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Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins

Dietary nucleic acids (dietNAs) are being increasingly recognized as important food components with nutritional value. However, the precise dietary recommendations for dietNAs are limited, because established methods for determining the quantity and nutritional role of dietNAs are still lacking. One...

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Autores principales: Kościelak, Anna, Koziara, Zuzanna, Maria, Ana Pons, Płatek, Rafał, Bartoszek, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650480/
https://www.ncbi.nlm.nih.gov/pubmed/37959061
http://dx.doi.org/10.3390/foods12213942
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author Kościelak, Anna
Koziara, Zuzanna
Maria, Ana Pons
Płatek, Rafał
Bartoszek, Agnieszka
author_facet Kościelak, Anna
Koziara, Zuzanna
Maria, Ana Pons
Płatek, Rafał
Bartoszek, Agnieszka
author_sort Kościelak, Anna
collection PubMed
description Dietary nucleic acids (dietNAs) are being increasingly recognized as important food components with nutritional value. However, the precise dietary recommendations for dietNAs are limited, because established methods for determining the quantity and nutritional role of dietNAs are still lacking. One of the tools to narrow this gap could be microscopic imaging, as a convenient approach to visualize the abundance and distribution of dietNAs in food products. With the aid of appropriate bioinformatic elaboration, such images may in future enable the direct semiquantitative estimation of these macromolecules in food products. In the presented study, two methods of preparing microscopic sections and staining them with DNA-specific fluorochromes were used for microscopic imaging of dietNAs in food products of plant and animal origin. Procedures for preparing formalin-fixed paraffin-embedded sections and cryosections were compared in terms of their usefulness for routine food analysis. Both methods turned out equally suitable for visualizing dietNA distribution in animal and plant products. However, the use of cryosections allowed a significantly shorter analysis time and reduced the consumption of organic solvents. Both of these advantages make the cryosection method preferable while establishing a dedicated methodology for routine assessment of dietNAs in the food industry.
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spelling pubmed-106504802023-10-28 Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins Kościelak, Anna Koziara, Zuzanna Maria, Ana Pons Płatek, Rafał Bartoszek, Agnieszka Foods Article Dietary nucleic acids (dietNAs) are being increasingly recognized as important food components with nutritional value. However, the precise dietary recommendations for dietNAs are limited, because established methods for determining the quantity and nutritional role of dietNAs are still lacking. One of the tools to narrow this gap could be microscopic imaging, as a convenient approach to visualize the abundance and distribution of dietNAs in food products. With the aid of appropriate bioinformatic elaboration, such images may in future enable the direct semiquantitative estimation of these macromolecules in food products. In the presented study, two methods of preparing microscopic sections and staining them with DNA-specific fluorochromes were used for microscopic imaging of dietNAs in food products of plant and animal origin. Procedures for preparing formalin-fixed paraffin-embedded sections and cryosections were compared in terms of their usefulness for routine food analysis. Both methods turned out equally suitable for visualizing dietNA distribution in animal and plant products. However, the use of cryosections allowed a significantly shorter analysis time and reduced the consumption of organic solvents. Both of these advantages make the cryosection method preferable while establishing a dedicated methodology for routine assessment of dietNAs in the food industry. MDPI 2023-10-28 /pmc/articles/PMC10650480/ /pubmed/37959061 http://dx.doi.org/10.3390/foods12213942 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kościelak, Anna
Koziara, Zuzanna
Maria, Ana Pons
Płatek, Rafał
Bartoszek, Agnieszka
Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins
title Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins
title_full Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins
title_fullStr Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins
title_full_unstemmed Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins
title_short Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins
title_sort microscopic imaging to visualize the distribution of dietary nucleic acids in food products of various origins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650480/
https://www.ncbi.nlm.nih.gov/pubmed/37959061
http://dx.doi.org/10.3390/foods12213942
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