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Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration

Growth and development of the Ceratopteris hermaphroditic gametophytes are dependent on cell proliferation in the marginal meristem, which when destroyed will regenerate at a new location on the body margin. We established a laser ablation method to destroy a single initial cell in the meristem. Abl...

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Autores principales: Withers, Kelley A., Kvamme, Athena, Youngstrom, Christopher E., Yarvis, Rebekah M., Orpano, Rachel, Simons, Gabriel P., Irish, Erin E., Cheng, Chi-Lien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10647518/
https://www.ncbi.nlm.nih.gov/pubmed/37958815
http://dx.doi.org/10.3390/ijms242115832
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author Withers, Kelley A.
Kvamme, Athena
Youngstrom, Christopher E.
Yarvis, Rebekah M.
Orpano, Rachel
Simons, Gabriel P.
Irish, Erin E.
Cheng, Chi-Lien
author_facet Withers, Kelley A.
Kvamme, Athena
Youngstrom, Christopher E.
Yarvis, Rebekah M.
Orpano, Rachel
Simons, Gabriel P.
Irish, Erin E.
Cheng, Chi-Lien
author_sort Withers, Kelley A.
collection PubMed
description Growth and development of the Ceratopteris hermaphroditic gametophytes are dependent on cell proliferation in the marginal meristem, which when destroyed will regenerate at a new location on the body margin. We established a laser ablation method to destroy a single initial cell in the meristem. Ablation caused the cessation of cell proliferation accompanied by the disappearance of the expression of an auxin synthesis gene (CrTAA2) and a cell proliferation marker gene (CrWOXB). New meristem regeneration occurred within a predictable distance from the original two days post-ablation, signified by cell proliferation and the expression of CrTAA2. Treatment with the naturally occurring auxin indole-3-acetic acid (IAA), synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D), or the transport inhibitor naphthylphthalamic acid (NPA) altered positioning of the original marginal meristem toward the apex of the gametophyte. IAA altered positioning of the regenerated meristem after damaging the original meristem. A model of auxin involvement in the positioning of the marginal meristem in Ceratopteris is presented to encompass these results.
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spelling pubmed-106475182023-10-31 Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration Withers, Kelley A. Kvamme, Athena Youngstrom, Christopher E. Yarvis, Rebekah M. Orpano, Rachel Simons, Gabriel P. Irish, Erin E. Cheng, Chi-Lien Int J Mol Sci Article Growth and development of the Ceratopteris hermaphroditic gametophytes are dependent on cell proliferation in the marginal meristem, which when destroyed will regenerate at a new location on the body margin. We established a laser ablation method to destroy a single initial cell in the meristem. Ablation caused the cessation of cell proliferation accompanied by the disappearance of the expression of an auxin synthesis gene (CrTAA2) and a cell proliferation marker gene (CrWOXB). New meristem regeneration occurred within a predictable distance from the original two days post-ablation, signified by cell proliferation and the expression of CrTAA2. Treatment with the naturally occurring auxin indole-3-acetic acid (IAA), synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D), or the transport inhibitor naphthylphthalamic acid (NPA) altered positioning of the original marginal meristem toward the apex of the gametophyte. IAA altered positioning of the regenerated meristem after damaging the original meristem. A model of auxin involvement in the positioning of the marginal meristem in Ceratopteris is presented to encompass these results. MDPI 2023-10-31 /pmc/articles/PMC10647518/ /pubmed/37958815 http://dx.doi.org/10.3390/ijms242115832 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Withers, Kelley A.
Kvamme, Athena
Youngstrom, Christopher E.
Yarvis, Rebekah M.
Orpano, Rachel
Simons, Gabriel P.
Irish, Erin E.
Cheng, Chi-Lien
Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration
title Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration
title_full Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration
title_fullStr Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration
title_full_unstemmed Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration
title_short Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration
title_sort auxin involvement in ceratopteris gametophyte meristem regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10647518/
https://www.ncbi.nlm.nih.gov/pubmed/37958815
http://dx.doi.org/10.3390/ijms242115832
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