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Downregulation of RNA binding protein 47 predicts low survival in patients and promotes the development of renal cell malignancies through RNA stability modification

RNA binding proteins (RBPs) are crucial for cell function, tissue growth, and disease development in disease or normal physiological processes. RNA binding motif protein 47 (RBM47) has been proven to have anti-tumor effects on many cancers, but its effect is not yet clear in renal cancer. Here, we d...

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Autores principales: Wang, Cheng, Li, Weiquan, Meng, Xiangui, Yuan, Hongwei, Yu, Tiexi, Yang, Wei, Ni, Dong, Liu, Lei, Xiao, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Nature Singapore 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645769/
https://www.ncbi.nlm.nih.gov/pubmed/37962768
http://dx.doi.org/10.1186/s43556-023-00148-w
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author Wang, Cheng
Li, Weiquan
Meng, Xiangui
Yuan, Hongwei
Yu, Tiexi
Yang, Wei
Ni, Dong
Liu, Lei
Xiao, Wen
author_facet Wang, Cheng
Li, Weiquan
Meng, Xiangui
Yuan, Hongwei
Yu, Tiexi
Yang, Wei
Ni, Dong
Liu, Lei
Xiao, Wen
author_sort Wang, Cheng
collection PubMed
description RNA binding proteins (RBPs) are crucial for cell function, tissue growth, and disease development in disease or normal physiological processes. RNA binding motif protein 47 (RBM47) has been proven to have anti-tumor effects on many cancers, but its effect is not yet clear in renal cancer. Here, we demonstrated the expression and the prognostic role of RBM47 in public databases and clinical samples of clear cell renal carcinoma (ccRCC) with bioinformatics analysis. The possible mechanism of RBM47 in renal cancer was verified by gene function prediction and in vitro experiments. The results showed that RBM47 was downregulated in renal cancers when compared with control groups. Low RBM47 expression indicated poor prognosis in ccRCC. RBM47 expression in renal cancer cell lines was reduced significantly when compared to normal renal tubular epithelial cells. Epithelial-mesenchymal transition (EMT) and transforming growth factor-β signaling pathway was associated with RBM47 in ccRCC by Gene set enrichment analysis. RBM47 expression had a positive correlation with e-cadherin, but a negative correlation with snail and vimentin. RBM47 overexpression could repress the migration, invasion activity, and proliferation capacity of renal cancer cells, while RBM47 inhibition could promote the development of the malignant features through EMT signaling by RNA stability modification. Therefore, our results suggest that RBM47, as a new molecular biomarker, may play a key role in the cancer development of ccRCC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43556-023-00148-w.
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spelling pubmed-106457692023-11-14 Downregulation of RNA binding protein 47 predicts low survival in patients and promotes the development of renal cell malignancies through RNA stability modification Wang, Cheng Li, Weiquan Meng, Xiangui Yuan, Hongwei Yu, Tiexi Yang, Wei Ni, Dong Liu, Lei Xiao, Wen Mol Biomed Research RNA binding proteins (RBPs) are crucial for cell function, tissue growth, and disease development in disease or normal physiological processes. RNA binding motif protein 47 (RBM47) has been proven to have anti-tumor effects on many cancers, but its effect is not yet clear in renal cancer. Here, we demonstrated the expression and the prognostic role of RBM47 in public databases and clinical samples of clear cell renal carcinoma (ccRCC) with bioinformatics analysis. The possible mechanism of RBM47 in renal cancer was verified by gene function prediction and in vitro experiments. The results showed that RBM47 was downregulated in renal cancers when compared with control groups. Low RBM47 expression indicated poor prognosis in ccRCC. RBM47 expression in renal cancer cell lines was reduced significantly when compared to normal renal tubular epithelial cells. Epithelial-mesenchymal transition (EMT) and transforming growth factor-β signaling pathway was associated with RBM47 in ccRCC by Gene set enrichment analysis. RBM47 expression had a positive correlation with e-cadherin, but a negative correlation with snail and vimentin. RBM47 overexpression could repress the migration, invasion activity, and proliferation capacity of renal cancer cells, while RBM47 inhibition could promote the development of the malignant features through EMT signaling by RNA stability modification. Therefore, our results suggest that RBM47, as a new molecular biomarker, may play a key role in the cancer development of ccRCC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43556-023-00148-w. Springer Nature Singapore 2023-11-14 /pmc/articles/PMC10645769/ /pubmed/37962768 http://dx.doi.org/10.1186/s43556-023-00148-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Wang, Cheng
Li, Weiquan
Meng, Xiangui
Yuan, Hongwei
Yu, Tiexi
Yang, Wei
Ni, Dong
Liu, Lei
Xiao, Wen
Downregulation of RNA binding protein 47 predicts low survival in patients and promotes the development of renal cell malignancies through RNA stability modification
title Downregulation of RNA binding protein 47 predicts low survival in patients and promotes the development of renal cell malignancies through RNA stability modification
title_full Downregulation of RNA binding protein 47 predicts low survival in patients and promotes the development of renal cell malignancies through RNA stability modification
title_fullStr Downregulation of RNA binding protein 47 predicts low survival in patients and promotes the development of renal cell malignancies through RNA stability modification
title_full_unstemmed Downregulation of RNA binding protein 47 predicts low survival in patients and promotes the development of renal cell malignancies through RNA stability modification
title_short Downregulation of RNA binding protein 47 predicts low survival in patients and promotes the development of renal cell malignancies through RNA stability modification
title_sort downregulation of rna binding protein 47 predicts low survival in patients and promotes the development of renal cell malignancies through rna stability modification
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645769/
https://www.ncbi.nlm.nih.gov/pubmed/37962768
http://dx.doi.org/10.1186/s43556-023-00148-w
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