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Vaginal Microbiota Molecular Profiling in Women with Bacterial Vaginosis: A Novel Diagnostic Tool
Bacterial vaginosis (BV) is a most common microbiological syndrome. Multiplex next-generation sequencing (NGS) or molecular tests allow a complete and accurate vaginal microbiota profiling in order to determine the primary causative agent. Due to the high costs and limited availability of NGS, the m...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10649576/ https://www.ncbi.nlm.nih.gov/pubmed/37958862 http://dx.doi.org/10.3390/ijms242115880 |
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author | Savicheva, Alevtina M. Krysanova, Anna A. Budilovskaya, Olga V. Spasibova, Elena V. Khusnutdinova, Tatiana A. Shalepo, Kira V. Beliaeva, Natalia R. Safarian, Galina Kh. Sapozhnikov, Kirill V. Tapilskaya, Natalya I. Kogan, Igor Yu. |
author_facet | Savicheva, Alevtina M. Krysanova, Anna A. Budilovskaya, Olga V. Spasibova, Elena V. Khusnutdinova, Tatiana A. Shalepo, Kira V. Beliaeva, Natalia R. Safarian, Galina Kh. Sapozhnikov, Kirill V. Tapilskaya, Natalya I. Kogan, Igor Yu. |
author_sort | Savicheva, Alevtina M. |
collection | PubMed |
description | Bacterial vaginosis (BV) is a most common microbiological syndrome. Multiplex next-generation sequencing (NGS) or molecular tests allow a complete and accurate vaginal microbiota profiling in order to determine the primary causative agent. Due to the high costs and limited availability of NGS, the multiplex real-time PCR draws more attention. The present study aimed to evaluate the microbial composition and dominant lactobacilli species in non-pregnant women with bacterial vaginosis using a multiplex RT-PCR test and determine its diagnostic significance. In total, 331 women complaining of vaginal discharge were included. BV was confirmed upon clinical examination and Nugent criteria. A real-time PCR test was carried out with a new Femoflor test, which identifies opportunistic bacteria, STD pathogens, and some viruses. According to the results, the rate of lactobacilli is significantly reduced in BV-affected patients when compared to healthy women. Moreover, the rate of L. crispatus significantly decreases, while the rate of L. iners remains high. Among obligate anaerobic bacteria, Gardnerella vaginalis was the most prevalent in women with BV. The Femoflor test demonstrated high sensitivity and specificity for diagnosing BV. Moreover, the test allows the identification of infection in women with intermediate vaginal microbiota, as well as STD pathogens, and viruses. Thus, the application of real-time PCR tests can be effectively used in vaginal microbiota evaluation in women with BV, intermediate vaginal microbiota, and healthy women. In addition, this test may be used as an alternative to the Amsel criteria and Nugent scoring method in diagnosing BV. |
format | Online Article Text |
id | pubmed-10649576 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106495762023-11-01 Vaginal Microbiota Molecular Profiling in Women with Bacterial Vaginosis: A Novel Diagnostic Tool Savicheva, Alevtina M. Krysanova, Anna A. Budilovskaya, Olga V. Spasibova, Elena V. Khusnutdinova, Tatiana A. Shalepo, Kira V. Beliaeva, Natalia R. Safarian, Galina Kh. Sapozhnikov, Kirill V. Tapilskaya, Natalya I. Kogan, Igor Yu. Int J Mol Sci Article Bacterial vaginosis (BV) is a most common microbiological syndrome. Multiplex next-generation sequencing (NGS) or molecular tests allow a complete and accurate vaginal microbiota profiling in order to determine the primary causative agent. Due to the high costs and limited availability of NGS, the multiplex real-time PCR draws more attention. The present study aimed to evaluate the microbial composition and dominant lactobacilli species in non-pregnant women with bacterial vaginosis using a multiplex RT-PCR test and determine its diagnostic significance. In total, 331 women complaining of vaginal discharge were included. BV was confirmed upon clinical examination and Nugent criteria. A real-time PCR test was carried out with a new Femoflor test, which identifies opportunistic bacteria, STD pathogens, and some viruses. According to the results, the rate of lactobacilli is significantly reduced in BV-affected patients when compared to healthy women. Moreover, the rate of L. crispatus significantly decreases, while the rate of L. iners remains high. Among obligate anaerobic bacteria, Gardnerella vaginalis was the most prevalent in women with BV. The Femoflor test demonstrated high sensitivity and specificity for diagnosing BV. Moreover, the test allows the identification of infection in women with intermediate vaginal microbiota, as well as STD pathogens, and viruses. Thus, the application of real-time PCR tests can be effectively used in vaginal microbiota evaluation in women with BV, intermediate vaginal microbiota, and healthy women. In addition, this test may be used as an alternative to the Amsel criteria and Nugent scoring method in diagnosing BV. MDPI 2023-11-01 /pmc/articles/PMC10649576/ /pubmed/37958862 http://dx.doi.org/10.3390/ijms242115880 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Savicheva, Alevtina M. Krysanova, Anna A. Budilovskaya, Olga V. Spasibova, Elena V. Khusnutdinova, Tatiana A. Shalepo, Kira V. Beliaeva, Natalia R. Safarian, Galina Kh. Sapozhnikov, Kirill V. Tapilskaya, Natalya I. Kogan, Igor Yu. Vaginal Microbiota Molecular Profiling in Women with Bacterial Vaginosis: A Novel Diagnostic Tool |
title | Vaginal Microbiota Molecular Profiling in Women with Bacterial Vaginosis: A Novel Diagnostic Tool |
title_full | Vaginal Microbiota Molecular Profiling in Women with Bacterial Vaginosis: A Novel Diagnostic Tool |
title_fullStr | Vaginal Microbiota Molecular Profiling in Women with Bacterial Vaginosis: A Novel Diagnostic Tool |
title_full_unstemmed | Vaginal Microbiota Molecular Profiling in Women with Bacterial Vaginosis: A Novel Diagnostic Tool |
title_short | Vaginal Microbiota Molecular Profiling in Women with Bacterial Vaginosis: A Novel Diagnostic Tool |
title_sort | vaginal microbiota molecular profiling in women with bacterial vaginosis: a novel diagnostic tool |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10649576/ https://www.ncbi.nlm.nih.gov/pubmed/37958862 http://dx.doi.org/10.3390/ijms242115880 |
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