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Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India

The consumption of milk contaminated with antibiotic-resistant bacteria poses a significant health threat to humans. This study aimed to investigate the prevalence of Enterobacteriaceae producing β-lactamases (ESBL, MBL, and AmpC) in cow and buffalo milk samples from two Indian states, Haryana and A...

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Autores principales: Dey, Tushar Kumar, Lindahl, Johanna Frida, Lundkvist, Åke, Grace, Delia, Deka, Ram Pratim, Shome, Rajeswari, Bandyopadhyay, Samiran, Goyal, Naresh Kumar, Sharma, Garima, Shome, Bibek Ranjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650101/
https://www.ncbi.nlm.nih.gov/pubmed/37760745
http://dx.doi.org/10.3390/antibiotics12091449
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author Dey, Tushar Kumar
Lindahl, Johanna Frida
Lundkvist, Åke
Grace, Delia
Deka, Ram Pratim
Shome, Rajeswari
Bandyopadhyay, Samiran
Goyal, Naresh Kumar
Sharma, Garima
Shome, Bibek Ranjan
author_facet Dey, Tushar Kumar
Lindahl, Johanna Frida
Lundkvist, Åke
Grace, Delia
Deka, Ram Pratim
Shome, Rajeswari
Bandyopadhyay, Samiran
Goyal, Naresh Kumar
Sharma, Garima
Shome, Bibek Ranjan
author_sort Dey, Tushar Kumar
collection PubMed
description The consumption of milk contaminated with antibiotic-resistant bacteria poses a significant health threat to humans. This study aimed to investigate the prevalence of Enterobacteriaceae producing β-lactamases (ESBL, MBL, and AmpC) in cow and buffalo milk samples from two Indian states, Haryana and Assam. A total of 401 milk samples were collected from dairy farmers and vendors in the specified districts. Microbiological assays, antibiotic susceptibility testing, and PCR-based genotyping were employed to analyze 421 Gram-negative bacterial isolates. The overall prevalence of β-lactamase genes was 10% (confidence interval (CI) (7–13)), with higher rates in Haryana (13%, CI (9–19)) compared to Assam (7%, CI (4–11)). The identified β-lactamase genes in isolates were bla(CMY), bla(MOX), bla(FOX), bla(EBC), and bla(DHA), associated with AmpC production. Additionally, bla(CTX-M1), bla(SHV), and bla(TEM) were detected as ESBL producers, while bla(VIM), bla(IMP), bla(SPM), bla(SIM), and bla(GIM) were identified as MBL producers. Notably, Shigella spp. were the dominant β-lactamase producers among identified Enterobacteriaceae. This study highlights the presence of various prevalent β-lactamase genes in milk isolates, indicating the potential risk of antimicrobial-resistant bacteria in dairy products. The presence of β-lactam resistance raises concern as this could restrict antibiotic options for treatment. The discordance between genotypic and phenotypic methods emphasizes the necessity for comprehensive approaches that integrate both techniques to accurately assess antibiotic resistance. Urgent collaborative action incorporating rational and regulated use of antibiotics across the dairy value chain is required to address the global challenge of β-lactam resistance.
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spelling pubmed-106501012023-09-14 Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India Dey, Tushar Kumar Lindahl, Johanna Frida Lundkvist, Åke Grace, Delia Deka, Ram Pratim Shome, Rajeswari Bandyopadhyay, Samiran Goyal, Naresh Kumar Sharma, Garima Shome, Bibek Ranjan Antibiotics (Basel) Article The consumption of milk contaminated with antibiotic-resistant bacteria poses a significant health threat to humans. This study aimed to investigate the prevalence of Enterobacteriaceae producing β-lactamases (ESBL, MBL, and AmpC) in cow and buffalo milk samples from two Indian states, Haryana and Assam. A total of 401 milk samples were collected from dairy farmers and vendors in the specified districts. Microbiological assays, antibiotic susceptibility testing, and PCR-based genotyping were employed to analyze 421 Gram-negative bacterial isolates. The overall prevalence of β-lactamase genes was 10% (confidence interval (CI) (7–13)), with higher rates in Haryana (13%, CI (9–19)) compared to Assam (7%, CI (4–11)). The identified β-lactamase genes in isolates were bla(CMY), bla(MOX), bla(FOX), bla(EBC), and bla(DHA), associated with AmpC production. Additionally, bla(CTX-M1), bla(SHV), and bla(TEM) were detected as ESBL producers, while bla(VIM), bla(IMP), bla(SPM), bla(SIM), and bla(GIM) were identified as MBL producers. Notably, Shigella spp. were the dominant β-lactamase producers among identified Enterobacteriaceae. This study highlights the presence of various prevalent β-lactamase genes in milk isolates, indicating the potential risk of antimicrobial-resistant bacteria in dairy products. The presence of β-lactam resistance raises concern as this could restrict antibiotic options for treatment. The discordance between genotypic and phenotypic methods emphasizes the necessity for comprehensive approaches that integrate both techniques to accurately assess antibiotic resistance. Urgent collaborative action incorporating rational and regulated use of antibiotics across the dairy value chain is required to address the global challenge of β-lactam resistance. MDPI 2023-09-14 /pmc/articles/PMC10650101/ /pubmed/37760745 http://dx.doi.org/10.3390/antibiotics12091449 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dey, Tushar Kumar
Lindahl, Johanna Frida
Lundkvist, Åke
Grace, Delia
Deka, Ram Pratim
Shome, Rajeswari
Bandyopadhyay, Samiran
Goyal, Naresh Kumar
Sharma, Garima
Shome, Bibek Ranjan
Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India
title Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India
title_full Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India
title_fullStr Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India
title_full_unstemmed Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India
title_short Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India
title_sort analyses of extended-spectrum-β-lactamase, metallo-β-lactamase, and ampc-β-lactamase producing enterobacteriaceae from the dairy value chain in india
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650101/
https://www.ncbi.nlm.nih.gov/pubmed/37760745
http://dx.doi.org/10.3390/antibiotics12091449
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