Cargando…

Three-Dimensional Printing of Graphene Oxide/Poly-L-Lactic Acid Scaffolds Using Fischer–Koch Modeling

Accurately printing customizable scaffolds is a challenging task because of the complexity of bone tissue composition, organization, and mechanical behavior. Graphene oxide (GO) and poly-L-lactic acid (PLLA) have drawn attention in the field of bone regeneration. However, as far as we know, the Fisc...

Descripción completa

Detalles Bibliográficos
Autores principales: da Silva, Thamires Santos, Horvath-Pereira, Bianca de Oliveira, da Silva-Júnior, Leandro Norberto, Tenório Fireman, João Víctor Barbosa, Mattar, Michel, Félix, Marcílio, Buchaim, Rogerio Leone, Carreira, Ana Claudia Oliveira, Miglino, Maria Angelica, Soares, Marcelo Melo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648465/
https://www.ncbi.nlm.nih.gov/pubmed/37959893
http://dx.doi.org/10.3390/polym15214213
_version_ 1785135347074596864
author da Silva, Thamires Santos
Horvath-Pereira, Bianca de Oliveira
da Silva-Júnior, Leandro Norberto
Tenório Fireman, João Víctor Barbosa
Mattar, Michel
Félix, Marcílio
Buchaim, Rogerio Leone
Carreira, Ana Claudia Oliveira
Miglino, Maria Angelica
Soares, Marcelo Melo
author_facet da Silva, Thamires Santos
Horvath-Pereira, Bianca de Oliveira
da Silva-Júnior, Leandro Norberto
Tenório Fireman, João Víctor Barbosa
Mattar, Michel
Félix, Marcílio
Buchaim, Rogerio Leone
Carreira, Ana Claudia Oliveira
Miglino, Maria Angelica
Soares, Marcelo Melo
author_sort da Silva, Thamires Santos
collection PubMed
description Accurately printing customizable scaffolds is a challenging task because of the complexity of bone tissue composition, organization, and mechanical behavior. Graphene oxide (GO) and poly-L-lactic acid (PLLA) have drawn attention in the field of bone regeneration. However, as far as we know, the Fischer–Koch model of the GO/PLLA association for three-dimensional (3D) printing was not previously reported. This study characterizes the properties of GO/PLLA-printed scaffolds in order to achieve reproducibility of the trabecula, from virtual planning to the printed piece, as well as its response to a cell viability assay. Fourier-transform infrared and Raman spectroscopy were performed to evaluate the physicochemical properties of the nanocomposites. Cellular adhesion, proliferation, and growth on the nanocomposites were evaluated using scanning electron microscopy. Cell viability tests revealed no significant differences among different trabeculae and cell types, indicating that these nanocomposites were not cytotoxic. The Fischer Koch modeling yielded satisfactory results and can thus be used in studies directed at diverse medical applications, including bone tissue engineering and implants.
format Online
Article
Text
id pubmed-10648465
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106484652023-10-25 Three-Dimensional Printing of Graphene Oxide/Poly-L-Lactic Acid Scaffolds Using Fischer–Koch Modeling da Silva, Thamires Santos Horvath-Pereira, Bianca de Oliveira da Silva-Júnior, Leandro Norberto Tenório Fireman, João Víctor Barbosa Mattar, Michel Félix, Marcílio Buchaim, Rogerio Leone Carreira, Ana Claudia Oliveira Miglino, Maria Angelica Soares, Marcelo Melo Polymers (Basel) Article Accurately printing customizable scaffolds is a challenging task because of the complexity of bone tissue composition, organization, and mechanical behavior. Graphene oxide (GO) and poly-L-lactic acid (PLLA) have drawn attention in the field of bone regeneration. However, as far as we know, the Fischer–Koch model of the GO/PLLA association for three-dimensional (3D) printing was not previously reported. This study characterizes the properties of GO/PLLA-printed scaffolds in order to achieve reproducibility of the trabecula, from virtual planning to the printed piece, as well as its response to a cell viability assay. Fourier-transform infrared and Raman spectroscopy were performed to evaluate the physicochemical properties of the nanocomposites. Cellular adhesion, proliferation, and growth on the nanocomposites were evaluated using scanning electron microscopy. Cell viability tests revealed no significant differences among different trabeculae and cell types, indicating that these nanocomposites were not cytotoxic. The Fischer Koch modeling yielded satisfactory results and can thus be used in studies directed at diverse medical applications, including bone tissue engineering and implants. MDPI 2023-10-25 /pmc/articles/PMC10648465/ /pubmed/37959893 http://dx.doi.org/10.3390/polym15214213 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
da Silva, Thamires Santos
Horvath-Pereira, Bianca de Oliveira
da Silva-Júnior, Leandro Norberto
Tenório Fireman, João Víctor Barbosa
Mattar, Michel
Félix, Marcílio
Buchaim, Rogerio Leone
Carreira, Ana Claudia Oliveira
Miglino, Maria Angelica
Soares, Marcelo Melo
Three-Dimensional Printing of Graphene Oxide/Poly-L-Lactic Acid Scaffolds Using Fischer–Koch Modeling
title Three-Dimensional Printing of Graphene Oxide/Poly-L-Lactic Acid Scaffolds Using Fischer–Koch Modeling
title_full Three-Dimensional Printing of Graphene Oxide/Poly-L-Lactic Acid Scaffolds Using Fischer–Koch Modeling
title_fullStr Three-Dimensional Printing of Graphene Oxide/Poly-L-Lactic Acid Scaffolds Using Fischer–Koch Modeling
title_full_unstemmed Three-Dimensional Printing of Graphene Oxide/Poly-L-Lactic Acid Scaffolds Using Fischer–Koch Modeling
title_short Three-Dimensional Printing of Graphene Oxide/Poly-L-Lactic Acid Scaffolds Using Fischer–Koch Modeling
title_sort three-dimensional printing of graphene oxide/poly-l-lactic acid scaffolds using fischer–koch modeling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648465/
https://www.ncbi.nlm.nih.gov/pubmed/37959893
http://dx.doi.org/10.3390/polym15214213
work_keys_str_mv AT dasilvathamiressantos threedimensionalprintingofgrapheneoxidepolyllacticacidscaffoldsusingfischerkochmodeling
AT horvathpereirabiancadeoliveira threedimensionalprintingofgrapheneoxidepolyllacticacidscaffoldsusingfischerkochmodeling
AT dasilvajuniorleandronorberto threedimensionalprintingofgrapheneoxidepolyllacticacidscaffoldsusingfischerkochmodeling
AT tenoriofiremanjoaovictorbarbosa threedimensionalprintingofgrapheneoxidepolyllacticacidscaffoldsusingfischerkochmodeling
AT mattarmichel threedimensionalprintingofgrapheneoxidepolyllacticacidscaffoldsusingfischerkochmodeling
AT felixmarcilio threedimensionalprintingofgrapheneoxidepolyllacticacidscaffoldsusingfischerkochmodeling
AT buchaimrogerioleone threedimensionalprintingofgrapheneoxidepolyllacticacidscaffoldsusingfischerkochmodeling
AT carreiraanaclaudiaoliveira threedimensionalprintingofgrapheneoxidepolyllacticacidscaffoldsusingfischerkochmodeling
AT miglinomariaangelica threedimensionalprintingofgrapheneoxidepolyllacticacidscaffoldsusingfischerkochmodeling
AT soaresmarcelomelo threedimensionalprintingofgrapheneoxidepolyllacticacidscaffoldsusingfischerkochmodeling