Cargando…

Searching for Virulence Factors among Staphylococcus lugdunensis Isolates from Orthopedic Infections: Correlation of β-hemolysin, hemolysin III, and slush Genes with Hemolytic Activity and Synergistic Hemolytic Activity

Staphylococcus lugdunensis is an emerging high-virulent pathogen. Here, the presence and expression of virulence genes (icaA, fbl, vwbl, fbpA, slush A, B and C, and genes of the putative β-hemolysin and hemolysin III) and the ability to induce synergistic hemolytic activity and hemolysis after 24, 4...

Descripción completa

Detalles Bibliográficos
Autores principales: Ravaioli, Stefano, Campoccia, Davide, Mirzaei, Rasoul, Mariani, Valentina, Bottau, Giulia, De Donno, Andrea, Montanaro, Lucio, Speziale, Pietro, Arciola, Carla Renata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650139/
https://www.ncbi.nlm.nih.gov/pubmed/37958706
http://dx.doi.org/10.3390/ijms242115724
_version_ 1785135711625674752
author Ravaioli, Stefano
Campoccia, Davide
Mirzaei, Rasoul
Mariani, Valentina
Bottau, Giulia
De Donno, Andrea
Montanaro, Lucio
Speziale, Pietro
Arciola, Carla Renata
author_facet Ravaioli, Stefano
Campoccia, Davide
Mirzaei, Rasoul
Mariani, Valentina
Bottau, Giulia
De Donno, Andrea
Montanaro, Lucio
Speziale, Pietro
Arciola, Carla Renata
author_sort Ravaioli, Stefano
collection PubMed
description Staphylococcus lugdunensis is an emerging high-virulent pathogen. Here, the presence and expression of virulence genes (icaA, fbl, vwbl, fbpA, slush A, B and C, and genes of the putative β-hemolysin and hemolysin III) and the ability to induce synergistic hemolytic activity and hemolysis after 24, 48 and 72 h were investigated in a collection of twenty-two S. lugdunensis clinical isolates. The collection of isolates, mainly from implant orthopedic infections, had previously been grouped by ribotyping/dendrogram analysis and studied for biofilm matrices, biomasses and antibiotic resistances. Two isolates, constituting a unique small ribogroup sharing the same cluster, exhibited an amplicon size of the slush operon (S. lugdunensis synergistic hemolysin) which was shorter than the expected 977 bp. This outcome can predict the genetic lineage of the S. lugdunensis strains. One isolate (cra1342) presented two deletions: one of 90 bp in slush A and the other of 91 bp in slush B. Another isolate (N860314) showed a single 193 bp deletion, which encompassed part of the slush B terminal sequence and most of slush C. The isolate N860314 was devoid of hemolytic activity after 24 h, and the first consideration was that the deleted region deals with the coding of the active enzymatic site of the slush hemolysin. On the other hand, cra1342 and N860314 isolates with different slush deletions and with hemolytic activity after 24 and 48 h, respectively, could have replaced the hemolytic phenotype through other processes.
format Online
Article
Text
id pubmed-10650139
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106501392023-10-29 Searching for Virulence Factors among Staphylococcus lugdunensis Isolates from Orthopedic Infections: Correlation of β-hemolysin, hemolysin III, and slush Genes with Hemolytic Activity and Synergistic Hemolytic Activity Ravaioli, Stefano Campoccia, Davide Mirzaei, Rasoul Mariani, Valentina Bottau, Giulia De Donno, Andrea Montanaro, Lucio Speziale, Pietro Arciola, Carla Renata Int J Mol Sci Article Staphylococcus lugdunensis is an emerging high-virulent pathogen. Here, the presence and expression of virulence genes (icaA, fbl, vwbl, fbpA, slush A, B and C, and genes of the putative β-hemolysin and hemolysin III) and the ability to induce synergistic hemolytic activity and hemolysis after 24, 48 and 72 h were investigated in a collection of twenty-two S. lugdunensis clinical isolates. The collection of isolates, mainly from implant orthopedic infections, had previously been grouped by ribotyping/dendrogram analysis and studied for biofilm matrices, biomasses and antibiotic resistances. Two isolates, constituting a unique small ribogroup sharing the same cluster, exhibited an amplicon size of the slush operon (S. lugdunensis synergistic hemolysin) which was shorter than the expected 977 bp. This outcome can predict the genetic lineage of the S. lugdunensis strains. One isolate (cra1342) presented two deletions: one of 90 bp in slush A and the other of 91 bp in slush B. Another isolate (N860314) showed a single 193 bp deletion, which encompassed part of the slush B terminal sequence and most of slush C. The isolate N860314 was devoid of hemolytic activity after 24 h, and the first consideration was that the deleted region deals with the coding of the active enzymatic site of the slush hemolysin. On the other hand, cra1342 and N860314 isolates with different slush deletions and with hemolytic activity after 24 and 48 h, respectively, could have replaced the hemolytic phenotype through other processes. MDPI 2023-10-29 /pmc/articles/PMC10650139/ /pubmed/37958706 http://dx.doi.org/10.3390/ijms242115724 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ravaioli, Stefano
