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Unlocking the Potential of Carbon Quantum Dots for Cell Imaging, Intracellular Localization, and Gene Expression Control in Arabidopsis thaliana (L.) Heynh.

Utilizing carbon quantum dots (CQDs) as biomaterials for delivering small substances has gained significant attention in recent research. However, the interactions and mechanisms of action of CQDs on plants have received relatively little focus. Herein, we investigated the transportation of CQDs int...

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Detalles Bibliográficos
Autores principales: Lin, Zhimin, Ali, Muhammad Moaaz, Yi, Xiaoyan, Zhang, Lijuan, Wang, Shaojuan, Chen, Faxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648342/
https://www.ncbi.nlm.nih.gov/pubmed/37958684
http://dx.doi.org/10.3390/ijms242115700
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author Lin, Zhimin
Ali, Muhammad Moaaz
Yi, Xiaoyan
Zhang, Lijuan
Wang, Shaojuan
Chen, Faxing
author_facet Lin, Zhimin
Ali, Muhammad Moaaz
Yi, Xiaoyan
Zhang, Lijuan
Wang, Shaojuan
Chen, Faxing
author_sort Lin, Zhimin
collection PubMed
description Utilizing carbon quantum dots (CQDs) as biomaterials for delivering small substances has gained significant attention in recent research. However, the interactions and mechanisms of action of CQDs on plants have received relatively little focus. Herein, we investigated the transportation of CQDs into various organs of Arabidopsis thaliana (L.) Heynh. via the vessel system, leading to the epigenetic inheritance of Argonaute family genes. Our findings reveal that CQDs may interact with microRNAs (miRNAs), leading to the repression of post-transcriptional regulation of target genes in the cytoplasm. Transcriptome and quantitative PCR analyses demonstrated consistent gene expression levels in offspring. Moreover, microscopic observations illustrated rapid CQD localization on cell membranes and nuclei, with increased nuclear entry at higher concentrations. Notably, our study identified an alternative regulatory microRNA, microRNA172D, for the Argonaute family genes through methylation analysis, shedding light on the connection between CQDs and microRNAs.
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spelling pubmed-106483422023-10-28 Unlocking the Potential of Carbon Quantum Dots for Cell Imaging, Intracellular Localization, and Gene Expression Control in Arabidopsis thaliana (L.) Heynh. Lin, Zhimin Ali, Muhammad Moaaz Yi, Xiaoyan Zhang, Lijuan Wang, Shaojuan Chen, Faxing Int J Mol Sci Article Utilizing carbon quantum dots (CQDs) as biomaterials for delivering small substances has gained significant attention in recent research. However, the interactions and mechanisms of action of CQDs on plants have received relatively little focus. Herein, we investigated the transportation of CQDs into various organs of Arabidopsis thaliana (L.) Heynh. via the vessel system, leading to the epigenetic inheritance of Argonaute family genes. Our findings reveal that CQDs may interact with microRNAs (miRNAs), leading to the repression of post-transcriptional regulation of target genes in the cytoplasm. Transcriptome and quantitative PCR analyses demonstrated consistent gene expression levels in offspring. Moreover, microscopic observations illustrated rapid CQD localization on cell membranes and nuclei, with increased nuclear entry at higher concentrations. Notably, our study identified an alternative regulatory microRNA, microRNA172D, for the Argonaute family genes through methylation analysis, shedding light on the connection between CQDs and microRNAs. MDPI 2023-10-28 /pmc/articles/PMC10648342/ /pubmed/37958684 http://dx.doi.org/10.3390/ijms242115700 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lin, Zhimin
Ali, Muhammad Moaaz
Yi, Xiaoyan
Zhang, Lijuan
Wang, Shaojuan
Chen, Faxing
Unlocking the Potential of Carbon Quantum Dots for Cell Imaging, Intracellular Localization, and Gene Expression Control in Arabidopsis thaliana (L.) Heynh.
title Unlocking the Potential of Carbon Quantum Dots for Cell Imaging, Intracellular Localization, and Gene Expression Control in Arabidopsis thaliana (L.) Heynh.
title_full Unlocking the Potential of Carbon Quantum Dots for Cell Imaging, Intracellular Localization, and Gene Expression Control in Arabidopsis thaliana (L.) Heynh.
title_fullStr Unlocking the Potential of Carbon Quantum Dots for Cell Imaging, Intracellular Localization, and Gene Expression Control in Arabidopsis thaliana (L.) Heynh.
title_full_unstemmed Unlocking the Potential of Carbon Quantum Dots for Cell Imaging, Intracellular Localization, and Gene Expression Control in Arabidopsis thaliana (L.) Heynh.
title_short Unlocking the Potential of Carbon Quantum Dots for Cell Imaging, Intracellular Localization, and Gene Expression Control in Arabidopsis thaliana (L.) Heynh.
title_sort unlocking the potential of carbon quantum dots for cell imaging, intracellular localization, and gene expression control in arabidopsis thaliana (l.) heynh.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648342/
https://www.ncbi.nlm.nih.gov/pubmed/37958684
http://dx.doi.org/10.3390/ijms242115700
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