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The expanding range of emerging tick-borne viruses in Eastern Europe and the Black Sea Region
We analysed both pooled and individual tick samples collected from four countries in Eastern Europe and the Black Sea region, using metagenome-based nanopore sequencing (NS) and targeted amplification. Initially, 1337 ticks, belonging to 11 species, were screened in 217 pools. Viruses (21 taxa) and...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10646066/ https://www.ncbi.nlm.nih.gov/pubmed/37963929 http://dx.doi.org/10.1038/s41598-023-46879-2 |
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author | Ergunay, Koray Bourke, Brian P. Reinbold-Wasson, Drew D. Nikolich, Mikeljon P. Nelson, Suppaluck P. Caicedo-Quiroga, Laura Vaydayko, Nataliya Kirkitadze, Giorgi Chunashvili, Tamar Long, Lewis S. Blackburn, Jason K. Cleary, Nora G. Tucker, Cynthia L. Linton, Yvonne-Marie |
author_facet | Ergunay, Koray Bourke, Brian P. Reinbold-Wasson, Drew D. Nikolich, Mikeljon P. Nelson, Suppaluck P. Caicedo-Quiroga, Laura Vaydayko, Nataliya Kirkitadze, Giorgi Chunashvili, Tamar Long, Lewis S. Blackburn, Jason K. Cleary, Nora G. Tucker, Cynthia L. Linton, Yvonne-Marie |
author_sort | Ergunay, Koray |
collection | PubMed |
description | We analysed both pooled and individual tick samples collected from four countries in Eastern Europe and the Black Sea region, using metagenome-based nanopore sequencing (NS) and targeted amplification. Initially, 1337 ticks, belonging to 11 species, were screened in 217 pools. Viruses (21 taxa) and human pathogens were detected in 46.5% and 7.3%, respectively. Tick-borne viral pathogens comprised Tacheng Tick Virus 2 (TTV2, 5.9%), Jingmen Tick Virus (JMTV, 0.9%) and Tacheng Tick Virus 1 (TTV1, 0.4%). An association of tick species with individual virus taxa was observed, with the exception of TTV2, which was observed in both Dermacentor and Haemaphysalis species. Individual ticks from pools with pathogen detection were then further screened by targeted amplification and then NS, which provided extensive genome data and revealed probable pathogen Haseki Tick Virus (HTV, 10.2%). Two distinct TTV2 clades were observed in phylogenetic analysis, one of which included closely related Dermacentor reticulatus Uukuviruses. JMTV detection indicated integrated virus sequences. Overall, we observed an expansion of newly documented pathogenic tick-borne viruses into Europe, with TTV1 being identified on the continent for the first time. These viruses should be included in the diagnostic assessment of symptomatic cases associated with tick bites and vector surveillance efforts. NS is shown as a useful tool for monitoring tick-associated pathogens in pooled or individual samples. |
format | Online Article Text |
id | pubmed-10646066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106460662023-11-14 The expanding range of emerging tick-borne viruses in Eastern Europe and the Black Sea Region Ergunay, Koray Bourke, Brian P. Reinbold-Wasson, Drew D. Nikolich, Mikeljon P. Nelson, Suppaluck P. Caicedo-Quiroga, Laura Vaydayko, Nataliya Kirkitadze, Giorgi Chunashvili, Tamar Long, Lewis S. Blackburn, Jason K. Cleary, Nora G. Tucker, Cynthia L. Linton, Yvonne-Marie Sci Rep Article We analysed both pooled and individual tick samples collected from four countries in Eastern Europe and the Black Sea region, using metagenome-based nanopore sequencing (NS) and targeted amplification. Initially, 1337 ticks, belonging to 11 species, were screened in 217 pools. Viruses (21 taxa) and human pathogens were detected in 46.5% and 7.3%, respectively. Tick-borne viral pathogens comprised Tacheng Tick Virus 2 (TTV2, 5.9%), Jingmen Tick Virus (JMTV, 0.9%) and Tacheng Tick Virus 1 (TTV1, 0.4%). An association of tick species with individual virus taxa was observed, with the exception of TTV2, which was observed in both Dermacentor and Haemaphysalis species. Individual ticks from pools with pathogen detection were then further screened by targeted amplification and then NS, which provided extensive genome data and revealed probable pathogen Haseki Tick Virus (HTV, 10.2%). Two distinct TTV2 clades were observed in phylogenetic analysis, one of which included closely related Dermacentor reticulatus Uukuviruses. JMTV detection indicated integrated virus sequences. Overall, we observed an expansion of newly documented pathogenic tick-borne viruses into Europe, with TTV1 being identified on the continent for the first time. These viruses should be included in the diagnostic assessment of symptomatic cases associated with tick bites and vector surveillance efforts. NS is shown as a useful tool for monitoring tick-associated pathogens in pooled or individual samples. Nature Publishing Group UK 2023-11-14 /pmc/articles/PMC10646066/ /pubmed/37963929 http://dx.doi.org/10.1038/s41598-023-46879-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Ergunay, Koray Bourke, Brian P. Reinbold-Wasson, Drew D. Nikolich, Mikeljon P. Nelson, Suppaluck P. Caicedo-Quiroga, Laura Vaydayko, Nataliya Kirkitadze, Giorgi Chunashvili, Tamar Long, Lewis S. Blackburn, Jason K. Cleary, Nora G. Tucker, Cynthia L. Linton, Yvonne-Marie The expanding range of emerging tick-borne viruses in Eastern Europe and the Black Sea Region |
title | The expanding range of emerging tick-borne viruses in Eastern Europe and the Black Sea Region |
title_full | The expanding range of emerging tick-borne viruses in Eastern Europe and the Black Sea Region |
title_fullStr | The expanding range of emerging tick-borne viruses in Eastern Europe and the Black Sea Region |
title_full_unstemmed | The expanding range of emerging tick-borne viruses in Eastern Europe and the Black Sea Region |
title_short | The expanding range of emerging tick-borne viruses in Eastern Europe and the Black Sea Region |
title_sort | expanding range of emerging tick-borne viruses in eastern europe and the black sea region |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10646066/ https://www.ncbi.nlm.nih.gov/pubmed/37963929 http://dx.doi.org/10.1038/s41598-023-46879-2 |
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