Cargando…
Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India
The consumption of milk contaminated with antibiotic-resistant bacteria poses a significant health threat to humans. This study aimed to investigate the prevalence of Enterobacteriaceae producing β-lactamases (ESBL, MBL, and AmpC) in cow and buffalo milk samples from two Indian states, Haryana and A...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650101/ https://www.ncbi.nlm.nih.gov/pubmed/37760745 http://dx.doi.org/10.3390/antibiotics12091449 |
_version_ | 1785135702681321472 |
---|---|
author | Dey, Tushar Kumar Lindahl, Johanna Frida Lundkvist, Åke Grace, Delia Deka, Ram Pratim Shome, Rajeswari Bandyopadhyay, Samiran Goyal, Naresh Kumar Sharma, Garima Shome, Bibek Ranjan |
author_facet | Dey, Tushar Kumar Lindahl, Johanna Frida Lundkvist, Åke Grace, Delia Deka, Ram Pratim Shome, Rajeswari Bandyopadhyay, Samiran Goyal, Naresh Kumar Sharma, Garima Shome, Bibek Ranjan |
author_sort | Dey, Tushar Kumar |
collection | PubMed |
description | The consumption of milk contaminated with antibiotic-resistant bacteria poses a significant health threat to humans. This study aimed to investigate the prevalence of Enterobacteriaceae producing β-lactamases (ESBL, MBL, and AmpC) in cow and buffalo milk samples from two Indian states, Haryana and Assam. A total of 401 milk samples were collected from dairy farmers and vendors in the specified districts. Microbiological assays, antibiotic susceptibility testing, and PCR-based genotyping were employed to analyze 421 Gram-negative bacterial isolates. The overall prevalence of β-lactamase genes was 10% (confidence interval (CI) (7–13)), with higher rates in Haryana (13%, CI (9–19)) compared to Assam (7%, CI (4–11)). The identified β-lactamase genes in isolates were bla(CMY), bla(MOX), bla(FOX), bla(EBC), and bla(DHA), associated with AmpC production. Additionally, bla(CTX-M1), bla(SHV), and bla(TEM) were detected as ESBL producers, while bla(VIM), bla(IMP), bla(SPM), bla(SIM), and bla(GIM) were identified as MBL producers. Notably, Shigella spp. were the dominant β-lactamase producers among identified Enterobacteriaceae. This study highlights the presence of various prevalent β-lactamase genes in milk isolates, indicating the potential risk of antimicrobial-resistant bacteria in dairy products. The presence of β-lactam resistance raises concern as this could restrict antibiotic options for treatment. The discordance between genotypic and phenotypic methods emphasizes the necessity for comprehensive approaches that integrate both techniques to accurately assess antibiotic resistance. Urgent collaborative action incorporating rational and regulated use of antibiotics across the dairy value chain is required to address the global challenge of β-lactam resistance. |
format | Online Article Text |
id | pubmed-10650101 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106501012023-09-14 Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India Dey, Tushar Kumar Lindahl, Johanna Frida Lundkvist, Åke Grace, Delia Deka, Ram Pratim Shome, Rajeswari Bandyopadhyay, Samiran Goyal, Naresh Kumar Sharma, Garima Shome, Bibek Ranjan Antibiotics (Basel) Article The consumption of milk contaminated with antibiotic-resistant bacteria poses a significant health threat to humans. This study aimed to investigate the prevalence of Enterobacteriaceae producing β-lactamases (ESBL, MBL, and AmpC) in cow and buffalo milk samples from two Indian states, Haryana and Assam. A total of 401 milk samples were collected from dairy farmers and vendors in the specified districts. Microbiological assays, antibiotic susceptibility testing, and PCR-based genotyping were employed to analyze 421 Gram-negative bacterial isolates. The overall prevalence of β-lactamase genes was 10% (confidence interval (CI) (7–13)), with higher rates in Haryana (13%, CI (9–19)) compared to Assam (7%, CI (4–11)). The identified β-lactamase genes in isolates were bla(CMY), bla(MOX), bla(FOX), bla(EBC), and bla(DHA), associated with AmpC production. Additionally, bla(CTX-M1), bla(SHV), and bla(TEM) were detected as ESBL producers, while bla(VIM), bla(IMP), bla(SPM), bla(SIM), and bla(GIM) were identified as MBL producers. Notably, Shigella spp. were the dominant β-lactamase producers among identified Enterobacteriaceae. This study highlights the presence of various prevalent β-lactamase genes in milk isolates, indicating the potential risk of antimicrobial-resistant bacteria in dairy products. The presence of β-lactam resistance raises concern as this could restrict antibiotic options for treatment. The discordance between genotypic and phenotypic methods emphasizes the necessity for comprehensive approaches that integrate both techniques to accurately assess antibiotic resistance. Urgent collaborative action incorporating rational and regulated use of antibiotics across the dairy value chain is required to address the global challenge of β-lactam resistance. MDPI 2023-09-14 /pmc/articles/PMC10650101/ /pubmed/37760745 http://dx.doi.org/10.3390/antibiotics12091449 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dey, Tushar Kumar Lindahl, Johanna Frida Lundkvist, Åke Grace, Delia Deka, Ram Pratim Shome, Rajeswari Bandyopadhyay, Samiran Goyal, Naresh Kumar Sharma, Garima Shome, Bibek Ranjan Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India |
title | Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India |
title_full | Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India |
title_fullStr | Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India |
title_full_unstemmed | Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India |
title_short | Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India |
title_sort | analyses of extended-spectrum-β-lactamase, metallo-β-lactamase, and ampc-β-lactamase producing enterobacteriaceae from the dairy value chain in india |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650101/ https://www.ncbi.nlm.nih.gov/pubmed/37760745 http://dx.doi.org/10.3390/antibiotics12091449 |
work_keys_str_mv | AT deytusharkumar analysesofextendedspectrumblactamasemetalloblactamaseandampcblactamaseproducingenterobacteriaceaefromthedairyvaluechaininindia AT lindahljohannafrida analysesofextendedspectrumblactamasemetalloblactamaseandampcblactamaseproducingenterobacteriaceaefromthedairyvaluechaininindia AT lundkvistake analysesofextendedspectrumblactamasemetalloblactamaseandampcblactamaseproducingenterobacteriaceaefromthedairyvaluechaininindia AT gracedelia analysesofextendedspectrumblactamasemetalloblactamaseandampcblactamaseproducingenterobacteriaceaefromthedairyvaluechaininindia AT dekarampratim analysesofextendedspectrumblactamasemetalloblactamaseandampcblactamaseproducingenterobacteriaceaefromthedairyvaluechaininindia AT shomerajeswari analysesofextendedspectrumblactamasemetalloblactamaseandampcblactamaseproducingenterobacteriaceaefromthedairyvaluechaininindia AT bandyopadhyaysamiran analysesofextendedspectrumblactamasemetalloblactamaseandampcblactamaseproducingenterobacteriaceaefromthedairyvaluechaininindia AT goyalnareshkumar analysesofextendedspectrumblactamasemetalloblactamaseandampcblactamaseproducingenterobacteriaceaefromthedairyvaluechaininindia AT sharmagarima analysesofextendedspectrumblactamasemetalloblactamaseandampcblactamaseproducingenterobacteriaceaefromthedairyvaluechaininindia AT shomebibekranjan analysesofextendedspectrumblactamasemetalloblactamaseandampcblactamaseproducingenterobacteriaceaefromthedairyvaluechaininindia |