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Genetic Mapping for QTL Associated with Seed Nickel and Molybdenum Accumulation in the Soybean ‘Forrest’ by ‘Williams 82’ RIL Population

Understanding the genetic basis of seed Ni and Mo is essential. Since soybean is a major crop in the world and a major source for nutrients, including Ni and Mo, the objective of the current research was to map genetic regions (quantitative trait loci, QTL) linked to Ni and Mo concentrations in soyb...

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Autores principales: Bellaloui, Nacer, Knizia, Dounya, Yuan, Jiazheng, Song, Qijian, Betts, Frances, Register, Teresa, Williams, Earl, Lakhssassi, Naoufal, Mazouz, Hamid, Nguyen, Henry T., Meksem, Khalid, Mengistu, Alemu, Kassem, My Abdelmajid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10649706/
https://www.ncbi.nlm.nih.gov/pubmed/37960065
http://dx.doi.org/10.3390/plants12213709
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author Bellaloui, Nacer
Knizia, Dounya
Yuan, Jiazheng
Song, Qijian
Betts, Frances
Register, Teresa
Williams, Earl
Lakhssassi, Naoufal
Mazouz, Hamid
Nguyen, Henry T.
Meksem, Khalid
Mengistu, Alemu
Kassem, My Abdelmajid
author_facet Bellaloui, Nacer
Knizia, Dounya
Yuan, Jiazheng
Song, Qijian
Betts, Frances
Register, Teresa
Williams, Earl
Lakhssassi, Naoufal
Mazouz, Hamid
Nguyen, Henry T.
Meksem, Khalid
Mengistu, Alemu
Kassem, My Abdelmajid
author_sort Bellaloui, Nacer
collection PubMed
description Understanding the genetic basis of seed Ni and Mo is essential. Since soybean is a major crop in the world and a major source for nutrients, including Ni and Mo, the objective of the current research was to map genetic regions (quantitative trait loci, QTL) linked to Ni and Mo concentrations in soybean seed. A recombinant inbred line (RIL) population was derived from a cross between ‘Forrest’ and ‘Williams 82’ (F × W82). A total of 306 lines was used for genotyping using 5405 single nucleotides polymorphism (SNP) markers using Infinium SNP6K BeadChips. A two-year experiment was conducted and included the parents and the RIL population. One experiment was conducted in 2018 in North Carolina (NC), and the second experiment was conducted in Illinois in 2020 (IL). Logarithm of the odds (LOD) of ≥2.5 was set as a threshold to report identified QTL using the composite interval mapping (CIM) method. A wide range of Ni and Mo concentrations among RILs was observed. A total of four QTL (qNi-01, qNi-02, and qNi-03 on Chr 2, 8, and 9, respectively, in 2018, and qNi-01 on Chr 20 in 2020) was identified for seed Ni. All these QTL were significantly (LOD threshold > 2.5) associated with seed Ni, with LOD scores ranging between 2.71–3.44, and with phenotypic variance ranging from 4.48–6.97%. A total of three QTL for Mo (qMo-01, qMo-02, and qMo-03 on Chr 1, 3, 17, respectively) was identified in 2018, and four QTL (qMo-01, qMo-02, qMo-03, and qMo-04, on Chr 5, 11, 14, and 16, respectively) were identified in 2020. Some of the current QTL had high LOD and significantly contributed to the phenotypic variance for the trait. For example, in 2018, Mo QTL qMo-01 on Chr 1 had LOD of 7.8, explaining a phenotypic variance of 41.17%, and qMo-03 on Chr 17 had LOD of 5.33, with phenotypic variance explained of 41.49%. In addition, one Mo QTL (qMo-03 on Chr 14) had LOD of 9.77, explaining 51.57% of phenotypic variance related to the trait, and another Mo QTL (qMo-04 on Chr 16) had LOD of 7.62 and explained 49.95% of phenotypic variance. None of the QTL identified here were identified twice across locations/years. Based on a search of the available literature and of SoyBase, the four QTL for Ni, identified on Chr 2, 8, 9, and 20, and the five QTL associated with Mo, identified on Chr 1, 17, 11, 14, and 16, are novel and not previously reported. This research contributes new insights into the genetic mapping of Ni and Mo, and provides valuable QTL and molecular markers that can potentially assist in selecting Ni and Mo levels in soybean seeds.
