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Label-Free SERS and MD Analysis of Biomarkers for Rapid Point-of-Care Sensors Detecting Head and Neck Cancer and Infections

For the progress of point-of-care medicine, where individual health status can be easily and quickly monitored using a handheld sensor, saliva serves as one of the best-suited body fluids thanks to its availability and abundance of physiological indicators. Salivary biomarkers, combined with rapid a...

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Autores principales: Farnesi, Edoardo, Rinaldi, Silvia, Liu, Chen, Ballmaier, Jonas, Guntinas-Lichius, Orlando, Schmitt, Michael, Cialla-May, Dana, Popp, Juergen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648186/
https://www.ncbi.nlm.nih.gov/pubmed/37960614
http://dx.doi.org/10.3390/s23218915
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author Farnesi, Edoardo
Rinaldi, Silvia
Liu, Chen
Ballmaier, Jonas
Guntinas-Lichius, Orlando
Schmitt, Michael
Cialla-May, Dana
Popp, Juergen
author_facet Farnesi, Edoardo
Rinaldi, Silvia
Liu, Chen
Ballmaier, Jonas
Guntinas-Lichius, Orlando
Schmitt, Michael
Cialla-May, Dana
Popp, Juergen
author_sort Farnesi, Edoardo
collection PubMed
description For the progress of point-of-care medicine, where individual health status can be easily and quickly monitored using a handheld sensor, saliva serves as one of the best-suited body fluids thanks to its availability and abundance of physiological indicators. Salivary biomarkers, combined with rapid and highly sensitive detection tools, may pave the way to new real-time health monitoring and personalized preventative therapy branches using saliva as a target matrix. Saliva is increasing in importance in liquid biopsy, a non-invasive approach that helps physicians diagnose and characterize specific diseases in patients. Here, we propose a proof-of-concept study combining the unique specificity in biomolecular recognition provided by surface-enhanced Raman spectroscopy (SERS) in combination with molecular dynamics (MD) simulations, which give leave to explore the biomolecular absorption mechanism on nanoparticle surfaces, in order to verify the traceability of two validated salivary indicators, i.e., interleukin-8 (IL-8) and lysozyme (LYZ), implicated in oropharyngeal squamous cell carcinoma (OSCC) and oral infection. This strategy simultaneously assures the detection and interpretation of protein biomarkers in saliva, ultimately opening a new route for the evolution of fast and accurate point-of-care SERS-based sensors of interest in precision medicine diagnostics.
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spelling pubmed-106481862023-11-02 Label-Free SERS and MD Analysis of Biomarkers for Rapid Point-of-Care Sensors Detecting Head and Neck Cancer and Infections Farnesi, Edoardo Rinaldi, Silvia Liu, Chen Ballmaier, Jonas Guntinas-Lichius, Orlando Schmitt, Michael Cialla-May, Dana Popp, Juergen Sensors (Basel) Article For the progress of point-of-care medicine, where individual health status can be easily and quickly monitored using a handheld sensor, saliva serves as one of the best-suited body fluids thanks to its availability and abundance of physiological indicators. Salivary biomarkers, combined with rapid and highly sensitive detection tools, may pave the way to new real-time health monitoring and personalized preventative therapy branches using saliva as a target matrix. Saliva is increasing in importance in liquid biopsy, a non-invasive approach that helps physicians diagnose and characterize specific diseases in patients. Here, we propose a proof-of-concept study combining the unique specificity in biomolecular recognition provided by surface-enhanced Raman spectroscopy (SERS) in combination with molecular dynamics (MD) simulations, which give leave to explore the biomolecular absorption mechanism on nanoparticle surfaces, in order to verify the traceability of two validated salivary indicators, i.e., interleukin-8 (IL-8) and lysozyme (LYZ), implicated in oropharyngeal squamous cell carcinoma (OSCC) and oral infection. This strategy simultaneously assures the detection and interpretation of protein biomarkers in saliva, ultimately opening a new route for the evolution of fast and accurate point-of-care SERS-based sensors of interest in precision medicine diagnostics. MDPI 2023-11-02 /pmc/articles/PMC10648186/ /pubmed/37960614 http://dx.doi.org/10.3390/s23218915 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Farnesi, Edoardo
Rinaldi, Silvia
Liu, Chen
Ballmaier, Jonas
Guntinas-Lichius, Orlando
Schmitt, Michael
Cialla-May, Dana
Popp, Juergen
Label-Free SERS and MD Analysis of Biomarkers for Rapid Point-of-Care Sensors Detecting Head and Neck Cancer and Infections
title Label-Free SERS and MD Analysis of Biomarkers for Rapid Point-of-Care Sensors Detecting Head and Neck Cancer and Infections
title_full Label-Free SERS and MD Analysis of Biomarkers for Rapid Point-of-Care Sensors Detecting Head and Neck Cancer and Infections
title_fullStr Label-Free SERS and MD Analysis of Biomarkers for Rapid Point-of-Care Sensors Detecting Head and Neck Cancer and Infections
title_full_unstemmed Label-Free SERS and MD Analysis of Biomarkers for Rapid Point-of-Care Sensors Detecting Head and Neck Cancer and Infections
title_short Label-Free SERS and MD Analysis of Biomarkers for Rapid Point-of-Care Sensors Detecting Head and Neck Cancer and Infections
title_sort label-free sers and md analysis of biomarkers for rapid point-of-care sensors detecting head and neck cancer and infections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648186/
https://www.ncbi.nlm.nih.gov/pubmed/37960614
http://dx.doi.org/10.3390/s23218915
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