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Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid

Citrus fruits are a natural source of ascorbic acid, and exosome-like nanovesicles obtained from these fruits contain measurable levels of ascorbic acid. We tested the ability of grapefruit-derived extracellular vesicles (EVs) to inhibit the growth of human leukemic cells and leukemic patient-derive...

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Autores principales: Castelli, Germana, Logozzi, Mariantonia, Mizzoni, Davide, Di Raimo, Rossella, Cerio, Annamaria, Dolo, Vincenza, Pasquini, Luca, Screnci, Maria, Ottone, Tiziana, Testa, Ugo, Fais, Stefano, Pelosi, Elvira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648274/
https://www.ncbi.nlm.nih.gov/pubmed/37958646
http://dx.doi.org/10.3390/ijms242115663
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author Castelli, Germana
Logozzi, Mariantonia
Mizzoni, Davide
Di Raimo, Rossella
Cerio, Annamaria
Dolo, Vincenza
Pasquini, Luca
Screnci, Maria
Ottone, Tiziana
Testa, Ugo
Fais, Stefano
Pelosi, Elvira
author_facet Castelli, Germana
Logozzi, Mariantonia
Mizzoni, Davide
Di Raimo, Rossella
Cerio, Annamaria
Dolo, Vincenza
Pasquini, Luca
Screnci, Maria
Ottone, Tiziana
Testa, Ugo
Fais, Stefano
Pelosi, Elvira
author_sort Castelli, Germana
collection PubMed
description Citrus fruits are a natural source of ascorbic acid, and exosome-like nanovesicles obtained from these fruits contain measurable levels of ascorbic acid. We tested the ability of grapefruit-derived extracellular vesicles (EVs) to inhibit the growth of human leukemic cells and leukemic patient-derived bone marrow blasts. Transmission electron microscopy and nanoparticle tracking analysis (NTA) showed that the obtained EVs were homogeneous exosomes, defined as exosome-like plant-derived nanovesicles (ELPDNVs). The analysis of their content has shown measurable amounts of several molecules with potent antioxidant activity. ELPDNVs showed a time-dependent antiproliferative effect in both U937 and K562 leukemic cell lines, comparable with the effect of high-dosage ascorbic acid (2 mM). This result was confirmed by a clear decrease in the number of AML blasts induced by ELPDNVs, which did not affect the number of normal cells. ELPDNVs increased the ROS levels in both AML blast cells and U937 without affecting ROS storage in normal cells, and this effect was comparable to ascorbic acid (2 mM). With our study, we propose ELPDNVs from grapefruits as a combination/supporting therapy for human leukemias with the aim to improve the effectiveness of the current therapies.
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spelling pubmed-106482742023-10-27 Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid Castelli, Germana Logozzi, Mariantonia Mizzoni, Davide Di Raimo, Rossella Cerio, Annamaria Dolo, Vincenza Pasquini, Luca Screnci, Maria Ottone, Tiziana Testa, Ugo Fais, Stefano Pelosi, Elvira Int J Mol Sci Article Citrus fruits are a natural source of ascorbic acid, and exosome-like nanovesicles obtained from these fruits contain measurable levels of ascorbic acid. We tested the ability of grapefruit-derived extracellular vesicles (EVs) to inhibit the growth of human leukemic cells and leukemic patient-derived bone marrow blasts. Transmission electron microscopy and nanoparticle tracking analysis (NTA) showed that the obtained EVs were homogeneous exosomes, defined as exosome-like plant-derived nanovesicles (ELPDNVs). The analysis of their content has shown measurable amounts of several molecules with potent antioxidant activity. ELPDNVs showed a time-dependent antiproliferative effect in both U937 and K562 leukemic cell lines, comparable with the effect of high-dosage ascorbic acid (2 mM). This result was confirmed by a clear decrease in the number of AML blasts induced by ELPDNVs, which did not affect the number of normal cells. ELPDNVs increased the ROS levels in both AML blast cells and U937 without affecting ROS storage in normal cells, and this effect was comparable to ascorbic acid (2 mM). With our study, we propose ELPDNVs from grapefruits as a combination/supporting therapy for human leukemias with the aim to improve the effectiveness of the current therapies. MDPI 2023-10-27 /pmc/articles/PMC10648274/ /pubmed/37958646 http://dx.doi.org/10.3390/ijms242115663 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Castelli, Germana
Logozzi, Mariantonia
Mizzoni, Davide
Di Raimo, Rossella
Cerio, Annamaria
Dolo, Vincenza
Pasquini, Luca
Screnci, Maria
Ottone, Tiziana
Testa, Ugo
Fais, Stefano
Pelosi, Elvira
Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid
title Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid
title_full Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid
title_fullStr Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid
title_full_unstemmed Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid
title_short Ex Vivo Anti-Leukemic Effect of Exosome-like Grapefruit-Derived Nanovesicles from Organic Farming—The Potential Role of Ascorbic Acid
title_sort ex vivo anti-leukemic effect of exosome-like grapefruit-derived nanovesicles from organic farming—the potential role of ascorbic acid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648274/
https://www.ncbi.nlm.nih.gov/pubmed/37958646
http://dx.doi.org/10.3390/ijms242115663
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