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Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration
Improved materials for peripheral nerve repair are needed for the advancement of new surgical techniques in fields spanning from oncology to trauma. In this study, we developed bioresorbable materials capable of producing repeated electric field gradients spaced 600 μm apart to assess the impact on...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645611/ https://www.ncbi.nlm.nih.gov/pubmed/37964030 http://dx.doi.org/10.1007/s10856-023-06763-x |
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author | Rosenbalm, Tabitha N. Levi, Nicole H. Morykwas, Michael J. Wagner, William D. |
author_facet | Rosenbalm, Tabitha N. Levi, Nicole H. Morykwas, Michael J. Wagner, William D. |
author_sort | Rosenbalm, Tabitha N. |
collection | PubMed |
description | Improved materials for peripheral nerve repair are needed for the advancement of new surgical techniques in fields spanning from oncology to trauma. In this study, we developed bioresorbable materials capable of producing repeated electric field gradients spaced 600 μm apart to assess the impact on neuronal cell growth, and migration. Electrically conductive, biphasic composites comprised of poly (glycerol) sebacate acrylate (PGSA) alone, and doped with poly (pyrrole) (PPy), were prepared to create alternating segments with high and low electrically conductivity. Conductivity measurements demonstrated that 0.05% PPy added to PSA achieved an optimal value of 1.25 × 10(−4) S/cm, for subsequent electrical stimulation. Tensile testing and degradation of PPy doped and undoped PGSA determined that 35–40% acrylation of PGSA matched nerve mechanical properties. Both fibroblast and neuronal cells thrived when cultured upon the composite. Biphasic PGSA/PPy sheets seeded with neuronal cells stimulated for with 3 V, 20 Hz demonstrated a 5x cell increase with 1 day of stimulation and up to a 10x cell increase with 3 days stimulation compared to non-stimulated composites. Tubular conduits composed of repeated high and low conductivity materials suitable for implantation in the rat sciatic nerve model for nerve repair were evaluated in vivo and were superior to silicone conduits. These results suggest that biphasic conducting conduits capable of maintaining mechanical properties without inducing compression injuries while generating repeated electric fields are a promising tool for acceleration of peripheral nerve repair to previously untreatable patients. GRAPHICAL ABSTRACT: [Image: see text] |
format | Online Article Text |
id | pubmed-10645611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-106456112023-11-14 Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration Rosenbalm, Tabitha N. Levi, Nicole H. Morykwas, Michael J. Wagner, William D. J Mater Sci Mater Med Biomaterials Synthesis and Characterization Improved materials for peripheral nerve repair are needed for the advancement of new surgical techniques in fields spanning from oncology to trauma. In this study, we developed bioresorbable materials capable of producing repeated electric field gradients spaced 600 μm apart to assess the impact on neuronal cell growth, and migration. Electrically conductive, biphasic composites comprised of poly (glycerol) sebacate acrylate (PGSA) alone, and doped with poly (pyrrole) (PPy), were prepared to create alternating segments with high and low electrically conductivity. Conductivity measurements demonstrated that 0.05% PPy added to PSA achieved an optimal value of 1.25 × 10(−4) S/cm, for subsequent electrical stimulation. Tensile testing and degradation of PPy doped and undoped PGSA determined that 35–40% acrylation of PGSA matched nerve mechanical properties. Both fibroblast and neuronal cells thrived when cultured upon the composite. Biphasic PGSA/PPy sheets seeded with neuronal cells stimulated for with 3 V, 20 Hz demonstrated a 5x cell increase with 1 day of stimulation and up to a 10x cell increase with 3 days stimulation compared to non-stimulated composites. Tubular conduits composed of repeated high and low conductivity materials suitable for implantation in the rat sciatic nerve model for nerve repair were evaluated in vivo and were superior to silicone conduits. These results suggest that biphasic conducting conduits capable of maintaining mechanical properties without inducing compression injuries while generating repeated electric fields are a promising tool for acceleration of peripheral nerve repair to previously untreatable patients. GRAPHICAL ABSTRACT: [Image: see text] Springer US 2023-11-15 2023 /pmc/articles/PMC10645611/ /pubmed/37964030 http://dx.doi.org/10.1007/s10856-023-06763-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Biomaterials Synthesis and Characterization Rosenbalm, Tabitha N. Levi, Nicole H. Morykwas, Michael J. Wagner, William D. Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration |
title | Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration |
title_full | Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration |
title_fullStr | Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration |
title_full_unstemmed | Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration |
title_short | Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration |
title_sort | electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration |
topic | Biomaterials Synthesis and Characterization |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645611/ https://www.ncbi.nlm.nih.gov/pubmed/37964030 http://dx.doi.org/10.1007/s10856-023-06763-x |
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