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Vancomycin-Loaded 3D-Printed Polylactic Acid–Hydroxyapatite Scaffolds for Bone Tissue Engineering
The regeneration of bone remains one of the main challenges in the biomedical field, with the need to provide more personalized and multifunctional solutions. The other persistent challenge is related to the local prevention of infections after implantation surgery. To fulfill the first one and prov...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648244/ https://www.ncbi.nlm.nih.gov/pubmed/37959930 http://dx.doi.org/10.3390/polym15214250 |
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author | Pérez-Davila, Sara Potel-Alvarellos, Carmen Carballo, Raquel González-Rodríguez, Laura López-Álvarez, Miriam Serra, Julia Díaz-Rodríguez, Patricia Landín, Mariana González, Pío |
author_facet | Pérez-Davila, Sara Potel-Alvarellos, Carmen Carballo, Raquel González-Rodríguez, Laura López-Álvarez, Miriam Serra, Julia Díaz-Rodríguez, Patricia Landín, Mariana González, Pío |
author_sort | Pérez-Davila, Sara |
collection | PubMed |
description | The regeneration of bone remains one of the main challenges in the biomedical field, with the need to provide more personalized and multifunctional solutions. The other persistent challenge is related to the local prevention of infections after implantation surgery. To fulfill the first one and provide customized scaffolds with complex geometries, 3D printing is being investigated, with polylactic acid (PLA) as the biomaterial mostly used, given its thermoplastic properties. The 3D printing of PLA in combination with hydroxyapatite (HA) is also under research, to mimic the native mechanical and biological properties, providing more functional scaffolds. Finally, to fulfill the second one, antibacterial drugs locally incorporated into biodegradable scaffolds are also under investigation. This work aims to develop vancomycin-loaded 3D-printed PLA–HA scaffolds offering a dual functionality: local prevention of infections and personalized biodegradable scaffolds with osseointegrative properties. For this, the antibacterial drug vancomycin was incorporated into 3D-printed PLA–HA scaffolds using three loading methodologies: (1) dip coating, (2) drop coating, and (3) direct incorporation in the 3D printing with PLA and HA. A systematic characterization was performed, including release kinetics, Staphylococcus aureus antibacterial/antibiofilm activities and cytocompatibility. The results demonstrated the feasibility of the vancomycin-loaded 3D-printed PLA–HA scaffolds as drug-releasing vehicles with significant antibacterial effects for the three methodologies. In relation to the drug release kinetics, the (1) dip- and (2) drop-coating methodologies achieved burst release (first 60 min) of around 80–90% of the loaded vancomycin, followed by a slower release of the remaining drug for up to 48 h, while the (3) 3D printing presented an extended release beyond 7 days as the polymer degraded. The cytocompatibility of the vancomycin-loaded scaffolds was also confirmed. |
format | Online Article Text |
id | pubmed-10648244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106482442023-10-28 Vancomycin-Loaded 3D-Printed Polylactic Acid–Hydroxyapatite Scaffolds for Bone Tissue Engineering Pérez-Davila, Sara Potel-Alvarellos, Carmen Carballo, Raquel González-Rodríguez, Laura López-Álvarez, Miriam Serra, Julia Díaz-Rodríguez, Patricia Landín, Mariana González, Pío Polymers (Basel) Article The regeneration of bone remains one of the main challenges in the biomedical field, with the need to provide more personalized and multifunctional solutions. The other persistent challenge is related to the local prevention of infections after implantation surgery. To fulfill the first one and provide customized scaffolds with complex geometries, 3D printing is being investigated, with polylactic acid (PLA) as the biomaterial mostly used, given its thermoplastic properties. The 3D printing of PLA in combination with hydroxyapatite (HA) is also under research, to mimic the native mechanical and biological properties, providing more functional scaffolds. Finally, to fulfill the second one, antibacterial drugs locally incorporated into biodegradable scaffolds are also under investigation. This work aims to develop vancomycin-loaded 3D-printed PLA–HA scaffolds offering a dual functionality: local prevention of infections and personalized biodegradable scaffolds with osseointegrative properties. For this, the antibacterial drug vancomycin was incorporated into 3D-printed PLA–HA scaffolds using three loading methodologies: (1) dip coating, (2) drop coating, and (3) direct incorporation in the 3D printing with PLA and HA. A systematic characterization was performed, including release kinetics, Staphylococcus aureus antibacterial/antibiofilm activities and cytocompatibility. The results demonstrated the feasibility of the vancomycin-loaded 3D-printed PLA–HA scaffolds as drug-releasing vehicles with significant antibacterial effects for the three methodologies. In relation to the drug release kinetics, the (1) dip- and (2) drop-coating methodologies achieved burst release (first 60 min) of around 80–90% of the loaded vancomycin, followed by a slower release of the remaining drug for up to 48 h, while the (3) 3D printing presented an extended release beyond 7 days as the polymer degraded. The cytocompatibility of the vancomycin-loaded scaffolds was also confirmed. MDPI 2023-10-28 /pmc/articles/PMC10648244/ /pubmed/37959930 http://dx.doi.org/10.3390/polym15214250 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pérez-Davila, Sara Potel-Alvarellos, Carmen Carballo, Raquel González-Rodríguez, Laura López-Álvarez, Miriam Serra, Julia Díaz-Rodríguez, Patricia Landín, Mariana González, Pío Vancomycin-Loaded 3D-Printed Polylactic Acid–Hydroxyapatite Scaffolds for Bone Tissue Engineering |
title | Vancomycin-Loaded 3D-Printed Polylactic Acid–Hydroxyapatite Scaffolds for Bone Tissue Engineering |
title_full | Vancomycin-Loaded 3D-Printed Polylactic Acid–Hydroxyapatite Scaffolds for Bone Tissue Engineering |
title_fullStr | Vancomycin-Loaded 3D-Printed Polylactic Acid–Hydroxyapatite Scaffolds for Bone Tissue Engineering |
title_full_unstemmed | Vancomycin-Loaded 3D-Printed Polylactic Acid–Hydroxyapatite Scaffolds for Bone Tissue Engineering |
title_short | Vancomycin-Loaded 3D-Printed Polylactic Acid–Hydroxyapatite Scaffolds for Bone Tissue Engineering |
title_sort | vancomycin-loaded 3d-printed polylactic acid–hydroxyapatite scaffolds for bone tissue engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648244/ https://www.ncbi.nlm.nih.gov/pubmed/37959930 http://dx.doi.org/10.3390/polym15214250 |
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