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Transcriptome and Physiological Analysis of Rapeseed Tolerance to Post-Flowering Temperature Increase
Climate-change-induced temperature fluctuations pose a significant threat to crop production, particularly in the Southern Hemisphere. This study investigates the transcriptome and physiological responses of rapeseed to post-flowering temperature increases, providing valuable insights into the molec...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648292/ https://www.ncbi.nlm.nih.gov/pubmed/37958577 http://dx.doi.org/10.3390/ijms242115593 |
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author | Canales, Javier Verdejo, José F. Calderini, Daniel F. |
author_facet | Canales, Javier Verdejo, José F. Calderini, Daniel F. |
author_sort | Canales, Javier |
collection | PubMed |
description | Climate-change-induced temperature fluctuations pose a significant threat to crop production, particularly in the Southern Hemisphere. This study investigates the transcriptome and physiological responses of rapeseed to post-flowering temperature increases, providing valuable insights into the molecular mechanisms underlying rapeseed tolerance to heat stress. Two rapeseed genotypes, Lumen and Solar, were assessed under control and heat stress conditions in field experiments conducted in Valdivia, Chile. Results showed that seed yield and seed number were negatively affected by heat stress, with genotype-specific responses. Lumen exhibited an average of 9.3% seed yield reduction, whereas Solar showed a 28.7% reduction. RNA-seq analysis of siliques and seeds revealed tissue-specific responses to heat stress, with siliques being more sensitive to temperature stress. Hierarchical clustering analysis identified distinct gene clusters reflecting different aspects of heat stress adaptation in siliques, with a role for protein folding in maintaining silique development and seed quality under high-temperature conditions. In seeds, three distinct patterns of heat-responsive gene expression were observed, with genes involved in protein folding and response to heat showing genotype-specific expression. Gene coexpression network analysis revealed major modules for rapeseed yield and quality, as well as the trade-off between seed number and seed weight. Overall, this study contributes to understanding the molecular mechanisms underlying rapeseed tolerance to heat stress and can inform crop improvement strategies targeting yield optimization under changing environmental conditions. |
format | Online Article Text |
id | pubmed-10648292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106482922023-10-26 Transcriptome and Physiological Analysis of Rapeseed Tolerance to Post-Flowering Temperature Increase Canales, Javier Verdejo, José F. Calderini, Daniel F. Int J Mol Sci Article Climate-change-induced temperature fluctuations pose a significant threat to crop production, particularly in the Southern Hemisphere. This study investigates the transcriptome and physiological responses of rapeseed to post-flowering temperature increases, providing valuable insights into the molecular mechanisms underlying rapeseed tolerance to heat stress. Two rapeseed genotypes, Lumen and Solar, were assessed under control and heat stress conditions in field experiments conducted in Valdivia, Chile. Results showed that seed yield and seed number were negatively affected by heat stress, with genotype-specific responses. Lumen exhibited an average of 9.3% seed yield reduction, whereas Solar showed a 28.7% reduction. RNA-seq analysis of siliques and seeds revealed tissue-specific responses to heat stress, with siliques being more sensitive to temperature stress. Hierarchical clustering analysis identified distinct gene clusters reflecting different aspects of heat stress adaptation in siliques, with a role for protein folding in maintaining silique development and seed quality under high-temperature conditions. In seeds, three distinct patterns of heat-responsive gene expression were observed, with genes involved in protein folding and response to heat showing genotype-specific expression. Gene coexpression network analysis revealed major modules for rapeseed yield and quality, as well as the trade-off between seed number and seed weight. Overall, this study contributes to understanding the molecular mechanisms underlying rapeseed tolerance to heat stress and can inform crop improvement strategies targeting yield optimization under changing environmental conditions. MDPI 2023-10-26 /pmc/articles/PMC10648292/ /pubmed/37958577 http://dx.doi.org/10.3390/ijms242115593 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Canales, Javier Verdejo, José F. Calderini, Daniel F. Transcriptome and Physiological Analysis of Rapeseed Tolerance to Post-Flowering Temperature Increase |
title | Transcriptome and Physiological Analysis of Rapeseed Tolerance to Post-Flowering Temperature Increase |
title_full | Transcriptome and Physiological Analysis of Rapeseed Tolerance to Post-Flowering Temperature Increase |
title_fullStr | Transcriptome and Physiological Analysis of Rapeseed Tolerance to Post-Flowering Temperature Increase |
title_full_unstemmed | Transcriptome and Physiological Analysis of Rapeseed Tolerance to Post-Flowering Temperature Increase |
title_short | Transcriptome and Physiological Analysis of Rapeseed Tolerance to Post-Flowering Temperature Increase |
title_sort | transcriptome and physiological analysis of rapeseed tolerance to post-flowering temperature increase |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648292/ https://www.ncbi.nlm.nih.gov/pubmed/37958577 http://dx.doi.org/10.3390/ijms242115593 |
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