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Salicylate- and Noise-induced Tinnitus. Different Mechanisms Producing the same Result? An Experimental Model
Purpose: Tinnitus, the generation of phantom sounds, can be the result of noise exposure, however, understanding of its underlying mechanisms is limited. Purpose of the study was is to determine whether different concentrations of salicylate can cause tinnitus of different intensity. Methods: For th...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer India
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10646040/ https://www.ncbi.nlm.nih.gov/pubmed/38027533 http://dx.doi.org/10.1007/s12070-023-04049-w |
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author | Pavlidis, Pavlos Papadopoulou, Kyriaki Tseriotis, Vasilis Spyridon Karachrysafi, Sophia Sardeli, Chrysanthi Gouveris, Haralampos Papamitsou, Theodora Sioga, Antonia Kouvelas, Dimitrios |
author_facet | Pavlidis, Pavlos Papadopoulou, Kyriaki Tseriotis, Vasilis Spyridon Karachrysafi, Sophia Sardeli, Chrysanthi Gouveris, Haralampos Papamitsou, Theodora Sioga, Antonia Kouvelas, Dimitrios |
author_sort | Pavlidis, Pavlos |
collection | PubMed |
description | Purpose: Tinnitus, the generation of phantom sounds, can be the result of noise exposure, however, understanding of its underlying mechanisms is limited. Purpose of the study was is to determine whether different concentrations of salicylate can cause tinnitus of different intensity. Methods: For the purposes of this study 50 male Wistar rats were used. The animals were divided into 5 groups (10 rats in each group). The animals that did not receive any substance were allocated to the control group (Group A). The second group (Group B) of rats received salicylate (Sigma Aldrich) intraperitoneally for 7 days (300 mg/Kg/day). The 3rd group (Group C) received salicylate intraperitoneally for 7 days, but at twice the concentration of the animals in the second group (600 mg/kg/d). The 4th group (Group D) simultaneously received salicylate (300 mg/Kg/day) and pure Memantine (Sigma Aldrich, 10 mg/kg/d) intraperitoneally for 7 days. The 5th group (Group E) did not receive any substance but was exposed for 168 consecutive hours (7 days) to sound to induce tinnitus. Cochlear activity was evaluated with the use of Distortion Product Otoacoustic Emissions (DPOAEs). At the end of the experimental period, the animals were sacrificed, and the right cochlea was removed and prepared for further histological and immunohistochemical studies. Results: The DPOAEs of animals treated either with salicylate as monotherapy or salicylate combined with memantine were indistinguishable from the noise floor, did not differ significantly compared to the animals of the control group or those expose to constant noise. The cochlear structures of Group E remained anatomically and functionally unaffected from the exposure to constant noise. Memantine does not seem to offer substantial protection to the cochlear structures, according to histological examination and hearing tests, however, the rats receiving it exhibited better results in behavioral tests. Conclusions: The administration of memantine does not contribute significantly to the reduction of tinnitus. |
format | Online Article Text |
id | pubmed-10646040 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer India |
record_format | MEDLINE/PubMed |
spelling | pubmed-106460402023-07-04 Salicylate- and Noise-induced Tinnitus. Different Mechanisms Producing the same Result? An Experimental Model Pavlidis, Pavlos Papadopoulou, Kyriaki Tseriotis, Vasilis Spyridon Karachrysafi, Sophia Sardeli, Chrysanthi Gouveris, Haralampos Papamitsou, Theodora Sioga, Antonia Kouvelas, Dimitrios Indian J Otolaryngol Head Neck Surg Original Article Purpose: Tinnitus, the generation of phantom sounds, can be the result of noise exposure, however, understanding of its underlying mechanisms is limited. Purpose of the study was is to determine whether different concentrations of salicylate can cause tinnitus of different intensity. Methods: For the purposes of this study 50 male Wistar rats were used. The animals were divided into 5 groups (10 rats in each group). The animals that did not receive any substance were allocated to the control group (Group A). The second group (Group B) of rats received salicylate (Sigma Aldrich) intraperitoneally for 7 days (300 mg/Kg/day). The 3rd group (Group C) received salicylate intraperitoneally for 7 days, but at twice the concentration of the animals in the second group (600 mg/kg/d). The 4th group (Group D) simultaneously received salicylate (300 mg/Kg/day) and pure Memantine (Sigma Aldrich, 10 mg/kg/d) intraperitoneally for 7 days. The 5th group (Group E) did not receive any substance but was exposed for 168 consecutive hours (7 days) to sound to induce tinnitus. Cochlear activity was evaluated with the use of Distortion Product Otoacoustic Emissions (DPOAEs). At the end of the experimental period, the animals were sacrificed, and the right cochlea was removed and prepared for further histological and immunohistochemical studies. Results: The DPOAEs of animals treated either with salicylate as monotherapy or salicylate combined with memantine were indistinguishable from the noise floor, did not differ significantly compared to the animals of the control group or those expose to constant noise. The cochlear structures of Group E remained anatomically and functionally unaffected from the exposure to constant noise. Memantine does not seem to offer substantial protection to the cochlear structures, according to histological examination and hearing tests, however, the rats receiving it exhibited better results in behavioral tests. Conclusions: The administration of memantine does not contribute significantly to the reduction of tinnitus. Springer India 2023-07-04 2023-12 /pmc/articles/PMC10646040/ /pubmed/38027533 http://dx.doi.org/10.1007/s12070-023-04049-w Text en © The Author(s) 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Pavlidis, Pavlos Papadopoulou, Kyriaki Tseriotis, Vasilis Spyridon Karachrysafi, Sophia Sardeli, Chrysanthi Gouveris, Haralampos Papamitsou, Theodora Sioga, Antonia Kouvelas, Dimitrios Salicylate- and Noise-induced Tinnitus. Different Mechanisms Producing the same Result? An Experimental Model |
title | Salicylate- and Noise-induced Tinnitus. Different Mechanisms Producing the same Result? An Experimental Model |
title_full | Salicylate- and Noise-induced Tinnitus. Different Mechanisms Producing the same Result? An Experimental Model |
title_fullStr | Salicylate- and Noise-induced Tinnitus. Different Mechanisms Producing the same Result? An Experimental Model |
title_full_unstemmed | Salicylate- and Noise-induced Tinnitus. Different Mechanisms Producing the same Result? An Experimental Model |
title_short | Salicylate- and Noise-induced Tinnitus. Different Mechanisms Producing the same Result? An Experimental Model |
title_sort | salicylate- and noise-induced tinnitus. different mechanisms producing the same result? an experimental model |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10646040/ https://www.ncbi.nlm.nih.gov/pubmed/38027533 http://dx.doi.org/10.1007/s12070-023-04049-w |
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