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Molecular diagnostic assay for pre-harvest detection of Tilletia indica infection in wheat plants

The current study describes a new diagnostic method for the rapid and accurate detection of Tilletia indica, the pathogen accountable for causing Karnal bunt (KB) disease in wheat. This method uses quantitative real-time polymerase chain reaction (qPCR) and a primer set derived from glyceraldehyde 3...

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Autores principales: Kashyap, Prem Lal, Kumar, Sudheer, Kumar, Ravi Shekhar, Sharma, Anju, Khanna, Annie, Raj, Shubham, Jasrotia, Poonam, Singh, Gyanendra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10646428/
https://www.ncbi.nlm.nih.gov/pubmed/38029161
http://dx.doi.org/10.3389/fmicb.2023.1291000
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author Kashyap, Prem Lal
Kumar, Sudheer
Kumar, Ravi Shekhar
Sharma, Anju
Khanna, Annie
Raj, Shubham
Jasrotia, Poonam
Singh, Gyanendra
author_facet Kashyap, Prem Lal
Kumar, Sudheer
Kumar, Ravi Shekhar
Sharma, Anju
Khanna, Annie
Raj, Shubham
Jasrotia, Poonam
Singh, Gyanendra
author_sort Kashyap, Prem Lal
collection PubMed
description The current study describes a new diagnostic method for the rapid and accurate detection of Tilletia indica, the pathogen accountable for causing Karnal bunt (KB) disease in wheat. This method uses quantitative real-time polymerase chain reaction (qPCR) and a primer set derived from glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene of T. indica to identify the presence of the pathogen. The qPCR assay using this primer set was found highly sensitive, with a limit of detection (LOD) value of 4 pg of T. indica DNA. This level of sensitivity allows for the detection of the pathogen even in cases of different growth stages of wheat, where no visible symptoms of infection on the wheat plants can be seen by naked eyes. The study also validated the qPCR assay on ten different wheat cultivars. Overall, this study presents a valuable molecular tool for rapid, specific and sensitive detection of KB fungus in wheat host. This method has practical applications in disease management, screening of wheat genotypes against KB and can aid in the development of strategies to mitigate the impact of Karnal bunt disease on wheat production.
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spelling pubmed-106464282023-11-01 Molecular diagnostic assay for pre-harvest detection of Tilletia indica infection in wheat plants Kashyap, Prem Lal Kumar, Sudheer Kumar, Ravi Shekhar Sharma, Anju Khanna, Annie Raj, Shubham Jasrotia, Poonam Singh, Gyanendra Front Microbiol Microbiology The current study describes a new diagnostic method for the rapid and accurate detection of Tilletia indica, the pathogen accountable for causing Karnal bunt (KB) disease in wheat. This method uses quantitative real-time polymerase chain reaction (qPCR) and a primer set derived from glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene of T. indica to identify the presence of the pathogen. The qPCR assay using this primer set was found highly sensitive, with a limit of detection (LOD) value of 4 pg of T. indica DNA. This level of sensitivity allows for the detection of the pathogen even in cases of different growth stages of wheat, where no visible symptoms of infection on the wheat plants can be seen by naked eyes. The study also validated the qPCR assay on ten different wheat cultivars. Overall, this study presents a valuable molecular tool for rapid, specific and sensitive detection of KB fungus in wheat host. This method has practical applications in disease management, screening of wheat genotypes against KB and can aid in the development of strategies to mitigate the impact of Karnal bunt disease on wheat production. Frontiers Media S.A. 2023-11-01 /pmc/articles/PMC10646428/ /pubmed/38029161 http://dx.doi.org/10.3389/fmicb.2023.1291000 Text en Copyright © 2023 Kashyap, Kumar, Kumar, Sharma, Khanna, Raj, Jasrotia and Singh. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Kashyap, Prem Lal
Kumar, Sudheer
Kumar, Ravi Shekhar
Sharma, Anju
Khanna, Annie
Raj, Shubham
Jasrotia, Poonam
Singh, Gyanendra
Molecular diagnostic assay for pre-harvest detection of Tilletia indica infection in wheat plants
title Molecular diagnostic assay for pre-harvest detection of Tilletia indica infection in wheat plants
title_full Molecular diagnostic assay for pre-harvest detection of Tilletia indica infection in wheat plants
title_fullStr Molecular diagnostic assay for pre-harvest detection of Tilletia indica infection in wheat plants
title_full_unstemmed Molecular diagnostic assay for pre-harvest detection of Tilletia indica infection in wheat plants
title_short Molecular diagnostic assay for pre-harvest detection of Tilletia indica infection in wheat plants
title_sort molecular diagnostic assay for pre-harvest detection of tilletia indica infection in wheat plants
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10646428/
https://www.ncbi.nlm.nih.gov/pubmed/38029161
http://dx.doi.org/10.3389/fmicb.2023.1291000
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