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Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma
Hepatocellular carcinoma (HCC) is associated with high rates of metastasis and recurrence, and is one of the most common causes of cancer-associated death worldwide. This study examined the protein changes within circulating exosomes in patients with HCC against those in healthy people using isobari...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10647698/ https://www.ncbi.nlm.nih.gov/pubmed/37958632 http://dx.doi.org/10.3390/ijms242115652 |
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author | Huang, Lien-Hung Rau, Cheng-Shyuan Liu, Yueh-Wei Lin, Hui-Ping Wu, Yi-Chan Tsai, Chia-Wen Chien, Peng-Chen Wu, Chia-Jung Huang, Chun-Ying Hsieh, Ting-Min Hsieh, Ching-Hua |
author_facet | Huang, Lien-Hung Rau, Cheng-Shyuan Liu, Yueh-Wei Lin, Hui-Ping Wu, Yi-Chan Tsai, Chia-Wen Chien, Peng-Chen Wu, Chia-Jung Huang, Chun-Ying Hsieh, Ting-Min Hsieh, Ching-Hua |
author_sort | Huang, Lien-Hung |
collection | PubMed |
description | Hepatocellular carcinoma (HCC) is associated with high rates of metastasis and recurrence, and is one of the most common causes of cancer-associated death worldwide. This study examined the protein changes within circulating exosomes in patients with HCC against those in healthy people using isobaric tags for a relative or absolute quantitation (iTRAQ)-based quantitative proteomics analysis. The protein levels of von Willebrand factor (VWF), cathelicidin antimicrobial peptide (CAMP), and proteasome subunit beta type-2 (PSMB2) were altered in HCC. The increased levels of VWF and PSMB2 but decreased CAMP levels in the serum of patients with HCC were validated by enzyme-linked immunosorbent assays. The level of CAMP (the only cathelicidin found in humans) also decreased in the circulating exosomes and buffy coat of the HCC patients. The serum with reduced levels of CAMP protein in the HCC patients increased the cell proliferation of Huh-7 cells; this effect was reduced following the addition of CAMP protein. The depletion of CAMP proteins in the serum of healthy people enhances the cell proliferation of Huh-7 cells. In addition, supplementation with synthetic CAMP reduces cell proliferation in a dose-dependent manner and significantly delays G1-S transition in Huh-7 cells. This implies that CAMP may act as a tumor suppressor in HCC. |
format | Online Article Text |
id | pubmed-10647698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106476982023-10-27 Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma Huang, Lien-Hung Rau, Cheng-Shyuan Liu, Yueh-Wei Lin, Hui-Ping Wu, Yi-Chan Tsai, Chia-Wen Chien, Peng-Chen Wu, Chia-Jung Huang, Chun-Ying Hsieh, Ting-Min Hsieh, Ching-Hua Int J Mol Sci Article Hepatocellular carcinoma (HCC) is associated with high rates of metastasis and recurrence, and is one of the most common causes of cancer-associated death worldwide. This study examined the protein changes within circulating exosomes in patients with HCC against those in healthy people using isobaric tags for a relative or absolute quantitation (iTRAQ)-based quantitative proteomics analysis. The protein levels of von Willebrand factor (VWF), cathelicidin antimicrobial peptide (CAMP), and proteasome subunit beta type-2 (PSMB2) were altered in HCC. The increased levels of VWF and PSMB2 but decreased CAMP levels in the serum of patients with HCC were validated by enzyme-linked immunosorbent assays. The level of CAMP (the only cathelicidin found in humans) also decreased in the circulating exosomes and buffy coat of the HCC patients. The serum with reduced levels of CAMP protein in the HCC patients increased the cell proliferation of Huh-7 cells; this effect was reduced following the addition of CAMP protein. The depletion of CAMP proteins in the serum of healthy people enhances the cell proliferation of Huh-7 cells. In addition, supplementation with synthetic CAMP reduces cell proliferation in a dose-dependent manner and significantly delays G1-S transition in Huh-7 cells. This implies that CAMP may act as a tumor suppressor in HCC. MDPI 2023-10-27 /pmc/articles/PMC10647698/ /pubmed/37958632 http://dx.doi.org/10.3390/ijms242115652 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Huang, Lien-Hung Rau, Cheng-Shyuan Liu, Yueh-Wei Lin, Hui-Ping Wu, Yi-Chan Tsai, Chia-Wen Chien, Peng-Chen Wu, Chia-Jung Huang, Chun-Ying Hsieh, Ting-Min Hsieh, Ching-Hua Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma |
title | Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma |
title_full | Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma |
title_fullStr | Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma |
title_full_unstemmed | Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma |
title_short | Cathelicidin Antimicrobial Peptide Acts as a Tumor Suppressor in Hepatocellular Carcinoma |
title_sort | cathelicidin antimicrobial peptide acts as a tumor suppressor in hepatocellular carcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10647698/ https://www.ncbi.nlm.nih.gov/pubmed/37958632 http://dx.doi.org/10.3390/ijms242115652 |
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