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Tetracalcium Phosphate Biocement Hardened with a Mixture of Phytic Acid–Phytase in the Healing Process of Osteochondral Defects in Sheep

Hyaline articular cartilage has unique physiological, biological, and biomechanical properties with very limited self-healing ability, which makes the process of cartilage regeneration extremely difficult. Therefore, research is currently focused on finding new and potentially better treatment optio...

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Autores principales: Varga, Maros, Kresakova, Lenka, Danko, Jan, Vdoviakova, Katarina, Humenik, Filip, Rusnak, Pavol, Giretova, Maria, Spakovska, Tatiana, Andrejcakova, Zuzana, Kadasi, Marian, Vrzgula, Marko, Criepokova, Zuzana, Ivaskova, Sonja, Korim, Filip, Medvecky, Lubomir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10647259/
https://www.ncbi.nlm.nih.gov/pubmed/37958674
http://dx.doi.org/10.3390/ijms242115690
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author Varga, Maros
Kresakova, Lenka
Danko, Jan
Vdoviakova, Katarina
Humenik, Filip
Rusnak, Pavol
Giretova, Maria
Spakovska, Tatiana
Andrejcakova, Zuzana
Kadasi, Marian
Vrzgula, Marko
Criepokova, Zuzana
Ivaskova, Sonja
Korim, Filip
Medvecky, Lubomir
author_facet Varga, Maros
Kresakova, Lenka
Danko, Jan
Vdoviakova, Katarina
Humenik, Filip
Rusnak, Pavol
Giretova, Maria
Spakovska, Tatiana
Andrejcakova, Zuzana
Kadasi, Marian
Vrzgula, Marko
Criepokova, Zuzana
Ivaskova, Sonja
Korim, Filip
Medvecky, Lubomir
author_sort Varga, Maros
collection PubMed
description Hyaline articular cartilage has unique physiological, biological, and biomechanical properties with very limited self-healing ability, which makes the process of cartilage regeneration extremely difficult. Therefore, research is currently focused on finding new and potentially better treatment options. The main objective of this in vivo study was to evaluate a novel biocement CX consisting of tetracalcium phosphate–monetit biocement hardened with a phytic acid–phytase mixture for the regeneration of osteochondral defects in sheep. The results were compared with tetracalcium phosphate–monetit biocement with classic fast-setting cement systems and untreated defects. After 6 months, the animals were sacrificed, and the samples were evaluated using macroscopic and histologic methods as well as X-ray, CT, and MR-imaging techniques. In contrast to the formation of fibrous or fibrocartilaginous tissue on the untreated side, treatment with biocements resulted in the formation of tissue with a dominant hyaline cartilage structure, although fine fibres were present (p < 0.001). There were no signs of pathomorphological changes or inflammation. Continuous formation of subchondral bone and hyaline cartilage layers was present even though residual biocement was observed in the trabecular bone. We consider biocement CX to be highly biocompatible and suitable for the treatment of osteochondral defects.
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spelling pubmed-106472592023-10-28 Tetracalcium Phosphate Biocement Hardened with a Mixture of Phytic Acid–Phytase in the Healing Process of Osteochondral Defects in Sheep Varga, Maros Kresakova, Lenka Danko, Jan Vdoviakova, Katarina Humenik, Filip Rusnak, Pavol Giretova, Maria Spakovska, Tatiana Andrejcakova, Zuzana Kadasi, Marian Vrzgula, Marko Criepokova, Zuzana Ivaskova, Sonja Korim, Filip Medvecky, Lubomir Int J Mol Sci Article Hyaline articular cartilage has unique physiological, biological, and biomechanical properties with very limited self-healing ability, which makes the process of cartilage regeneration extremely difficult. Therefore, research is currently focused on finding new and potentially better treatment options. The main objective of this in vivo study was to evaluate a novel biocement CX consisting of tetracalcium phosphate–monetit biocement hardened with a phytic acid–phytase mixture for the regeneration of osteochondral defects in sheep. The results were compared with tetracalcium phosphate–monetit biocement with classic fast-setting cement systems and untreated defects. After 6 months, the animals were sacrificed, and the samples were evaluated using macroscopic and histologic methods as well as X-ray, CT, and MR-imaging techniques. In contrast to the formation of fibrous or fibrocartilaginous tissue on the untreated side, treatment with biocements resulted in the formation of tissue with a dominant hyaline cartilage structure, although fine fibres were present (p < 0.001). There were no signs of pathomorphological changes or inflammation. Continuous formation of subchondral bone and hyaline cartilage layers was present even though residual biocement was observed in the trabecular bone. We consider biocement CX to be highly biocompatible and suitable for the treatment of osteochondral defects. MDPI 2023-10-28 /pmc/articles/PMC10647259/ /pubmed/37958674 http://dx.doi.org/10.3390/ijms242115690 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Varga, Maros
Kresakova, Lenka
Danko, Jan
Vdoviakova, Katarina
Humenik, Filip
Rusnak, Pavol
Giretova, Maria
Spakovska, Tatiana
Andrejcakova, Zuzana
Kadasi, Marian
Vrzgula, Marko
Criepokova, Zuzana
Ivaskova, Sonja
Korim, Filip
Medvecky, Lubomir
Tetracalcium Phosphate Biocement Hardened with a Mixture of Phytic Acid–Phytase in the Healing Process of Osteochondral Defects in Sheep
title Tetracalcium Phosphate Biocement Hardened with a Mixture of Phytic Acid–Phytase in the Healing Process of Osteochondral Defects in Sheep
title_full Tetracalcium Phosphate Biocement Hardened with a Mixture of Phytic Acid–Phytase in the Healing Process of Osteochondral Defects in Sheep
title_fullStr Tetracalcium Phosphate Biocement Hardened with a Mixture of Phytic Acid–Phytase in the Healing Process of Osteochondral Defects in Sheep
title_full_unstemmed Tetracalcium Phosphate Biocement Hardened with a Mixture of Phytic Acid–Phytase in the Healing Process of Osteochondral Defects in Sheep
title_short Tetracalcium Phosphate Biocement Hardened with a Mixture of Phytic Acid–Phytase in the Healing Process of Osteochondral Defects in Sheep
title_sort tetracalcium phosphate biocement hardened with a mixture of phytic acid–phytase in the healing process of osteochondral defects in sheep
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10647259/
https://www.ncbi.nlm.nih.gov/pubmed/37958674
http://dx.doi.org/10.3390/ijms242115690
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