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Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC) is associated with high rates of metastasis and recurrence, and is one of the most common causes of cancer-associated death worldwide. This study examined the protein changes within circulating exosomes in patients with HCC against those in healthy people using isobari...

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Autores principales: Huang, Lien-Hung, Rau, Cheng-Shyuan, Liu, Yueh-Wei, Lin, Hui-Ping, Wu, Yi-Chan, Tsai, Chia-Wen, Chien, Peng-Chen, Wu, Chia-Jung, Huang, Chun-Ying, Hsieh, Ting-Min, Hsieh, Ching-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10647698/
https://www.ncbi.nlm.nih.gov/pubmed/37958632
http://dx.doi.org/10.3390/ijms242115652
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author Huang, Lien-Hung
Rau, Cheng-Shyuan
Liu, Yueh-Wei
Lin, Hui-Ping
Wu, Yi-Chan
Tsai, Chia-Wen
Chien, Peng-Chen
Wu, Chia-Jung
Huang, Chun-Ying
Hsieh, Ting-Min
Hsieh, Ching-Hua
author_facet Huang, Lien-Hung
Rau, Cheng-Shyuan
Liu, Yueh-Wei
Lin, Hui-Ping
Wu, Yi-Chan
Tsai, Chia-Wen
Chien, Peng-Chen
Wu, Chia-Jung
Huang, Chun-Ying
Hsieh, Ting-Min
Hsieh, Ching-Hua
author_sort Huang, Lien-Hung
collection PubMed
description Hepatocellular carcinoma (HCC) is associated with high rates of metastasis and recurrence, and is one of the most common causes of cancer-associated death worldwide. This study examined the protein changes within circulating exosomes in patients with HCC against those in healthy people using isobaric tags for a relative or absolute quantitation (iTRAQ)-based quantitative proteomics analysis. The protein levels of von Willebrand factor (VWF), cathelicidin antimicrobial peptide (CAMP), and proteasome subunit beta type-2 (PSMB2) were altered in HCC. The increased levels of VWF and PSMB2 but decreased CAMP levels in the serum of patients with HCC were validated by enzyme-linked immunosorbent assays. The level of CAMP (the only cathelicidin found in humans) also decreased in the circulating exosomes and buffy coat of the HCC patients. The serum with reduced levels of CAMP protein in the HCC patients increased the cell proliferation of Huh-7 cells; this effect was reduced following the addition of CAMP protein. The depletion of CAMP proteins in the serum of healthy people enhances the cell proliferation of Huh-7 cells. In addition, supplementation with synthetic CAMP reduces cell proliferation in a dose-dependent manner and significantly delays G1-S transition in Huh-7 cells. This implies that CAMP may act as a tumor suppressor in HCC.
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spelling pubmed-106476982023-10-27 Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma Huang, Lien-Hung Rau, Cheng-Shyuan Liu, Yueh-Wei Lin, Hui-Ping Wu, Yi-Chan Tsai, Chia-Wen Chien, Peng-Chen Wu, Chia-Jung Huang, Chun-Ying Hsieh, Ting-Min Hsieh, Ching-Hua Int J Mol Sci Article Hepatocellular carcinoma (HCC) is associated with high rates of metastasis and recurrence, and is one of the most common causes of cancer-associated death worldwide. This study examined the protein changes within circulating exosomes in patients with HCC against those in healthy people using isobaric tags for a relative or absolute quantitation (iTRAQ)-based quantitative proteomics analysis. The protein levels of von Willebrand factor (VWF), cathelicidin antimicrobial peptide (CAMP), and proteasome subunit beta type-2 (PSMB2) were altered in HCC. The increased levels of VWF and PSMB2 but decreased CAMP levels in the serum of patients with HCC were validated by enzyme-linked immunosorbent assays. The level of CAMP (the only cathelicidin found in humans) also decreased in the circulating exosomes and buffy coat of the HCC patients. The serum with reduced levels of CAMP protein in the HCC patients increased the cell proliferation of Huh-7 cells; this effect was reduced following the addition of CAMP protein. The depletion of CAMP proteins in the serum of healthy people enhances the cell proliferation of Huh-7 cells. In addition, supplementation with synthetic CAMP reduces cell proliferation in a dose-dependent manner and significantly delays G1-S transition in Huh-7 cells. This implies that CAMP may act as a tumor suppressor in HCC. MDPI 2023-10-27 /pmc/articles/PMC10647698/ /pubmed/37958632 http://dx.doi.org/10.3390/ijms242115652 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Lien-Hung
Rau, Cheng-Shyuan
Liu, Yueh-Wei
Lin, Hui-Ping
Wu, Yi-Chan
Tsai, Chia-Wen
Chien, Peng-Chen
Wu, Chia-Jung
Huang, Chun-Ying
Hsieh, Ting-Min
Hsieh, Ching-Hua
Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma
title Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma
title_full Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma
title_fullStr Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma
title_full_unstemmed Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma
title_short Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma
title_sort cathelicidin antimicrobial peptide acts as a tumor suppressor in hepatocellular carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10647698/
https://www.ncbi.nlm.nih.gov/pubmed/37958632
http://dx.doi.org/10.3390/ijms242115652
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