Cargando…

Transcriptome-Wide Identification of TCP Transcription Factor Family Members in Pinus massoniana and Their Expression in Regulation of Development and in Response to Stress

Pinus massoniana is an important coniferous tree species for barren mountain afforestation with enormous ecological and economic significance. It has strong adaptability to the environment. TEOSINTE BRANCHED 1/CYCLOIDEA/PCF (TCP) transcription factors (TFs) play crucial roles in plant stress respons...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Mengyang, Agassin, Romaric Hippolyte, Huang, Zichen, Wang, Dengbao, Yao, Sheng, Ji, Kongshu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648340/
https://www.ncbi.nlm.nih.gov/pubmed/37958919
http://dx.doi.org/10.3390/ijms242115938
_version_ 1785135316245413888
author Zhang, Mengyang
Agassin, Romaric Hippolyte
Huang, Zichen
Wang, Dengbao
Yao, Sheng
Ji, Kongshu
author_facet Zhang, Mengyang
Agassin, Romaric Hippolyte
Huang, Zichen
Wang, Dengbao
Yao, Sheng
Ji, Kongshu
author_sort Zhang, Mengyang
collection PubMed
description Pinus massoniana is an important coniferous tree species for barren mountain afforestation with enormous ecological and economic significance. It has strong adaptability to the environment. TEOSINTE BRANCHED 1/CYCLOIDEA/PCF (TCP) transcription factors (TFs) play crucial roles in plant stress response, hormone signal transduction, and development processes. At present, TCP TFs have been widely studied in multiple plant species, but research in P. massoniana has not been carried out. In this study, 13 PmTCP TFs were identified from the transcriptomes of P. massoniana. The phylogenetic results revealed that these PmTCP members were divided into two categories: Class I and Class II. Each PmTCP TF contained a conserved TCP domain, and the conserved motif types and numbers were similar in the same subgroup. According to the transcriptional profiling analysis under drought stress conditions, it was found that seven PmTCP genes responded to drought treatment to varying degrees. The qRT-PCR results showed that the majority of PmTCP genes were significantly expressed in the needles and may play a role in the developmental stage. Meanwhile, the PmTCPs could respond to several stresses and hormone treatments at different levels, which may be important for stress resistance. In addition, PmTCP7 and PmTCP12 were nuclear localization proteins, and PmTCP7 was a transcriptional suppressor. These results will help to explore the regulatory factors related to the growth and development of P. massoniana, enhance its stress resistance, and lay the foundation for further exploration of the physiological effects on PmTCPs.
format Online
Article
Text
id pubmed-10648340
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106483402023-11-03 Transcriptome-Wide Identification of TCP Transcription Factor Family Members in Pinus massoniana and Their Expression in Regulation of Development and in Response to Stress Zhang, Mengyang Agassin, Romaric Hippolyte Huang, Zichen Wang, Dengbao Yao, Sheng Ji, Kongshu Int J Mol Sci Article Pinus massoniana is an important coniferous tree species for barren mountain afforestation with enormous ecological and economic significance. It has strong adaptability to the environment. TEOSINTE BRANCHED 1/CYCLOIDEA/PCF (TCP) transcription factors (TFs) play crucial roles in plant stress response, hormone signal transduction, and development processes. At present, TCP TFs have been widely studied in multiple plant species, but research in P. massoniana has not been carried out. In this study, 13 PmTCP TFs were identified from the transcriptomes of P. massoniana. The phylogenetic results revealed that these PmTCP members were divided into two categories: Class I and Class II. Each PmTCP TF contained a conserved TCP domain, and the conserved motif types and numbers were similar in the same subgroup. According to the transcriptional profiling analysis under drought stress conditions, it was found that seven PmTCP genes responded to drought treatment to varying degrees. The qRT-PCR results showed that the majority of PmTCP genes were significantly expressed in the needles and may play a role in the developmental stage. Meanwhile, the PmTCPs could respond to several stresses and hormone treatments at different levels, which may be important for stress resistance. In addition, PmTCP7 and PmTCP12 were nuclear localization proteins, and PmTCP7 was a transcriptional suppressor. These results will help to explore the regulatory factors related to the growth and development of P. massoniana, enhance its stress resistance, and lay the foundation for further exploration of the physiological effects on PmTCPs. MDPI 2023-11-03 /pmc/articles/PMC10648340/ /pubmed/37958919 http://dx.doi.org/10.3390/ijms242115938 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Mengyang
Agassin, Romaric Hippolyte
Huang, Zichen
Wang, Dengbao
Yao, Sheng
Ji, Kongshu
Transcriptome-Wide Identification of TCP Transcription Factor Family Members in Pinus massoniana and Their Expression in Regulation of Development and in Response to Stress
title Transcriptome-Wide Identification of TCP Transcription Factor Family Members in Pinus massoniana and Their Expression in Regulation of Development and in Response to Stress
title_full Transcriptome-Wide Identification of TCP Transcription Factor Family Members in Pinus massoniana and Their Expression in Regulation of Development and in Response to Stress
title_fullStr Transcriptome-Wide Identification of TCP Transcription Factor Family Members in Pinus massoniana and Their Expression in Regulation of Development and in Response to Stress
title_full_unstemmed Transcriptome-Wide Identification of TCP Transcription Factor Family Members in Pinus massoniana and Their Expression in Regulation of Development and in Response to Stress
title_short Transcriptome-Wide Identification of TCP Transcription Factor Family Members in Pinus massoniana and Their Expression in Regulation of Development and in Response to Stress
title_sort transcriptome-wide identification of tcp transcription factor family members in pinus massoniana and their expression in regulation of development and in response to stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648340/
https://www.ncbi.nlm.nih.gov/pubmed/37958919
http://dx.doi.org/10.3390/ijms242115938
work_keys_str_mv AT zhangmengyang transcriptomewideidentificationoftcptranscriptionfactorfamilymembersinpinusmassonianaandtheirexpressioninregulationofdevelopmentandinresponsetostress
AT agassinromarichippolyte transcriptomewideidentificationoftcptranscriptionfactorfamilymembersinpinusmassonianaandtheirexpressioninregulationofdevelopmentandinresponsetostress
AT huangzichen transcriptomewideidentificationoftcptranscriptionfactorfamilymembersinpinusmassonianaandtheirexpressioninregulationofdevelopmentandinresponsetostress
AT wangdengbao transcriptomewideidentificationoftcptranscriptionfactorfamilymembersinpinusmassonianaandtheirexpressioninregulationofdevelopmentandinresponsetostress
AT yaosheng transcriptomewideidentificationoftcptranscriptionfactorfamilymembersinpinusmassonianaandtheirexpressioninregulationofdevelopmentandinresponsetostress
AT jikongshu transcriptomewideidentificationoftcptranscriptionfactorfamilymembersinpinusmassonianaandtheirexpressioninregulationofdevelopmentandinresponsetostress