Cargando…

Albuminuria Is Affected by Urinary Tract Infection: A Comparison between Biochemical Quantitative Method and Automatic Urine Chemistry Analyzer UC-3500

The automated urine reagent strip test is a cost-effective tool for detecting albuminuria in patients. However, prior research has not investigated how urinary tract infections (UTIs) affect the test’s accuracy. Therefore, this study aims to assess the impact of UTIs on albuminuria diagnosis using b...

Descripción completa

Detalles Bibliográficos
Autores principales: Chou, Yi-Ju, Yang, Chun-Chun, Chang, Shang-Jen, Yang, Stephen Shei-Dei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650489/
https://www.ncbi.nlm.nih.gov/pubmed/37958262
http://dx.doi.org/10.3390/diagnostics13213366
_version_ 1785135791963373568
author Chou, Yi-Ju
Yang, Chun-Chun
Chang, Shang-Jen
Yang, Stephen Shei-Dei
author_facet Chou, Yi-Ju
Yang, Chun-Chun
Chang, Shang-Jen
Yang, Stephen Shei-Dei
author_sort Chou, Yi-Ju
collection PubMed
description The automated urine reagent strip test is a cost-effective tool for detecting albuminuria in patients. However, prior research has not investigated how urinary tract infections (UTIs) affect the test’s accuracy. Therefore, this study aims to assess the impact of UTIs on albuminuria diagnosis using both the biochemical quantitative method and the test strip method of the Fully Automatic Urine Chemistry Analyzer, UC-3500 (Sysmex, Kobe, Japan). From March to December 2019, we prospectively collected midstream urine from adult female UTI patients before and after one week of cephalexin treatment. The urine samples were subjected to culture, routine urinalysis, and albuminuria diagnosis using the biochemical quantitative method and UC-3500. Albuminuria was defined as a urine albumin to creatinine ratio (UACR) ≥ 30 mg/g in the biochemical quantitative method. The results were compared between the two methods. Among fifty-four female patients (average age: 50.5 ± 4.4 years) with UTIs, 24 (44.44%) had transient albuminuria. The quantitative UACR significantly decreased after one week of antibiotic treatment (median: 53 mg/g to 9 mg/g; median difference: −0.54, p < 0.0001). UC-3500 exhibited a higher false positive rate for diagnosing albuminuria during UTIs (42%) compared to after treatment (19%). Its agreement with the biochemical quantitative method was moderate during UTI (κ = 0.49, 95% confidence interval [CI]: 0.24–0.73) and good after treatment (κ = 0.65, 95% CI: 0.45–0.86). UC-3500’s accuracy in diagnosing albuminuria is influenced by UTIs, leading to either transient albuminuria or a false positive reaction of the test strip. UTI should be excluded or treated before its application in albuminuria screening.
format Online
Article
Text
id pubmed-10650489
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106504892023-11-02 Albuminuria Is Affected by Urinary Tract Infection: A Comparison between Biochemical Quantitative Method and Automatic Urine Chemistry Analyzer UC-3500 Chou, Yi-Ju Yang, Chun-Chun Chang, Shang-Jen Yang, Stephen Shei-Dei Diagnostics (Basel) Article The automated urine reagent strip test is a cost-effective tool for detecting albuminuria in patients. However, prior research has not investigated how urinary tract infections (UTIs) affect the test’s accuracy. Therefore, this study aims to assess the impact of UTIs on albuminuria diagnosis using both the biochemical quantitative method and the test strip method of the Fully Automatic Urine Chemistry Analyzer, UC-3500 (Sysmex, Kobe, Japan). From March to December 2019, we prospectively collected midstream urine from adult female UTI patients before and after one week of cephalexin treatment. The urine samples were subjected to culture, routine urinalysis, and albuminuria diagnosis using the biochemical quantitative method and UC-3500. Albuminuria was defined as a urine albumin to creatinine ratio (UACR) ≥ 30 mg/g in the biochemical quantitative method. The results were compared between the two methods. Among fifty-four female patients (average age: 50.5 ± 4.4 years) with UTIs, 24 (44.44%) had transient albuminuria. The quantitative UACR significantly decreased after one week of antibiotic treatment (median: 53 mg/g to 9 mg/g; median difference: −0.54, p < 0.0001). UC-3500 exhibited a higher false positive rate for diagnosing albuminuria during UTIs (42%) compared to after treatment (19%). Its agreement with the biochemical quantitative method was moderate during UTI (κ = 0.49, 95% confidence interval [CI]: 0.24–0.73) and good after treatment (κ = 0.65, 95% CI: 0.45–0.86). UC-3500’s accuracy in diagnosing albuminuria is influenced by UTIs, leading to either transient albuminuria or a false positive reaction of the test strip. UTI should be excluded or treated before its application in albuminuria screening. MDPI 2023-11-02 /pmc/articles/PMC10650489/ /pubmed/37958262 http://dx.doi.org/10.3390/diagnostics13213366 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chou, Yi-Ju
Yang, Chun-Chun
Chang, Shang-Jen
Yang, Stephen Shei-Dei
Albuminuria Is Affected by Urinary Tract Infection: A Comparison between Biochemical Quantitative Method and Automatic Urine Chemistry Analyzer UC-3500
title Albuminuria Is Affected by Urinary Tract Infection: A Comparison between Biochemical Quantitative Method and Automatic Urine Chemistry Analyzer UC-3500
title_full Albuminuria Is Affected by Urinary Tract Infection: A Comparison between Biochemical Quantitative Method and Automatic Urine Chemistry Analyzer UC-3500
title_fullStr Albuminuria Is Affected by Urinary Tract Infection: A Comparison between Biochemical Quantitative Method and Automatic Urine Chemistry Analyzer UC-3500
title_full_unstemmed Albuminuria Is Affected by Urinary Tract Infection: A Comparison between Biochemical Quantitative Method and Automatic Urine Chemistry Analyzer UC-3500
title_short Albuminuria Is Affected by Urinary Tract Infection: A Comparison between Biochemical Quantitative Method and Automatic Urine Chemistry Analyzer UC-3500
title_sort albuminuria is affected by urinary tract infection: a comparison between biochemical quantitative method and automatic urine chemistry analyzer uc-3500
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650489/
https://www.ncbi.nlm.nih.gov/pubmed/37958262
http://dx.doi.org/10.3390/diagnostics13213366
work_keys_str_mv AT chouyiju albuminuriaisaffectedbyurinarytractinfectionacomparisonbetweenbiochemicalquantitativemethodandautomaticurinechemistryanalyzeruc3500
AT yangchunchun albuminuriaisaffectedbyurinarytractinfectionacomparisonbetweenbiochemicalquantitativemethodandautomaticurinechemistryanalyzeruc3500
AT changshangjen albuminuriaisaffectedbyurinarytractinfectionacomparisonbetweenbiochemicalquantitativemethodandautomaticurinechemistryanalyzeruc3500
AT yangstephensheidei albuminuriaisaffectedbyurinarytractinfectionacomparisonbetweenbiochemicalquantitativemethodandautomaticurinechemistryanalyzeruc3500