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spelling pubmed-106497062023-10-28 Genetic Mapping for QTL Associated with Seed Nickel and Molybdenum Accumulation in the Soybean ‘Forrest’ by ‘Williams 82’ RIL Population Bellaloui, Nacer Knizia, Dounya Yuan, Jiazheng Song, Qijian Betts, Frances Register, Teresa Williams, Earl Lakhssassi, Naoufal Mazouz, Hamid Nguyen, Henry T. Meksem, Khalid Mengistu, Alemu Kassem, My Abdelmajid Plants (Basel) Article Understanding the genetic basis of seed Ni and Mo is essential. Since soybean is a major crop in the world and a major source for nutrients, including Ni and Mo, the objective of the current research was to map genetic regions (quantitative trait loci, QTL) linked to Ni and Mo concentrations in soybean seed. A recombinant inbred line (RIL) population was derived from a cross between ‘Forrest’ and ‘Williams 82’ (F × W82). A total of 306 lines was used for genotyping using 5405 single nucleotides polymorphism (SNP) markers using Infinium SNP6K BeadChips. A two-year experiment was conducted and included the parents and the RIL population. One experiment was conducted in 2018 in North Carolina (NC), and the second experiment was conducted in Illinois in 2020 (IL). Logarithm of the odds (LOD) of ≥2.5 was set as a threshold to report identified QTL using the composite interval mapping (CIM) method. A wide range of Ni and Mo concentrations among RILs was observed. A total of four QTL (qNi-01, qNi-02, and qNi-03 on Chr 2, 8, and 9, respectively, in 2018, and qNi-01 on Chr 20 in 2020) was identified for seed Ni. All these QTL were significantly (LOD threshold > 2.5) associated with seed Ni, with LOD scores ranging between 2.71–3.44, and with phenotypic variance ranging from 4.48–6.97%. A total of three QTL for Mo (qMo-01, qMo-02, and qMo-03 on Chr 1, 3, 17, respectively) was identified in 2018, and four QTL (qMo-01, qMo-02, qMo-03, and qMo-04, on Chr 5, 11, 14, and 16, respectively) were identified in 2020. Some of the current QTL had high LOD and significantly contributed to the phenotypic variance for the trait. For example, in 2018, Mo QTL qMo-01 on Chr 1 had LOD of 7.8, explaining a phenotypic variance of 41.17%, and qMo-03 on Chr 17 had LOD of 5.33, with phenotypic variance explained of 41.49%. In addition, one Mo QTL (qMo-03 on Chr 14) had LOD of 9.77, explaining 51.57% of phenotypic variance related to the trait, and another Mo QTL (qMo-04 on Chr 16) had LOD of 7.62 and explained 49.95% of phenotypic variance. None of the QTL identified here were identified twice across locations/years. Based on a search of the available literature and of SoyBase, the four QTL for Ni, identified on Chr 2, 8, 9, and 20, and the five QTL associated with Mo, identified on Chr 1, 17, 11, 14, and 16, are novel and not previously reported. This research contributes new insights into the genetic mapping of Ni and Mo, and provides valuable QTL and molecular markers that can potentially assist in selecting Ni and Mo levels in soybean seeds. MDPI 2023-10-28 /pmc/articles/PMC10649706/ /pubmed/37960065 http://dx.doi.org/10.3390/plants12213709 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bellaloui, Nacer
Knizia, Dounya
Yuan, Jiazheng
Song, Qijian
Betts, Frances
Register, Teresa
Williams, Earl
Lakhssassi, Naoufal
Mazouz, Hamid
Nguyen, Henry T.
Meksem, Khalid
Mengistu, Alemu
Kassem, My Abdelmajid
Genetic Mapping for QTL Associated with Seed Nickel and Molybdenum Accumulation in the Soybean ‘Forrest’ by ‘Williams 82’ RIL Population
title Genetic Mapping for QTL Associated with Seed Nickel and Molybdenum Accumulation in the Soybean ‘Forrest’ by ‘Williams 82’ RIL Population
title_full Genetic Mapping for QTL Associated with Seed Nickel and Molybdenum Accumulation in the Soybean ‘Forrest’ by ‘Williams 82’ RIL Population
title_fullStr Genetic Mapping for QTL Associated with Seed Nickel and Molybdenum Accumulation in the Soybean ‘Forrest’ by ‘Williams 82’ RIL Population
title_full_unstemmed Genetic Mapping for QTL Associated with Seed Nickel and Molybdenum Accumulation in the Soybean ‘Forrest’ by ‘Williams 82’ RIL Population
title_short Genetic Mapping for QTL Associated with Seed Nickel and Molybdenum Accumulation in the Soybean ‘Forrest’ by ‘Williams 82’ RIL Population
title_sort genetic mapping for qtl associated with seed nickel and molybdenum accumulation in the soybean ‘forrest’ by ‘williams 82’ ril population
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10649706/
https://www.ncbi.nlm.nih.gov/pubmed/37960065
http://dx.doi.org/10.3390/plants12213709
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