinical Investigation |
record_format | MEDLINE/PubMed |
spelling | pubmed-106453882023-11-15 Intracytoplasmic sperm injection induces transgenerational abnormalities in mice Kanatsu-Shinohara, Mito Shiromoto, Yusuke Ogonuki, Narumi Inoue, Kimiko Hattori, Satoko Miura, Kento Watanabe, Naomi Hasegawa, Ayumi Mochida, Keiji Yamamoto, Takuya Miyakawa, Tsuyoshi Ogura, Atsuo Shinohara, Takashi J Clin Invest Research Article In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are 2 major assisted reproductive techniques (ARTs) used widely to treat infertility. Recently, spermatogonial transplantation emerged as a new ART to restore fertility to young patients with cancer after cancer therapy. To examine the influence of germ cell manipulation on behavior of offspring, we produced F(1) offspring by a combination of two ARTs, spermatogonial transplantation and ICSI. When these animals were compared with F(1) offspring produced by ICSI using fresh wild-type sperm, not only spermatogonial transplantation–ICSI mice but also ICSI-only control mice exhibited behavioral abnormalities, which persisted in the F(2) generation. Furthermore, although these F(1) offspring appeared normal, F(2) offspring produced by IVF using F(1) sperm and wild-type oocytes showed various types of congenital abnormalities, including anophthalmia, hydrocephalus, and missing limbs. Therefore, ARTs can induce morphological and functional defects in mice, some of which become evident only after germline transmission. American Society for Clinical Investigation 2023-11-15 /pmc/articles/PMC10645388/ /pubmed/37966118 http://dx.doi.org/10.1172/JCI170140 Text en © 2023 Kanatsu-Shinohara et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Kanatsu-Shinohara, Mito Shiromoto, Yusuke Ogonuki, Narumi Inoue, Kimiko Hattori, Satoko Miura, Kento Watanabe, Naomi Hasegawa, Ayumi Mochida, Keiji Yamamoto, Takuya Miyakawa, Tsuyoshi Ogura, Atsuo Shinohara, Takashi Intracytoplasmic sperm injection induces transgenerational abnormalities in mice |
title | Intracytoplasmic sperm injection induces transgenerational abnormalities in mice |
title_full | Intracytoplasmic sperm injection induces transgenerational abnormalities in mice |
title_fullStr | Intracytoplasmic sperm injection induces transgenerational abnormalities in mice |
title_full_unstemmed | Intracytoplasmic sperm injection induces transgenerational abnormalities in mice |
title_short | Intracytoplasmic sperm injection induces transgenerational abnormalities in mice |
title_sort | intracytoplasmic sperm injection induces transgenerational abnormalities in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645388/ https://www.ncbi.nlm.nih.gov/pubmed/37966118 http://dx.doi.org/10.1172/JCI170140 |
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