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Artemisia annua Extract Attenuate Doxorubicin-Induced Hepatic Injury via PI-3K/Akt/Nrf-2-Mediated Signaling Pathway in Rats

Doxorubicin (DOX), which is used to treat cancer, has harmful effects that limit its therapeutic application. Finding preventative agents to thwart DOX-caused injuries is thus imperative. Artemisia annua has numerous biomedical uses. This study aims to investigate the attenuative effect of Artemisia...

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Autores principales: El-Said, Karim Samy, Haidyrah, Ahmed S., Mobasher, Maysa A., Khayyat, Arwa Ishaq A., Shakoori, Afnan, Al-Sowayan, Noorah Saleh, Barnawi, Ibrahim Omar, Mariah, Reham A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10647718/
https://www.ncbi.nlm.nih.gov/pubmed/37958509
http://dx.doi.org/10.3390/ijms242115525
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author El-Said, Karim Samy
Haidyrah, Ahmed S.
Mobasher, Maysa A.
Khayyat, Arwa Ishaq A.
Shakoori, Afnan
Al-Sowayan, Noorah Saleh
Barnawi, Ibrahim Omar
Mariah, Reham A.
author_facet El-Said, Karim Samy
Haidyrah, Ahmed S.
Mobasher, Maysa A.
Khayyat, Arwa Ishaq A.
Shakoori, Afnan
Al-Sowayan, Noorah Saleh
Barnawi, Ibrahim Omar
Mariah, Reham A.
author_sort El-Said, Karim Samy
collection PubMed
description Doxorubicin (DOX), which is used to treat cancer, has harmful effects that limit its therapeutic application. Finding preventative agents to thwart DOX-caused injuries is thus imperative. Artemisia annua has numerous biomedical uses. This study aims to investigate the attenuative effect of Artemisia annua leaf extract (AALE) treatment on DOX-induced hepatic toxicity in male rats. A phytochemical screening of AALE was evaluated. Forty male rats were used; G1 was a negative control group, G2 was injected with AALE (150 mg/kg) intraperitoneally (i.p) daily for a month, 4 mg/kg of DOX was given i.p to G3 once a week for a month, and G4 was injected with DOX as G3 and with AALE as G2. Body weight changes and biochemical, molecular, and histopathological investigations were assessed. The results showed that AALE contains promising phytochemical constituents that contribute to several potential biomedical applications. AALE mitigated the hepatotoxicity induced by DOX in rats as evidenced by restoring the alterations in the biochemical parameters, antioxidant gene expression, and hepatic histopathological alterations in rats. Importantly, the impact of AALE against the hepatic deterioration resulting from DOX treatment is through activation of the PI-3K/Akt/Nrf-2 signaling, which in turn induces the antioxidant agents.
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spelling pubmed-106477182023-10-24 Artemisia annua Extract Attenuate Doxorubicin-Induced Hepatic Injury via PI-3K/Akt/Nrf-2-Mediated Signaling Pathway in Rats El-Said, Karim Samy Haidyrah, Ahmed S. Mobasher, Maysa A. Khayyat, Arwa Ishaq A. Shakoori, Afnan Al-Sowayan, Noorah Saleh Barnawi, Ibrahim Omar Mariah, Reham A. Int J Mol Sci Article Doxorubicin (DOX), which is used to treat cancer, has harmful effects that limit its therapeutic application. Finding preventative agents to thwart DOX-caused injuries is thus imperative. Artemisia annua has numerous biomedical uses. This study aims to investigate the attenuative effect of Artemisia annua leaf extract (AALE) treatment on DOX-induced hepatic toxicity in male rats. A phytochemical screening of AALE was evaluated. Forty male rats were used; G1 was a negative control group, G2 was injected with AALE (150 mg/kg) intraperitoneally (i.p) daily for a month, 4 mg/kg of DOX was given i.p to G3 once a week for a month, and G4 was injected with DOX as G3 and with AALE as G2. Body weight changes and biochemical, molecular, and histopathological investigations were assessed. The results showed that AALE contains promising phytochemical constituents that contribute to several potential biomedical applications. AALE mitigated the hepatotoxicity induced by DOX in rats as evidenced by restoring the alterations in the biochemical parameters, antioxidant gene expression, and hepatic histopathological alterations in rats. Importantly, the impact of AALE against the hepatic deterioration resulting from DOX treatment is through activation of the PI-3K/Akt/Nrf-2 signaling, which in turn induces the antioxidant agents. MDPI 2023-10-24 /pmc/articles/PMC10647718/ /pubmed/37958509 http://dx.doi.org/10.3390/ijms242115525 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
El-Said, Karim Samy
Haidyrah, Ahmed S.
Mobasher, Maysa A.
Khayyat, Arwa Ishaq A.
Shakoori, Afnan
Al-Sowayan, Noorah Saleh
Barnawi, Ibrahim Omar
Mariah, Reham A.
Artemisia annua Extract Attenuate Doxorubicin-Induced Hepatic Injury via PI-3K/Akt/Nrf-2-Mediated Signaling Pathway in Rats
title Artemisia annua Extract Attenuate Doxorubicin-Induced Hepatic Injury via PI-3K/Akt/Nrf-2-Mediated Signaling Pathway in Rats
title_full Artemisia annua Extract Attenuate Doxorubicin-Induced Hepatic Injury via PI-3K/Akt/Nrf-2-Mediated Signaling Pathway in Rats
title_fullStr Artemisia annua Extract Attenuate Doxorubicin-Induced Hepatic Injury via PI-3K/Akt/Nrf-2-Mediated Signaling Pathway in Rats
title_full_unstemmed Artemisia annua Extract Attenuate Doxorubicin-Induced Hepatic Injury via PI-3K/Akt/Nrf-2-Mediated Signaling Pathway in Rats
title_short Artemisia annua Extract Attenuate Doxorubicin-Induced Hepatic Injury via PI-3K/Akt/Nrf-2-Mediated Signaling Pathway in Rats
title_sort artemisia annua extract attenuate doxorubicin-induced hepatic injury via pi-3k/akt/nrf-2-mediated signaling pathway in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10647718/
https://www.ncbi.nlm.nih.gov/pubmed/37958509
http://dx.doi.org/10.3390/ijms242115525
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