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Influence of Isoflavones and Probiotics on Magnesium Status in Healthy Female Rats

Isoflavones and probiotics are promising nutrients for bone health, and magnesium (Mg) is essential for bone metabolism. This study aimed to determine the effects of daidzein, genistein and Lactobacillus acidophilus on the Mg status of healthy female rats. Forty-eight rats were randomly assigned to...

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Autores principales: Harahap, Iskandar Azmy, Kuligowski, Maciej, Schmidt, Marcin, Kurzawa, Paweł, Suliburska, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10647356/
https://www.ncbi.nlm.nih.gov/pubmed/37959026
http://dx.doi.org/10.3390/foods12213908
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author Harahap, Iskandar Azmy
Kuligowski, Maciej
Schmidt, Marcin
Kurzawa, Paweł
Suliburska, Joanna
author_facet Harahap, Iskandar Azmy
Kuligowski, Maciej
Schmidt, Marcin
Kurzawa, Paweł
Suliburska, Joanna
author_sort Harahap, Iskandar Azmy
collection PubMed
description Isoflavones and probiotics are promising nutrients for bone health, and magnesium (Mg) is essential for bone metabolism. This study aimed to determine the effects of daidzein, genistein and Lactobacillus acidophilus on the Mg status of healthy female rats. Forty-eight rats were randomly assigned to six groups, with the control group receiving a standard diet (AIN 93M). The remaining groups were fed the same diet with added ingredients such as tempeh flour; soy flour; pure daidzein and genistein; L. acidophilus or a combination of daidzein, genistein, and L. acidophilus. Tissue samples were collected after the eight-week intervention, and Mg concentrations were analysed using flame atomic absorption spectrometry. Myeloid and erythroid cells were determined using the haematoxylin and eosin bone staining method. Statistical analyses were conducted using one-way ANOVA with Tukey’s post hoc test and Pearson’s correlation coefficient. The threshold for significance was p < 0.05. Compared with the control group, adding tempeh to the diet of rats resulted in significant changes in Mg concentrations in various tissues, with a decrease in the kidneys and an increase in the fur. Although not statistically significant compared to the control group, the tempeh group showed increased Mg concentrations in the femur and spleen. The myeloid-to-erythroid cell ratio did not differ significantly among groups, but all intervention groups showed higher ratios than the control group. A strong negative correlation was observed between Mg concentrations in the kidneys and fur. Conversely, a positive correlation was identified between Mg concentrations in the pancreas and fur. Daily consumption of tempeh may improve Mg status in the organism. Intake of pure daidzein, genistein, or probiotic seems to have no effect on Mg concentrations in healthy rats.
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spelling pubmed-106473562023-10-25 Influence of Isoflavones and Probiotics on Magnesium Status in Healthy Female Rats Harahap, Iskandar Azmy Kuligowski, Maciej Schmidt, Marcin Kurzawa, Paweł Suliburska, Joanna Foods Article Isoflavones and probiotics are promising nutrients for bone health, and magnesium (Mg) is essential for bone metabolism. This study aimed to determine the effects of daidzein, genistein and Lactobacillus acidophilus on the Mg status of healthy female rats. Forty-eight rats were randomly assigned to six groups, with the control group receiving a standard diet (AIN 93M). The remaining groups were fed the same diet with added ingredients such as tempeh flour; soy flour; pure daidzein and genistein; L. acidophilus or a combination of daidzein, genistein, and L. acidophilus. Tissue samples were collected after the eight-week intervention, and Mg concentrations were analysed using flame atomic absorption spectrometry. Myeloid and erythroid cells were determined using the haematoxylin and eosin bone staining method. Statistical analyses were conducted using one-way ANOVA with Tukey’s post hoc test and Pearson’s correlation coefficient. The threshold for significance was p < 0.05. Compared with the control group, adding tempeh to the diet of rats resulted in significant changes in Mg concentrations in various tissues, with a decrease in the kidneys and an increase in the fur. Although not statistically significant compared to the control group, the tempeh group showed increased Mg concentrations in the femur and spleen. The myeloid-to-erythroid cell ratio did not differ significantly among groups, but all intervention groups showed higher ratios than the control group. A strong negative correlation was observed between Mg concentrations in the kidneys and fur. Conversely, a positive correlation was identified between Mg concentrations in the pancreas and fur. Daily consumption of tempeh may improve Mg status in the organism. Intake of pure daidzein, genistein, or probiotic seems to have no effect on Mg concentrations in healthy rats. MDPI 2023-10-25 /pmc/articles/PMC10647356/ /pubmed/37959026 http://dx.doi.org/10.3390/foods12213908 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Harahap, Iskandar Azmy
Kuligowski, Maciej
Schmidt, Marcin
Kurzawa, Paweł
Suliburska, Joanna
Influence of Isoflavones and Probiotics on Magnesium Status in Healthy Female Rats
title Influence of Isoflavones and Probiotics on Magnesium Status in Healthy Female Rats
title_full Influence of Isoflavones and Probiotics on Magnesium Status in Healthy Female Rats
title_fullStr Influence of Isoflavones and Probiotics on Magnesium Status in Healthy Female Rats
title_full_unstemmed Influence of Isoflavones and Probiotics on Magnesium Status in Healthy Female Rats
title_short Influence of Isoflavones and Probiotics on Magnesium Status in Healthy Female Rats
title_sort influence of isoflavones and probiotics on magnesium status in healthy female rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10647356/
https://www.ncbi.nlm.nih.gov/pubmed/37959026
http://dx.doi.org/10.3390/foods12213908
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