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The Comparison of Serum Exosome Protein Profile in Diagnosis of NSCLC Patients

A thorough study of the exosomal proteomic cargo may enable the identification of proteins that play an important role in cancer development. The aim of this study was to compare the protein profiles of the serum exosomes derived from non-small lung cancer (NSCLC) patients and healthy volunteers (co...

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Autores principales: Baran, Kamila, Waśko, Joanna, Kryczka, Jakub, Boncela, Joanna, Jabłoński, Sławomir, Kolesińska, Beata, Brzeziańska-Lasota, Ewa, Kordiak, Jacek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650331/
https://www.ncbi.nlm.nih.gov/pubmed/37761972
http://dx.doi.org/10.3390/ijms241813669
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author Baran, Kamila
Waśko, Joanna
Kryczka, Jakub
Boncela, Joanna
Jabłoński, Sławomir
Kolesińska, Beata
Brzeziańska-Lasota, Ewa
Kordiak, Jacek
author_facet Baran, Kamila
Waśko, Joanna
Kryczka, Jakub
Boncela, Joanna
Jabłoński, Sławomir
Kolesińska, Beata
Brzeziańska-Lasota, Ewa
Kordiak, Jacek
author_sort Baran, Kamila
collection PubMed
description A thorough study of the exosomal proteomic cargo may enable the identification of proteins that play an important role in cancer development. The aim of this study was to compare the protein profiles of the serum exosomes derived from non-small lung cancer (NSCLC) patients and healthy volunteers (control) using the high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS) method to identify potentially new diagnostic and/or prognostic protein biomarkers. Proteins exclusively identified in NSCLC and control groups were analyzed using several bioinformatic tools and platforms (FunRich, Vesiclepedia, STRING, and TIMER2.0) to find key protein hubs involved in NSCLC progression and the acquisition of metastatic potential. This analysis revealed 150 NSCLC proteins, which are significantly involved in osmoregulation, cell–cell adhesion, cell motility, and differentiation. Among them, 3 proteins: Interleukin-34 (IL-34), HLA class II histocompatibility antigen, DM alpha chain (HLA-DMA), and HLA class II histocompatibility antigen, DO beta chain (HLA-DOB) were shown to be significantly involved in the cancer-associated fibroblasts (CAFs) and tumor-associated macrophages (TAMs) infiltration processes. Additionally, detected proteins were analyzed according to the presence of lymph node metastasis, showing that differences in frequency of detection of protein FAM166B, killer cell immunoglobulin-like receptor 2DL1, and olfactory receptor 52R1 correlate with the N feature according to the TNM Classification of Malignant Tumors. These results prove their involvement in NSCLC lymph node spread and metastasis. However, this study requires further investigation.
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spelling pubmed-106503312023-09-05 The Comparison of Serum Exosome Protein Profile in Diagnosis of NSCLC Patients Baran, Kamila Waśko, Joanna Kryczka, Jakub Boncela, Joanna Jabłoński, Sławomir Kolesińska, Beata Brzeziańska-Lasota, Ewa Kordiak, Jacek Int J Mol Sci Article A thorough study of the exosomal proteomic cargo may enable the identification of proteins that play an important role in cancer development. The aim of this study was to compare the protein profiles of the serum exosomes derived from non-small lung cancer (NSCLC) patients and healthy volunteers (control) using the high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS) method to identify potentially new diagnostic and/or prognostic protein biomarkers. Proteins exclusively identified in NSCLC and control groups were analyzed using several bioinformatic tools and platforms (FunRich, Vesiclepedia, STRING, and TIMER2.0) to find key protein hubs involved in NSCLC progression and the acquisition of metastatic potential. This analysis revealed 150 NSCLC proteins, which are significantly involved in osmoregulation, cell–cell adhesion, cell motility, and differentiation. Among them, 3 proteins: Interleukin-34 (IL-34), HLA class II histocompatibility antigen, DM alpha chain (HLA-DMA), and HLA class II histocompatibility antigen, DO beta chain (HLA-DOB) were shown to be significantly involved in the cancer-associated fibroblasts (CAFs) and tumor-associated macrophages (TAMs) infiltration processes. Additionally, detected proteins were analyzed according to the presence of lymph node metastasis, showing that differences in frequency of detection of protein FAM166B, killer cell immunoglobulin-like receptor 2DL1, and olfactory receptor 52R1 correlate with the N feature according to the TNM Classification of Malignant Tumors. These results prove their involvement in NSCLC lymph node spread and metastasis. However, this study requires further investigation. MDPI 2023-09-05 /pmc/articles/PMC10650331/ /pubmed/37761972 http://dx.doi.org/10.3390/ijms241813669 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baran, Kamila
Waśko, Joanna
Kryczka, Jakub
Boncela, Joanna
Jabłoński, Sławomir
Kolesińska, Beata
Brzeziańska-Lasota, Ewa
Kordiak, Jacek
The Comparison of Serum Exosome Protein Profile in Diagnosis of NSCLC Patients
title The Comparison of Serum Exosome Protein Profile in Diagnosis of NSCLC Patients
title_full The Comparison of Serum Exosome Protein Profile in Diagnosis of NSCLC Patients
title_fullStr The Comparison of Serum Exosome Protein Profile in Diagnosis of NSCLC Patients
title_full_unstemmed The Comparison of Serum Exosome Protein Profile in Diagnosis of NSCLC Patients
title_short The Comparison of Serum Exosome Protein Profile in Diagnosis of NSCLC Patients
title_sort comparison of serum exosome protein profile in diagnosis of nsclc patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650331/
https://www.ncbi.nlm.nih.gov/pubmed/37761972
http://dx.doi.org/10.3390/ijms241813669
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