Prevalence of the virulence genes and their correlation with carbapenem resistance amongst the Pseudomonas aeruginosa strains isolated from a tertiary hospital in China

Pseudomonas aeruginosa is one of the top-listed pathogens in nosocomial infection. It is notorious for its complicated virulence system and rapid adaptability to drugs or antimicrobials. In this study, we aimed to evaluate the prevalence of sixteen virulence genes in four groups including type III s...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Xiaohuan, Gao, Kaijing, Chen, Cuicui, Zhang, Cuiping, Zhou, Chunmei, Song, Yuanlin, Guo, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2023
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645663/
https://www.ncbi.nlm.nih.gov/pubmed/37847452
http://dx.doi.org/10.1007/s10482-023-01869-2
_version_ 1785147399690256384
author Wang, Xiaohuan
Gao, Kaijing
Chen, Cuicui
Zhang, Cuiping
Zhou, Chunmei
Song, Yuanlin
Guo, Wei
author_facet Wang, Xiaohuan
Gao, Kaijing
Chen, Cuicui
Zhang, Cuiping
Zhou, Chunmei
Song, Yuanlin
Guo, Wei
author_sort Wang, Xiaohuan
collection PubMed
description Pseudomonas aeruginosa is one of the top-listed pathogens in nosocomial infection. It is notorious for its complicated virulence system and rapid adaptability to drugs or antimicrobials. In this study, we aimed to evaluate the prevalence of sixteen virulence genes in four groups including type III secretion system, biofilm formation, extracellular toxin biosynthesis and enzymes amongst 209 clinical Pseudomonas aeruginosa strains. We investigated the different distribution patterns of virulence genotypes based on carbapenem-resistant phenotype or the carriage of carbapenemase genes. The detection rate of each virulence gene varied greatly. phzM and plcN were detected in all collected strains, while pilB and exoU were only carried by a small portion of isolates (6.7% and 16.3%). Additionally, the number of genotypes observed in each group of examined virulence genes ranged from 4 to 8. Only the distribution of genotypes of type III secretion system showed statistical difference between carbapenem-mediated or carbapenem-resistant and carbapenem-sensitive strains. The virulence genotype of Pseudomonas aeruginosa was possibly interrelated to its resistance mechanism. Further research suggested that one particular TTSS genotype exhibited higher ratio in carbapenemase-producing strains and exoS was less frequently detected in CRPA strains carrying carbapenemase gene. Generally, the significant genetic diversity of virulence genes amongst Pseudomonas aeruginosa strains was highlighted in this study. Specific TTSS genotypes were associated with carbapenem-resistance. In particular, certain incompatibility might exist between exoS and carbapenemase genes, which provided valuable information for further understanding the relationship between carbapenem resistance and virulence. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10482-023-01869-2.
format Online
Article
Text
id pubmed-10645663
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Springer International Publishing
record_format MEDLINE/PubMed
spelling pubmed-106456632023-11-14 Prevalence of the virulence genes and their correlation with carbapenem resistance amongst the Pseudomonas aeruginosa strains isolated from a tertiary hospital in China Wang, Xiaohuan Gao, Kaijing Chen, Cuicui Zhang, Cuiping Zhou, Chunmei Song, Yuanlin Guo, Wei Antonie Van Leeuwenhoek Original Paper Pseudomonas aeruginosa is one of the top-listed pathogens in nosocomial infection. It is notorious for its complicated virulence system and rapid adaptability to drugs or antimicrobials. In this study, we aimed to evaluate the prevalence of sixteen virulence genes in four groups including type III secretion system, biofilm formation, extracellular toxin biosynthesis and enzymes amongst 209 clinical Pseudomonas aeruginosa strains. We investigated the different distribution patterns of virulence genotypes based on carbapenem-resistant phenotype or the carriage of carbapenemase genes. The detection rate of each virulence