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Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment
A eukaryotic cell is a microscopic world within which efficient material transport is essential. Yet, how a cell manages to deliver cellular cargos efficiently in a crowded environment remains poorly understood. Here, we used interferometric scattering microscopy to track unlabeled cargos in directi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645962/ https://www.ncbi.nlm.nih.gov/pubmed/37963891 http://dx.doi.org/10.1038/s41467-023-42347-7 |
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author | Park, Jin-Sung Lee, Il-Buem Moon, Hyeon-Min Hong, Seok-Cheol Cho, Minhaeng |
author_facet | Park, Jin-Sung Lee, Il-Buem Moon, Hyeon-Min Hong, Seok-Cheol Cho, Minhaeng |
author_sort | Park, Jin-Sung |
collection | PubMed |
description | A eukaryotic cell is a microscopic world within which efficient material transport is essential. Yet, how a cell manages to deliver cellular cargos efficiently in a crowded environment remains poorly understood. Here, we used interferometric scattering microscopy to track unlabeled cargos in directional motion in a massively parallel fashion. Our label-free, cargo-tracing method revealed not only the dynamics of cargo transportation but also the fine architecture of the actively used cytoskeletal highways and the long-term evolution of the associated traffic at sub-diffraction resolution. Cargos frequently run into a blocked road or experience a traffic jam. Still, they have effective strategies to circumvent those problems: opting for an alternative mode of transport and moving together in tandem or migrating collectively. All taken together, a cell is an incredibly complex and busy space where the principle and practice of transportation intriguingly parallel those of our macroscopic world. |
format | Online Article Text |
id | pubmed-10645962 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106459622023-11-14 Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment Park, Jin-Sung Lee, Il-Buem Moon, Hyeon-Min Hong, Seok-Cheol Cho, Minhaeng Nat Commun Article A eukaryotic cell is a microscopic world within which efficient material transport is essential. Yet, how a cell manages to deliver cellular cargos efficiently in a crowded environment remains poorly understood. Here, we used interferometric scattering microscopy to track unlabeled cargos in directional motion in a massively parallel fashion. Our label-free, cargo-tracing method revealed not only the dynamics of cargo transportation but also the fine architecture of the actively used cytoskeletal highways and the long-term evolution of the associated traffic at sub-diffraction resolution. Cargos frequently run into a blocked road or experience a traffic jam. Still, they have effective strategies to circumvent those problems: opting for an alternative mode of transport and moving together in tandem or migrating collectively. All taken together, a cell is an incredibly complex and busy space where the principle and practice of transportation intriguingly parallel those of our macroscopic world. Nature Publishing Group UK 2023-11-14 /pmc/articles/PMC10645962/ /pubmed/37963891 http://dx.doi.org/10.1038/s41467-023-42347-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Park, Jin-Sung Lee, Il-Buem Moon, Hyeon-Min Hong, Seok-Cheol Cho, Minhaeng Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment |
title | Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment |
title_full | Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment |
title_fullStr | Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment |
title_full_unstemmed | Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment |
title_short | Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment |
title_sort | long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645962/ https://www.ncbi.nlm.nih.gov/pubmed/37963891 http://dx.doi.org/10.1038/s41467-023-42347-7 |
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