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Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins
Dietary nucleic acids (dietNAs) are being increasingly recognized as important food components with nutritional value. However, the precise dietary recommendations for dietNAs are limited, because established methods for determining the quantity and nutritional role of dietNAs are still lacking. One...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650480/ https://www.ncbi.nlm.nih.gov/pubmed/37959061 http://dx.doi.org/10.3390/foods12213942 |
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author | Kościelak, Anna Koziara, Zuzanna Maria, Ana Pons Płatek, Rafał Bartoszek, Agnieszka |
author_facet | Kościelak, Anna Koziara, Zuzanna Maria, Ana Pons Płatek, Rafał Bartoszek, Agnieszka |
author_sort | Kościelak, Anna |
collection | PubMed |
description | Dietary nucleic acids (dietNAs) are being increasingly recognized as important food components with nutritional value. However, the precise dietary recommendations for dietNAs are limited, because established methods for determining the quantity and nutritional role of dietNAs are still lacking. One of the tools to narrow this gap could be microscopic imaging, as a convenient approach to visualize the abundance and distribution of dietNAs in food products. With the aid of appropriate bioinformatic elaboration, such images may in future enable the direct semiquantitative estimation of these macromolecules in food products. In the presented study, two methods of preparing microscopic sections and staining them with DNA-specific fluorochromes were used for microscopic imaging of dietNAs in food products of plant and animal origin. Procedures for preparing formalin-fixed paraffin-embedded sections and cryosections were compared in terms of their usefulness for routine food analysis. Both methods turned out equally suitable for visualizing dietNA distribution in animal and plant products. However, the use of cryosections allowed a significantly shorter analysis time and reduced the consumption of organic solvents. Both of these advantages make the cryosection method preferable while establishing a dedicated methodology for routine assessment of dietNAs in the food industry. |
format | Online Article Text |
id | pubmed-10650480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106504802023-10-28 Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins Kościelak, Anna Koziara, Zuzanna Maria, Ana Pons Płatek, Rafał Bartoszek, Agnieszka Foods Article Dietary nucleic acids (dietNAs) are being increasingly recognized as important food components with nutritional value. However, the precise dietary recommendations for dietNAs are limited, because established methods for determining the quantity and nutritional role of dietNAs are still lacking. One of the tools to narrow this gap could be microscopic imaging, as a convenient approach to visualize the abundance and distribution of dietNAs in food products. With the aid of appropriate bioinformatic elaboration, such images may in future enable the direct semiquantitative estimation of these macromolecules in food products. In the presented study, two methods of preparing microscopic sections and staining them with DNA-specific fluorochromes were used for microscopic imaging of dietNAs in food products of plant and animal origin. Procedures for preparing formalin-fixed paraffin-embedded sections and cryosections were compared in terms of their usefulness for routine food analysis. Both methods turned out equally suitable for visualizing dietNA distribution in animal and plant products. However, the use of cryosections allowed a significantly shorter analysis time and reduced the consumption of organic solvents. Both of these advantages make the cryosection method preferable while establishing a dedicated methodology for routine assessment of dietNAs in the food industry. MDPI 2023-10-28 /pmc/articles/PMC10650480/ /pubmed/37959061 http://dx.doi.org/10.3390/foods12213942 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kościelak, Anna Koziara, Zuzanna Maria, Ana Pons Płatek, Rafał Bartoszek, Agnieszka Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins |
title | Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins |
title_full | Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins |
title_fullStr | Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins |
title_full_unstemmed | Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins |
title_short | Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins |
title_sort | microscopic imaging to visualize the distribution of dietary nucleic acids in food products of various origins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650480/ https://www.ncbi.nlm.nih.gov/pubmed/37959061 http://dx.doi.org/10.3390/foods12213942 |
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