gene varied greatly. phzM and plcN were detected in all collected strains, while pilB and exoU were only carried by a small portion of isolates (6.7% and 16.3%). Additionally, the number of genotypes observed in each group of examined virulence genes ranged from 4 to 8. Only the distribution of genotypes of type III secretion system showed statistical difference between carbapenem-mediated or carbapenem-resistant and carbapenem-sensitive strains. The virulence genotype of Pseudomonas aeruginosa was possibly interrelated to its resistance mechanism. Further research suggested that one particular TTSS genotype exhibited higher ratio in carbapenemase-producing strains and exoS was less frequently detected in CRPA strains carrying carbapenemase gene. Generally, the significant genetic diversity of virulence genes amongst Pseudomonas aeruginosa strains was highlighted in this study. Specific TTSS genotypes were associated with carbapenem-resistance. In particular, certain incompatibility might exist between exoS and carbapenemase genes, which provided valuable information for further understanding the relationship between carbapenem resistance and virulence. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10482-023-01869-2. Springer International Publishing 2023-10-17 2023 /pmc/articles/PMC10645663/ /pubmed/37847452 http://dx.doi.org/10.1007/s10482-023-01869-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Wang, Xiaohuan
Gao, Kaijing
Chen, Cuicui
Zhang, Cuiping
Zhou, Chunmei
Song, Yuanlin
Guo, Wei
Prevalence of the virulence genes and their correlation with carbapenem resistance amongst the Pseudomonas aeruginosa strains isolated from a tertiary hospital in China
title Prevalence of the virulence genes and their correlation with carbapenem resistance amongst the Pseudomonas aeruginosa strains isolated from a tertiary hospital in China
title_full Prevalence of the virulence genes and their correlation with carbapenem resistance amongst the Pseudomonas aeruginosa strains isolated from a tertiary hospital in China
title_fullStr Prevalence of the virulence genes and their correlation with carbapenem resistance amongst the Pseudomonas aeruginosa strains isolated from a tertiary hospital in China
title_full_unstemmed Prevalence of the virulence genes and their correlation with carbapenem resistance amongst the Pseudomonas aeruginosa strains isolated from a tertiary hospital in China
title_short Prevalence of the virulence genes and their correlation with carbapenem resistance amongst the Pseudomonas aeruginosa strains isolated from a tertiary hospital in China
title_sort prevalence of the virulence genes and their correlation with carbapenem resistance amongst the pseudomonas aeruginosa strains isolated from a tertiary hospital in china
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645663/
https://www.ncbi.nlm.nih.gov/pubmed/37847452
http://dx.doi.org/10.1007/s10482-023-01869-2
work_keys_str_mv AT wangxiaohuan prevalenceofthevirulencegenesandtheircorrelationwithcarbapenemresistanceamongstthepseudomonasaeruginosastrainsisolatedfromatertiaryhospitalinchina
AT gaokaijing prevalenceofthevirulencegenesandtheircorrelationwithcarbapenemresistanceamongstthepseudomonasaeruginosastrainsisolatedfromatertiaryhospitalinchina
AT chencuicui prevalenceofthevirulencegenesandtheircorrelationwithcarbapenemresistanceamongstthepseudomonasaeruginosastrainsisolatedfromatertiaryhospitalinchina
AT zhangcuiping prevalenceofthevirulencegenesandtheircorrelationwithcarbapenemresistanceamongstthepseudomonasaeruginosastrainsisolatedfromatertiaryhospitalinchina
AT zhouchunmei prevalenceofthevirulencegenesandtheircorrelationwithcarbapenemresistanceamongstthepseudomonasaeruginosastrainsisolatedfromatertiaryhospitalinchina
AT songyuanlin prevalenceofthevirulencegenesandtheircorrelationwithcarbapenemresistanceamongstthepseudomonasaeruginosastrainsisolatedfromatertiaryhospitalinchina
AT guowei prevalenceofthevirulencegenesandtheircorrelationwithcarbapenemresistanceamongstthepseudomonasaeruginosastrainsisolatedfromatertiaryhospitalinchina

Ejemplares similares