Campoccia, Davide
Mirzaei, Rasoul
Mariani, Valentina
Bottau, Giulia
De Donno, Andrea
Montanaro, Lucio
Speziale, Pietro
Arciola, Carla Renata
Searching for Virulence Factors among Staphylococcus lugdunensis Isolates from Orthopedic Infections: Correlation of β-hemolysin, hemolysin III, and slush Genes with Hemolytic Activity and Synergistic Hemolytic Activity
title Searching for Virulence Factors among Staphylococcus lugdunensis Isolates from Orthopedic Infections: Correlation of β-hemolysin, hemolysin III, and slush Genes with Hemolytic Activity and Synergistic Hemolytic Activity
title_full Searching for Virulence Factors among Staphylococcus lugdunensis Isolates from Orthopedic Infections: Correlation of β-hemolysin, hemolysin III, and slush Genes with Hemolytic Activity and Synergistic Hemolytic Activity
title_fullStr Searching for Virulence Factors among Staphylococcus lugdunensis Isolates from Orthopedic Infections: Correlation of β-hemolysin, hemolysin III, and slush Genes with Hemolytic Activity and Synergistic Hemolytic Activity
title_full_unstemmed Searching for Virulence Factors among Staphylococcus lugdunensis Isolates from Orthopedic Infections: Correlation of β-hemolysin, hemolysin III, and slush Genes with Hemolytic Activity and Synergistic Hemolytic Activity
title_short Searching for Virulence Factors among Staphylococcus lugdunensis Isolates from Orthopedic Infections: Correlation of β-hemolysin, hemolysin III, and slush Genes with Hemolytic Activity and Synergistic Hemolytic Activity
title_sort searching for virulence factors among staphylococcus lugdunensis isolates from orthopedic infections: correlation of β-hemolysin, hemolysin iii, and slush genes with hemolytic activity and synergistic hemolytic activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650139/
https://www.ncbi.nlm.nih.gov/pubmed/37958706
http://dx.doi.org/10.3390/ijms242115724
work_keys_str_mv AT ravaiolistefano searchingforvirulencefactorsamongstaphylococcuslugdunensisisolatesfromorthopedicinfectionscorrelationofbhemolysinhemolysiniiiandslushgeneswithhemolyticactivityandsynergistichemolyticactivity
AT campocciadavide searchingforvirulencefactorsamongstaphylococcuslugdunensisisolatesfromorthopedicinfectionscorrelationofbhemolysinhemolysiniiiandslushgeneswithhemolyticactivityandsynergistichemolyticactivity
AT mirzaeirasoul searchingforvirulencefactorsamongstaphylococcuslugdunensisisolatesfromorthopedicinfectionscorrelationofbhemolysinhemolysiniiiandslushgeneswithhemolyticactivityandsynergistichemolyticactivity
AT marianivalentina searchingforvirulencefactorsamongstaphylococcuslugdunensisisolatesfromorthopedicinfectionscorrelationofbhemolysinhemolysiniiiandslushgeneswithhemolyticactivityandsynergistichemolyticactivity
AT bottaugiulia searchingforvirulencefactorsamongstaphylococcuslugdunensisisolatesfromorthopedicinfectionscorrelationofbhemolysinhemolysiniiiandslushgeneswithhemolyticactivityandsynergistichemolyticactivity
AT dedonnoandrea searchingforvirulencefactorsamongstaphylococcuslugdunensisisolatesfromorthopedicinfectionscorrelationofbhemolysinhemolysiniiiandslushgeneswithhemolyticactivityandsynergistichemolyticactivity
AT montanarolucio searchingforvirulencefactorsamongstaphylococcuslugdunensisisolatesfromorthopedicinfectionscorrelationofbhemolysinhemolysiniiiandslushgeneswithhemolyticactivityandsynergistichemolyticactivity
AT spezialepietro searchingforvirulencefactorsamongstaphylococcuslugdunensisisolatesfromorthopedicinfectionscorrelationofbhemolysinhemolysiniiiandslushgeneswithhemolyticactivityandsynergistichemolyticactivity
AT arciolacarlarenata searchingforvirulencefactorsamongstaphylococcuslugdunensisisolatesfromorthopedicinfectionscorrelationofbhemolysinhemolysiniiiandslushgeneswithhemolyticactivityandsynergistichemolyticactivity